General Information

Product

TSG101 Antibody

Description

Affinity Purified Tumor susceptibility gene 101 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-390 on Tumor susceptibility gene 101 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q99816

Overview

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Molecular Function

calcium-dependent protein binding

DNA binding

ligand-dependent nuclear receptor transcription coactivator activity

protein homodimerization activity

transcription corepressor activity

ubiquitin binding

ubiquitin protein ligase binding

virion binding

Subcellular Location

Cytoplasm

Membrane; Peripheral membrane protein

Nucleus

Late endosome membrane; Peripheral membrane protein

Expression

Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.

Alternative Nomenclature

ESCRT I complex subunit TSG101 antibody

TS101_HUMAN antibody

TSG 10 antibody

TSG 101 antibody

Tumor susceptibility gene 10 antibody

Tumor susceptibility gene 101 antibody

Tumor susceptibility protein antibody

Tumor susceptibility protein isoform 3 antibody

VPS23 antibody

General Information

Product

HIPK4 Antibody

Description

Affinity Purified Homeodomain-interacting protein kinase 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 470-550 on human Homeodomain-interacting protein kinase 4.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q8NE63

Overview

Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).

Molecular Function

ATP binding

protein serine/threonine kinase activity

Subcellular Location

Cytoplasm, nucleus

Structure

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.

Alternative Nomenclature

HIPK 4 antibody

Homeodomain interacting protein kinase 4 antibody

General Information

Product

HIPK2 Antibody

Description

Affinity Purified Homeodomain-interacting protein kinase 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1150-1198 on human Homeodomain-interacting protein kinase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9H2X6

Overview

Serine/threonine-protein kinase involved in transcription regulation, p53/TP53-mediated cellular apoptosis and regulation of the cell cycle. Acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors. Phosphorylates PDX1, ATF1, PML, p53/TP53, CREB1, CTBP1, CBX4, RUNX1, EP300, CTNNB1, HMGA1 and ZBTB4. Inhibits cell growth and promotes apoptosis through the activation of p53/TP53 both at the transcription level and at the protein level (by phosphorylation and indirect acetylation). The phosphorylation of p53/TP53 may be mediated by a p53/TP53-HIPK2-AXIN1 complex. Involved in the response to hypoxia by acting as a transcriptional co-suppressor of HIF1A.

Molecular Function

ATP binding

protein kinase activity

protein serine/threonine kinase activity

RNA polymerase II activating transcription factor binding

RNA polymerase II transcription coactivator activity

SMAD binding

transcription corepressor activity

virion binding

Subcellular Location

Nucleus, PML body, Cytoplasm

Expression

Highly expressed in heart, muscle and kidney. Weakly expressed in a ubiquitous way. Down-regulated in several thyroid and breast tumors.

Alternative Nomenclature

HIPK 2 antibody

Homeodomain interacting protein kinase 2 antibody

Nbak1 antibody

Nuclear body-associated kinase 1 antibody

PRO0593 antibody

Sialophorin tail-associated nuclear serine/threonine-protein kinase antibody

Stank antibody

General Information

Product

PARD6B Antibody

Description

Affinity Purified Partitioning Defective 6 homolog beta Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 300-350 on human Partitioning Defective 6 homolog beta.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9BYG5

Overview

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Molecular Function

protein kinase C binding

Rho GTPase binding

Subcellular Location

Cell membrane, Cytoplasm

Expression

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Structure

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Alternative Nomenclature

OTTHUMP00000031269 antibody

PAR 6 beta antibody

Par 6 partitioning defective 6 homolog beta (C. elegans) antibody

PAR6B antibody

PARD6 beta antibody

PARD6B antibody

partitioning defective 6 homolog beta antibody

partitioning-defective protein 6, C. elegans, homolog of, beta antibody

General Information

Product

PARD6B Antibody

Description

Affinity Purified Partitioning Defective 6 homolog beta Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 340- 372 on human Partitioning Defective 6 homolog beta.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9BYG5

Overview

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Molecular Function

protein kinase C binding

Rho GTPase binding

Subcellular Location

Cell membrane, Cytoplasm

Expression

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Structure

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Alternative Nomenclature

OTTHUMP00000031269 antibody

PAR 6 beta antibody

Par 6 partitioning defective 6 homolog beta (C. elegans) antibody

PAR6B antibody

PARD6 beta antibody

PARD6B antibody

partitioning defective 6 homolog beta antibody

partitioning-defective protein 6, C. elegans, homolog of, beta antibody

General Information

Product

EB2 Antibody

Description

Microtubule-associated protein RP/EB family member Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Zebra Fish

Immunogen

Synthetic peptide corresponding to amino acids within region 1-50 on zebra fish Microtubule-associated protein RP/EB family member protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Unconjugated

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

A7MC00

Overview

microtubule binding

microtubule plus-end binding

Subcellular Location

cytoskeleton

Alternative Nomenclature

APC binding protein EB1 antibody

APC binding protein EB2 antibody

EB1 antibody

EB2 antibody

End binding protein 2 antibody

H.sapiens mRNA for novel T cell activation protein antibody

MAPRE2 antibody

MARE2_HUMAN antibody

Microtubule associated protein RP/EB family member 2 antibody

RP1 antibody

T cell activation protein EB1 family antibody

General Information

Product

EB2 Antibody

Description

Affinity Purified Microtubule-associated protein RP/EB family member Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Zebra Fish

Immunogen

Synthetic peptide corresponding to amino acids within region 150-210 on zebra fish Microtubule-associated protein RP/EB family member protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

A7MC00

Overview

microtubule binding

microtubule plus-end binding

Subcellular Location

cytoskeleton

Alternative Nomenclature

APC binding protein EB1 antibody

APC binding protein EB2 antibody

APC-binding protein EB2 antibody

EB1 antibody

EB2 antibody

End binding protein 2 antibody

End-binding protein 2 antibody

H.sapiens mRNA for novel T cell activation protein antibody

MAPRE2 antibody

MARE2_HUMAN antibody

Microtubule associated protein RP/EB family member 2 antibody

Microtubule-associated protein RP/EB family member 2 antibody

RP1 antibody

T cell activation protein EB1 family antibody