General Information

Product

Smad1 Antibody Affinity Purified

Description

Affinity Purified Mothers against dpp Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 405-455 on D. melanogaster Mothers against dpp protein.

Accession #

NCBI:  NP_477017.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P42003

Overview

Required for the function of decapentaplegic. May play an important role in mediating Dpp signaling. Involved in the BMP signaling pathway.

Molecular Function

DNA binding; RNA polymerase II activating transcription factor; Transcription regulatory region sequence-specific activity; Transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Subcellular Location

Cytoplasm; Nucleus

Expression

Is detected in all developmental stages, though it appears most abundant in pupae, adult stages and early embryos. Its abundance decreases throughout embryonic and larval development and then returns to high levels in pupae and adult females.

Structure

Homotrimer

Alternative Nomenclature

BSP 1 antibody

Dwarfin A antibody

DwfA antibody

JV 41 antibody

MAD homolog 1 antibody

MAD mothers against decapentaplegic homolog 1 antibody

Mad related protein 1 antibody

MADR1 antibody

Mothers against DPP homolog 1 antibody

Sma and Mad related protein 1 antibody

SMAD 1 antibody

Spinal muscular atrophy distal with upper limb predominance antibody

TGF beta signaling protein 1 antibody

Transforming growth factor beta signaling protein 1 antibody

General Information

Product

alpha Synuclein Antibody

Description

Affinity Purified alpha-synuclein isoform NACP112 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 62-112 unique sequence on alpha-synuclein isoform NACP 112 protein.

Accession #

NCBI: NP_009292

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

P37840

Overview

May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

Molecular Function

Alpha-tubulin binding, Calcium-ion binding, copper ion binding

Subcellular Location

cytoplasm, Membrane, Nucleus, Cell junction > synapse

Expression

Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.

Structure

Soluble monomer which can form filamentous aggregates. Interacts with UCHL1. Interacts with phospholipase D and histones

Alternative Nomenclature

Alpha synuclein 

MGC105443 

MGC110988 

MGC127560 

NACP 

PARK 1 

PARK 4 

PD 1 

SNCA 

SYN 

General Information

Product

Nogo Antibody

Description

Affinity Purified Reticulon-4 isoform A Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1100-1200 on Nogo AS protein.

Accession #

NCBI:  NP_065393.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9NQC3

Overview

Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching.  Regulates neurite fasciculation, branching and extension in the developing nervous system.

Molecular Function

poly(A) RNA binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein

Expression

Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle.   Isoform 2 is widely expressed except for the liver.  Isoform 3 is expressed in brain, skeletal muscle and adipocytes.  Isoform 4 is testis-specific.

Structure

Binds to RTN4R, interacts with Bcl-xl and Bcl-2.  Isoform 2 binds to NGBR and RTN3.  Isoform 2 and Isoform 3 interact with BACE1 and BACE2, interacts with RTN4IP1 and ATL1.

Alternative Nomenclature

1110020G17Rik antibody

AA407876 antibody

AA409940 antibody

AA960376 antibody

ASY antibody

C130026I10Rik antibody

Foocen antibody

Glut4 vesicle 20 kDa protein antibody

Human NogoA antibody

Kiaa0886 antibody

KIAA4153 antibody

MGC116054 antibody

MGC139261 antibody

mKIAA0886 antibody

mKIAA4153 antibody

My043 protein antibody

Nbla00271 antibody

Nbla10545 antibody

Neurite growth inhibitor 220 antibody

Neuroendocrine-specific protein antibody

NI-250 antibody

Nogo A antibody

NOGO antibody

Nogo B antibody

Nogo C antibody

NSP antibody

NSP CL antibody

rat N antibody

Reticulon 4 antibody

Reticulon 5 antibody

RTN X antibody

RTN4 A antibody

RTN4-B1 antibody

Vp20 antibody

General Information

Product

PKC epsilon Antibody

Description

Affinity Purified Protein Kinase C epsilon Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human Protein Kinase C epsilon type protein.

Accession #

NCBI:  NP_005391.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q02156

Overview

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardio myocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. In HeLa cells, contributes to hepatocyte growth factor (HGF)-induced cell migration, and in human corneal epithelial cells, plays a critical role in wound healing after activation by HGF. During cytokinesis, forms a complex with YWHAP, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implication the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaciton with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a rolei n the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). invovled in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be invovled in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin-and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. IS able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). In prostate cancer cells, interacts with and phosphorylates STAT3, which increases DNA-binding and transcriptional activity of STAT3 and seems to be essential for prostate cancer cell invasion. Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines produciton. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm; Cell Membrane; Nucleus

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal

Contains 1 C2 domain.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Contains 1 protein kinase domain.

Alternative Nomenclature

KPCE_HUMAN antibody

MGC125656 antibody

MGC125657 antibody

nPKC epsilon antibody

nPKC-epsilon antibody

PKCE antibody

Pkcea antibody

PRKCE antibody

Protein kinase C epsilon antibody

General Information

Product

TYRO3 Antibody

Description

Affinity Purified Tyrosine-protein kinase receptor TYRO3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid reigon 800-850 on human Tyrosine-protein kinase receptor TYRO3 protein.

Accession #

NCBI:  NP_006284.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q06418

Overview

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including TULP1 or GAS6. Regulates many physiological processes including cell survival, migration and differentiation.

Molecular Function

Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane; Single-pass Type I membrane protein

Expression

Abundant in the brain and lower levels in other tissues.

Structure

Monomer and homodimer. Interacts (via N-terminus) with extracellular ligands TULP1 and GAS6. Interacts with PIK3R1; this interaction increases PI3-kinase activity.

Alternative Nomenclature

Brt 

BYK 

DTK 

RSE 

SKY 

Tif 

TYRO3 

Tyrosine-protein kinase byk 

Tyrosine-protein kinase DTK 

Tyrosine-protein kinase receptor TYRO3 

Tyrosine-protein kinase RSE 

Tyrosine-protein kinase SKY 

General Information

Product

TIPIN Antibody

Description

Affinity Purified Timeless-interacting protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 240-270 on mouse TIPIN protein.

Accession #

GenBank: DAA01365.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.96-0.98 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9BVW5

Overview

Plays an important role in the control of DNA replication and the maintenance of replication fork stability. Important for cell survival after DNA damage or replication stress.

Subcellular Location

Cytoplasm, Nucleus

Structure

Interacts with TIMELESS, which impairs TIMELESS self-association. Interacts with RPA2, PRDX2, MCM6 and MCM7.

Alternative Nomenclature

FLJ20516 antibody

TIMELESS interacting protein antibody

TIPIN antibody

General Information

Product

SFRP5 Antibody

Description

Affinity Purified Secreted frizzled-related protein 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on SFRP5

Accession #

GenBank: AAD25052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q5T4F7

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Molecular Function

PDZ domain binding, Wnt-protein binding, Wnt-activated receptor activity

Subcellular Location

Secreted

Expression

Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle.

Alternative Nomenclature

Frizzled related protein 1b

FRP 1b

SARP3

Secreted apoptosis related protein 3

Secreted frizzled related protein 5

General Information

Product

SFRP2 Antibody 

Description

Affinity Purified Secreted frizzled-related protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 225-295 on human SFRP2.

Accession #

GenBank: AAH08666.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96HF1

Overview

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Molecular Function

Endopeptidase activator activity, integrin binding, PDZ domain binding, Wnt-activated receptor activity, fibronectin binding, receptor agonist activity, Wnt-protein binding

Subcellular Location

Secreted

Expression

Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon.

Alternative Nomenclature

AI851596 antibody
Frizzled related protein 2 antibody
FRP2 antibody
SARP1 antibody
SDF5 antibody
Secreted apoptosis related protein 1 antibody
Secreted frizzled-related protein 2 antibody

General Information

Product

SIRT3 Antibody

Description

Affinity Purified NAD-dependent protein deacetylase sirtuin-3, mitochondrial Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide:

RDLVGPFVLSPRRKDVVQL

corresponding to amino acids 203-221 of rat SIRT3 protein.

Accession #

NCBI:  NP_001099783

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B2RZ31

Molecular Function

enzyme binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K14 specific)

Alternative Nomenclature

Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody

NAD dependent deacetylase sirtuin 3 mitochondrial antibody

Regulatory protein SIR2 homolog 3 antibody

Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody

SIR 2 like protein 3 antibody

SIR 3 antibody

SIR2 L3 antibody

Sirtuin 3 antibody

Sirtuin type 3 antibody

General Information

Product

SIRT7 Antibody

Description

Affinity Purified Sirtuin (silent mating type information regulation 2 homolog) 7 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on mouse SIRT7 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8BKJ9

Overview

NAD-dependent protein deacetylase that specifially mediates deacetylation of histone H3 at 'Lys-18'. Displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression.

Molecular Function

Chromatin binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K18 specific), metal ion binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: Located close to the nuclear membrane when in cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

Expression

Detected in liver, spleen and testis. Detected in embryos.

Structure

Belongs to the Sirtuin Family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 7 antibody

Regulatory protein SIR2 homolog 7 antibody

Silent mating type information regulation 2 homolog antibody

SIR2 L7 antibody

SIR2 like protein 7 antibody

Sir2 related protein type 7 antibody

SIRT7 antibody

Sirtuin 7 antibody

Sirtuin type 7 antibody