General Information

Product

IFI16 Antibody Affinity Purified

Description

Affinity Purified Gamma-interferon-inducible protein 16 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 140-190 on human IFI16 protein

Accession #

NCBI:  NP_001193496.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q16666

Overview

Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein.

Molecular Function

Core promoter binding, identical protein binding, transcription factor binding, double-standard DNA binding, poly(A) RNA binding

Subcellular Location

Nucleus, cytoplasm (Note: Cellular distribution is dependent on acetylation status of multipartite nuclear localization signal(NLS); NLS acetylation promotes cytoplasmic localization. Localizes in nucleus during human herbes simplex virus 1 infection)

Expression

Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prosta

Alternative Nomenclature

IFI16 antibody

Gamma interferon inducible protein 16 antibody

Ifi204 antibody

IFNGIP1 antibody

interferon activated gene 204 antibody

Interferon gamma inducible protein 16 antibody

p204 antibody

PYHIN2 antibody

General Information

Product

mTOR Antibody Affinity Purified

Description

Affinity Purified Serine/threonine-protein kinase mTOR Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 430-480 on mTOR protein.

Accession #

NCBI:  NP_063971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P42346

Overview

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Lysosome, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Alternative Nomenclature

FK506 binding protein 12 rapamycin associated protein 1 antibody

FKBP rapamycin associated protein antibody

FRAP1 antibody

FRAP2 antibody

mTOR antibody

RAFT1 antibody

Rapamycin target protein antibody

RAPT1 antibody

Serine/threonine-protein kinase mTOR antibody

General Information

Product

Phospho-ACK1 Antibody Antibodyd

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on ACK1 protein. 

Accession #

GenBank: AAL55732.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P13749

Overview

Plays a role in presenting foreign antigens to the immune system.

Molecular Function

peptide antigen bonding

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer of an alpha chain and a beta chain

Alternative Nomenclature

Acetate kinase 1 antibody

ACK 1 antibody

ACK antibody

Activated Cdc42 associated kinase 1 antibody

Activated p21cdc42Hs kinase antibody

FLJ44758 antibody

FLJ45547 antibody

p21cdc42Hs antibody

TNK 2 antibody

Tyrosine kinase non receptor 2 antibody

General Information

Product

Quiescine Q6 Antibody

Description

Affinity Purified Sulfhydryl oxidase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-550 on human QSOX1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O00391

Overview

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.  May contribute to disulfide bond formation in a variety of secreted proteins.

Molecular Function

Oxidoreductase, flavin-linked sulfhydryl oxidase activity, protein disulfide isomerase activity

Subcellular Location

Isoform 1: Golgi apparatus membrane; Single plass membrane protein Isoform 2: Secreted > extracellular space

Expression

Expressed in heart, placenta, lung, liver, skeletal muscle, pancreas and very weakly in brain and kidney.

Structure

Monomer

Alternative Nomenclature

FLJ34858 antibody

hQSOX antibody

Human quiescin (Q6) mRNA, partial cds antibody

Q6 antibody

QSCN6 antibody

QSOX1 antibody

Quiescin Q6 antibody

Quiescin Q6 sulfhydryl oxidase 1 antibody

Skin sulfhydryl oxidase antibody

Sox antibody

Sulfhydryl oxidase 1 antibody

General Information

Product

ROCK1 Antibody

Description

Affinity Purified Rho-associated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region spanning 850-900 on human ROCK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13464

Overview

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Golgi apparatus membrane; Peripheral membrane protein, Cell projection

Expression

Detected in blood platelets.

Structure

Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

Alternative Nomenclature

coiled-coil-containing protein kinase 1 antibody

NY REN 35 antigen antibody

p160 Rhoassociated coiled coil-forming protein kinase antibody

p160 ROCK1 antibody

PRO0435 antibody

Renal carcinoma antigen NY REN 35 antibody

Rho associated coiled coil containing protein kinase 1 antibody

Rho-alpha kinase antibody

Rho-associated protein kinase 1 antibody

Rok antibody

rokalpha antibody

General Information

Product

p63 Antibody

Description

Affinity Purified Tumor protein 63 isoform 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human p63 protein.

Accession #

NCBI:  NP_001108454

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9H3D4

Overview

Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes.

Molecular Function

Activator, Developmental protein, chromatin binding

Subcellular Location

Nucleus

Expression

Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. 

Structure

Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L.

Alternative Nomenclature

AIS antibody

Amplified in squamous cell carcinoma antibody

B(p51A) antibody

B(p51B) antibody

Chronic ulcerative stomatitis protein antibody

CUSP antibody

DN p63 alpha 1 antibody

EEC3 antibody

Keratinocyte transcription factor antibody

KET antibody

LMS antibody

MGC115972 antibody

MGC192897 antibody

NBP antibody

OFC8 antibody

p40 antibody

p51 antibody

P51/P63 antibody

p53CP antibody

p63 antibody

P73 antibody

p73H antibody

p73L antibody

RHS antibody

SHFM4 antibody

TAp63alpha antibody

TP53CP antibody

TP53L antibody

TP63 antibody

TP73 antibody

Transformation related protein 63 antibody

Trp53rp1 antibody

Trp63 antibody

Tumor protein 63 antibody

Tumor protein p53-competing protein antibody

Tumor protein p63 deltaN isoform delta antibody

Tumor protein p73 antibody

General Information

Product

PELO Antibody

Description

Affinity Purified PELOTA Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-380 on human PELOTA protein.

Accession #

GenBank: AAG22575.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9BRX2

Overview

Is necessary in cell division for normal chromosome segregation and genomic stability.  It may recognize stalled ribosomes and trigger endonucleolytic cleavage of the mRNA, and may have ribonuclease activity.

Molecular Function

Endonuclease, Hydrolase, Nuclease

Subcellular Location

Nuclease, Cytoplasm

Expression

Expressed ubiquitously

Structure

The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity (Potential).  Belongs to the eukaryotic release factor 1 family. Pelota subfamily.

Alternative Nomenclature

CGI 17 antibody

pelo antibody

pelota (Drosophila) homolog antibody

pelota homolog antibody

PRO1770 antibody

Protein pelota homolog antibody

General Information

Product

Cyclin D1 Antibody

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 1-50 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody Mid-Region

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 10-60 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 170-220 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody