General Information

Product

ACK1 Antibody

Description

Affinity Purified Activated CDC42 kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on ACK1 protein.

Accession #

GenBank: L13738.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q07912

Overview

Non-receptor tyrosine-protein and serine/threonine-protein kinase implicated in cell sperading and migration, survival, growth and proliferation.  Transduces extracellular signals to cytosolic and nuclear effectors.

Molecular Function

ATP binding; epidermal growth factor receptor binding; GTPase inhibitor activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine kinase activity

Subcellular Location

Cell membrane; Nucleus; Endosome; Cell junction; adherens junction; Cytoplasmic vessicle membrane; Peripheral membrane protein; Clathrin-coated vessicles; Clathin coated pit

Expression

During progressive stages in breast and prostate cancer

Structure

Forms  complexes with GRB2 and several receptor tyrosine kinases.  Part of collagen stimulated complex.

Alternative Nomenclature

Acetate kinase 1 antibody

Acetokinase 1 antibody

ACK 1 antibody

Activated Cdc42 associated kinase 1 antibody

Activated CDC42 kinase 1 antibody

Activated p21cdc42Hs kinase antibody

p21cdc42Hs antibody

TNK 2 antibody

Tyrosine kinase non-receptor protein 2 antibody

General Information

Product

GADD34 Antibody

Description

Affinity Purified Growth arrest and DNA damage-inducible protein GADD34 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Predicted: Bovine, Monkey

Immunogen

Synthetic peptide corresponding to internal sequence of amino acids 380-400 of Human GADD34, covalently modified and coupled to a carrier protein.

Sequence:

PATGVFLKSWVYQPGEDTEEE

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:800-1:1,000

ELISA

1:800-1:1,000

Western Blot

1:500

Protein

Uniprot #

O75807

Overview

Recruits serine/threonine-protein phosphatase PP1 to dephosphorylate translation initation factor eIF2A/EIF2S1.  This reverses shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress.

Molecular Function

Apoptosis; Stress Response; Translation regulation

Subcellular Location

Endoplasmic Reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic Side

Expression

induced by methyl methanesulfonate and ionizing irradiation.  Apoptosis is induced by interluekin 24 in melanoma cells

Structure

belongs to PPP1R15 family.

Alternative Nomenclature

Apoptosis associated protein antibody

GADD 34 antibody

Growth arrest and DNA-damage-inducible 34 antibody

Myeloid differentiation primary response protein MyD116 homolog antibody

PPP1R15A antibody

Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody

General Information

Product

Phospho-ACK1 Antibody Antibodyd

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on ACK1 protein. 

Accession #

GenBank: AAL55732.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-1.25 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P13749

Overview

Plays a role in presenting foreign antigens to the immune system.

Molecular Function

peptide antigen bonding

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer of an alpha chain and a beta chain

Alternative Nomenclature

Acetate kinase 1 antibody

ACK 1 antibody

ACK antibody

Activated Cdc42 associated kinase 1 antibody

Activated p21cdc42Hs kinase antibody

FLJ44758 antibody

FLJ45547 antibody

p21cdc42Hs antibody

TNK 2 antibody

Tyrosine kinase non receptor 2 antibody

General Information

Product

PTPD1 Antibody 

Description

Affinity Purified PTPN21 protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-500 on mouse PTPND1 protein.

Accession #

GenBank: AAH98500.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.87 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:250

Protein

Uniprot #

Q4KML9

Overview

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Molecular Function

Hydrolase, Protein Phosphatase, Protein tyrosine Phosphatase activity

Subcellular Location

Cytoskeleton

Alternative Nomenclature

protein tyrosine phosphatase D1 antibody

Protein tyrosine phosphatase non receptor type 21 antibody

PTPNL antibody

PTPRL10 antibody

General Information

Product

Slingshot homolog 1 Antibody Affinity Purified

Description

Affinity Purified Protein phosphatase Slingshot homolog 1 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Slingshot homolog 1 protein.

Accession #

NCBI:  NP_061857.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8WYL5

Overview

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.

Molecular Function

Hydrolase, protein phosphatase

Subcellular Location

Cytoplasm, cytoskeleton, cell projection, lamellipodium, cleavage furrow, midbody. (Note: Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at site of cell locomotion. Also localized to

Alternative Nomenclature

AW551225 antibody

Coro1c antibody

coronin, actin binding protein 1C antibody

Gm1394 antibody

Gm1395 antibody

Protein phosphatase Slingshot homolog 1 antibody

slingshot like 1 antibody

SSH1 antibody

General Information

Product

RIPK1 Antibody Affinity Purified

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 1

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within aino acid region 620-670 on human Receptor-interacting Serine/Threonine-protein kinase 1 protein.

Accession #

NCBI:  NP_003795.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13546

Overview

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Molecular Function

ATP binding; Death domain binding; Identical protein binding; Protein complex binding; Protein kinase activity; protein serine/threonine kinase activity; Ubiquitin protein ligase binding

Subcellular Location

Cytoplasm

Structure

Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif). Upon TNF-induced necrosis, the RIPK1-RIPK3 dimer further interacts with PGAM5 and MLKL; the formation of this complex leads to PGAM5 phosphorylation and increase in PGAM5 phosphatase activity. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1 (By similarity). Interacts with ZBP1 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK3 and MLKL. Interacts (via kinase domain) with DAB2IP (via Ras-GAP domain); the interaction occurs in a TNF-alpha-dependent manner. Interacts with ARHGEF2. Interacts (via protein kinase domain) with RFFL; involved in RIPK1 ubiquitination. Interacts with RNF34; involved in RIPK1 ubiquitination.

Alternative Nomenclature

Cell death protein RIP antibody

Receptor (TNFRSF) interacting serine threonine kinase 1 antibody

Receptor-interacting protein 1 antibody

Serine threonine protein kinase RIP antibody

General Information

Product

Phospho Slingshot homolog 1 Antibody

Description

Affinity Purified Phosphorylated Protein phosphatase Slingshot homolog 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequence on Slingshot homolog 1 protein.

Accession #

NCBI:  NP_061857.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8WYL5

Overview

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.

Molecular Function

Hydrolase, protein phosphatase

Subcellular Location

Cytoplasm, cytoskeleton, cell projection, lamellipodium, cleavage furrow, midbody. (Note: Also recruited to actin rich membrane protrusions such as lamellipodia, which may allow local control of actin dynamics at site of cell locomotion.

Alternative Nomenclature

AW551225 antibody

Coro1c antibody

coronin, actin binding protein 1C antibody

Gm1394 antibody

Gm1395 antibody

Protein phosphatase Slingshot homolog 1 antibody

slingshot like 1 antibody

SSH1 antibody

General Information

Product

LYK5 Antibody

Description

Affinity Purified STE20-related kinase adapter protein alpha Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 381-431 on human STE-20 related kinase adapter protein alpha.

Accession #

NCBI:  NP_001003787.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q7RTN6

Overview

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Molecular Function

ATP Binding, protein kinase activator activity, kinase binding, transferase activity, transferring phosphorus-containing groups

Subcellular Location

Nucleus, cytoplasm

Expression

Expressed in liver

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 protein kinase domain.

Alternative Nomenclature

Protein kinase LYK5 antibody

Serologically defined breast cancer antigen NY BR 96 antibody

STE20 related adapter protein antibody

STRAD alpha antibody

STRAD antibody

General Information

Product

LYK5 Antibody

Description

Affinity Purified STE20-related kinase adapter protein alpha Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human STE-20 related kinase adapter protein alpha.

Accession #

NCBI:  NP_001003787.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q7RTN6

Overview

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Molecular Function

ATP Binding, protein kinase activator activity, kinase binding, transferase activity, transferring phosphorus-containing groups

Subcellular Location

Nucleus, cytoplasm

Expression

Expressed in liver

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 protein kinase domain.

Alternative Nomenclature

Protein kinase LYK5 antibody

Serologically defined breast cancer antigen NY BR 96 antibody

STE20 related adapter protein antibody

STRAD alpha antibody

STRAD antibody

General Information

Product

DCK Antibody

Description

Affinity Purified Deoxycytidine kinase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 210-260 on mouse Deoxycytidine kinase.

Accession #

NCBI:  NP_031858.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P43346

Overview

Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA).

Molecular Function

ATP binding; deoxycytidine kinase activity; drug binding; nucleoside kinase activity; protein homodimerization activity

Subcellular Location

Nucleus

Structure

Homodimer; Belongs to the DCK/DGK family.

Alternative Nomenclature

DCK protein antibody

Deoxycytidine kinase antibody

Deoxynucleoside kinase antibody

EC 2.7.1 antibody

EC 2.7.1.74 antibody

MGC117410 antibody

MGC138632 antibody