General Information

Product

Cyclin D1 Antibody

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to amino acid sequences 213-232 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody Mid-Region

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acid sequences 268-295 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to amino acid sequences 280-295 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

p27 KIP 1 Antibody

Description

Affinity Purified Cyclin-dependent kinase inhibitor p27kip1 Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on human p27 KIP1 protein.

Accession #

GenBank: AAF69497.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:250

Protein

Uniprot #

Q9NYG6

Overview

cyclin-dependent protein serine/threonine kinase inhibitor activity;plays a role in cell cycle arrest

Molecular Function

Cyclin;Kinase;Transferase

Subcellular Location

Nucleus

Alternative Nomenclature

AA408329 antibody

AI843786 antibody

CDKN 1B antibody

CDKN 4 antibody

Cyclin dependent kinase inhibitor p27 antibody

KIP 1 antibody

MEN1B antibody

MEN4 antibody

OTTHUMP00000195098 antibody

p27 antibody

General Information

Product

CDKKN2A / p14ARF Antibody 

Description

Affinity Purified partial tumor supressor p14ARF Antibody Mid-Region

Verified Applications

ELISA, WB

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Mid-Region epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein

Accession #

GenBank: AAC6069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 mg/ml µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N726

Overview

Functions as a tumor supressor and has the ability to induce cell cycle arrest in the G1 and G2 phases.  Serves to induce G2 arrest and apoptosis in a p53-independent manner via preventing the activation of cyclin B1/CDC2 complexes.

Molecular Function

DNA binding, RNA binding, p53 binding, MDM2/MDM4 family protein binding

Subcellular Location

Nucleus

Structure

No interaction with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6.  Interacts with TBRG1, COMMD1, CDKN2AIP and E4F1.  Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9.

Alternative Nomenclature

ARF antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN 2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

1/2/3 antibody

Cyclin dependent kinase inhibitor p16 antibody

INK4 antibody

Melanoma p16 inhibits CDK4 antibody

MLM antibody

MTS 1 antibody

p14 antibody

p16 antibody

p19 antibody

P19ARF antibody

TP16 antibody

General Information

Product

CDKKN2A / p14ARF Antibody

Description

Affinity Purified partial tumor supressor p14ARF Antibody C-epitope

Verified Applications

ELISA, WB

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to C-terminal amino acids 94-111 of Human p14ARF.

Sequence:

QRLGQQPLPRRPGHDDGQ

Accession #

GenBank: AAC6069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 mg/ml µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N726

Overview

Functions as a tumor supressor and has the ability to induce cell cycle arrest in the G1 and G2 phases.  Serves to induce G2 arrest and apoptosis in a p53-independent manner via preventing the activation of cyclin B1/CDC2 complexes.

Molecular Function

DNA binding, RNA binding, p53 binding, MDM2/MDM4 family protein binding

Subcellular Location

Nucleus

Structure

No interaction with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6.  Interacts with TBRG1, COMMD1, CDKN2AIP and E4F1.  Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9.

Alternative Nomenclature

ARF antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN 2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

1/2/3 antibody

Cyclin dependent kinase inhibitor p16 antibody

INK4 antibody

Melanoma p16 inhibits CDK4 antibody

MLM antibody

MTS 1 antibody

p14 antibody

p16 antibody

p19 antibody

P19ARF antibody

TP16 antibody

General Information

Product

CDKN2A / p14ARF Antibody

Description

Affinity Purified partial tumor supressor p14ARF Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein

Accession #

GenBank: AAC6069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 mg/ml µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N726

Overview

Functions as a tumor supressor and has the ability to induce cell cycle arrest in the G1 and G2 phases.  Serves to induce G2 arrest and apoptosis in a p53-independent manner via preventing the activation of cyclin B1/CDC2 complexes.

Molecular Function

DNA binding, RNA binding, p53 binding, MDM2/MDM4 family protein binding

Subcellular Location

Nucleus

Structure

No interaction with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6.  Interacts with TBRG1, COMMD1, CDKN2AIP and E4F1.  Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9.

Alternative Nomenclature

ARF antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN 2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

1/2/3 antibody

Cyclin dependent kinase inhibitor p16 antibody

INK4 antibody

Melanoma p16 inhibits CDK4 antibody

MLM antibody

MTS 1 antibody

p14 antibody

p16 antibody

p19 antibody

P19ARF antibody

TP16 antibody

General Information

Product

Phospho-p27 KIP1 Antibody

Description

Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody Extreme C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 148-198 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.

Accession #

GenBank: AAH01971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:250

Protein

Uniprot #

Q9NYG6

Overview

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Molecular Function

Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity

Subcellular Location

Nucleus

Expression

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Structure

Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex.

Alternative Nomenclature

AA408329 antibody

AI843786 antibody

CDKN 1B antibody

CDKN 4 antibody

Cyclin Dependent Kinase Inhibitor 1B antibody

Cyclin dependent kinase inhibitor p27 antibody

KIP 1 antibody

MEN1B antibody

MEN4 antibody

p27 antibody

p27 Kip1 antibody

P27-like cyclin-dependent kinase inhibitor antibody

General Information

Product

Phospho-p27 KIP1 Antibody

Description

Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 143-193 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.

Accession #

GenBank: AAH01971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:250

Protein

Uniprot #

Q9NYG6

Overview

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Molecular Function

Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity

Subcellular Location

Nucleus

Expression

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Structure

Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex.

Alternative Nomenclature

AA408329 antibody

AI843786 antibody

CDKN 1B antibody

CDKN 4 antibody

Cyclin Dependent Kinase Inhibitor 1B antibody

Cyclin dependent kinase inhibitor p27 antibody

KIP 1 antibody

MEN1B antibody

MEN4 antibody

p27 antibody

p27 Kip1 antibody

P27-like cyclin-dependent kinase inhibitor antibody

General Information

Product

LYK5 Antibody

Description

Affinity Purified STE20-related kinase adapter protein alpha Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 381-431 on human STE-20 related kinase adapter protein alpha.

Accession #

NCBI:  NP_001003787.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q7RTN6

Overview

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Molecular Function

ATP Binding, protein kinase activator activity, kinase binding, transferase activity, transferring phosphorus-containing groups

Subcellular Location

Nucleus, cytoplasm

Expression

Expressed in liver

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 protein kinase domain.

Alternative Nomenclature

Protein kinase LYK5 antibody

Serologically defined breast cancer antigen NY BR 96 antibody

STE20 related adapter protein antibody

STRAD alpha antibody

STRAD antibody