General Information

Product

TOMM5 Antibody

Description

Affinity Purified Mitochondrial import receptor subunit TOM5 homolog Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Mitochondrial import receptor subunit TOM5 homolog isoform 1 protein.

Accession #

NCBI:  NP_001001790.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q8N4H5

Overview

Cellular protein metabolic process; Protein targeting to mitochondrion

Molecular Function

Protein Transport

Subcellular Location

Membrane, Mitochondrion, Mitochondrion outer membrane

Structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70).

Alternative Nomenclature

1110019J04Rik antibody

C9orf105 antibody

Mitochondrial import receptor subunit TOM5 homolog antibody

RP11-263I4.1 antibody

RP11-613M10.3 antibody

TOM5 antibody

Translocase of outer mitochondrial membrane 5 homolog (yeast) antibody

General Information

Product

TOMM6 Antibody

Description

Affinity Purified Mitochondrial import receptor subunit TOM6 homolog Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 24-74 on human Mitochondrial import receptor subunit TOM6 homolog.

Accession #

NCBI:  NP_001127965.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96B49

Overview

Cellular protein metabolic process; Protein targeting to mitochondrion

Molecular Function

Protein Transport

Subcellular Location

Membrane, Mitochondrion, Mitochondrion outer membrane

Structure

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70).

Alternative Nomenclature

Mitochondrial import receptor subunit TOM6 homolog antibody
TOM6 antibody

General Information

Product

ORP150 Antibody

Description

Affinity Purified Hypoxia up regulated 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Synthetic peptide taken within amino acid region 950-999 on human Hypoxia up regulated 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y4L1

Overview

Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.

Molecular Function

ATP binding; chaperone binding

Subcellular Location

Endoplasmic reticulum lumen

Expression

Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.

Structure

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the heat shock protein 70 family.

Alternative Nomenclature

150 kDa oxygen regulated protein antibody

170 kDa glucose regulated protein antibody

DKFZp686N08236 antibody

FLJ94899 antibody

FLJ97572 antibody

glucose regulated protein 170 antibody

Grp 170 antibody

Grp170 antibody

HSP 12A antibody

HSP12A antibody

Hyou1 antibody

Hypoxia up regulated 1 antibody

Orp 150 antibody

Orp150 antibody

oxygen regulated protein (150kD) antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 20-70 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Structure

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 80-130 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Expression

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody Mixed Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 50-100 and 80-130 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multiple Epitopes

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Expression

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

SPACA3 Antibody

Description

Affinity Purified Sperm acrosome membrane-associated protein 3 Antibody Mid-region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 50-100 on human sperm acrosome membrane-associated protein 3 isoform 2.

Specificity

This antibody will label all isoforms of sperm acrosome membrane-associated protein 3.

Accession #

Gen Bank: AF099029.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8IXA5

Overview

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane.

Molecular Function

lysozyme activity

Subcellular Location

cytoplasmic vesicle, single-pass type II membrane protein

Expression

The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.

Alternative Nomenclature

ALLP17 antibody

Cancer/testis antigen 54 antibody

CT54 antibody

LYC3 antibody

Lysozyme like sperm specific secretory protein ALLP17 antibody

Lysozyme-like acrosomal sperm-specific secretory protein ALLP-17 antibody

Lysozyme-like protein 3 antibody

LYZL3 antibody

processed form antibody

SLLP1 antibody

SPACA3 antibody

Sperm acrosome associated 3 antibody

Sperm acrosome membrane-associated protein 3 antibody

Sperm lysozyme-like protein 1 antibody

Sperm protein reactive with antisperm antibodies antibody

Sperm protein reactive with ASA antibody

General Information

Product

OGT1 Antibody

Description

Affinity Purified O-linked N-acetylglucosamine transferase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 980-1030 on human O-linked N-acetylglucosamine transferase protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O15294

Overview

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity.

Molecular Function

Chromatin regulator, Glycosyltransferase, Transferase

Subcellular Location

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus

Expression

Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

Structure

The TPR repeat domain is required for substrate binding and oligomerization.

Alternative Nomenclature

GlcNAc transferase

HRNT1 

O GlcNAc 

O GlcNAc transferase p110 subunit 

O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) 

UDP N acetylglucosamine peptide N

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit