General Information

Product

TLR10 Antibody

Description

Affinity Purified Toll-like receptor 10 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chimpanzee, Human, Monkey, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region 380-430 on human TLR10 protein.

Accession #

GenBank: AAQ88667.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.67 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9BXR5

Overview

Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Molecular Function

Receptor, transmembrane signaling receptor activity

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Highly expressed in spleen, lymph node, thymus, tonsil and at lower levels in lung. Highly expressed in promyelocytic HL-60 cells and in B-cell lines.

Structure

Binds MYD88 via their respective TIR domains (By similarity). Homodimer (Potential).

Alternative Nomenclature

CD 290 antibody

CD 290 antigen antibody

TLR10 antibody

Toll like receptor 10 antibody

UNQ315/PRO358 antibody

General Information

Product

TLR11 Antibody Affinity Purified

Description

Affinity Purified Toll-like receptor 11 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Chimpanzee, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 430-460 on human TLR11 protein.

Accession #

NCBI:  NP_991388.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q6R5P0

Overview

Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Molecular Function

Receptor, transmembrane signaling receptor activity

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Binds MYD88 via their respective TIR domains (By similarity). Interacts with HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum.

Alternative Nomenclature

hgnc31753 antibody

Toll like receptor 11 antibody

General Information

Product

TLR12 Antibody Affinity Purified

Description

Affinity Purified Toll-like receptor 12 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Chimpanzee, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 440-470 from mouse TLR12 protein.

Accession #

NCBI:  NP_991392.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q6QNU9

Overview

Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Molecular Function

Receptor

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Macrophages, liver, kidney and bladder epithelial cells.

Structure

Binds MYD88 via their respective TIR domains.

Alternative Nomenclature

Toll-like receptor 12

General Information

Product

TLR13 Antibody

Description

Affinity Purified Toll-like receptor 13 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-480 of mouse TLR13 protein.

Accession #

GenBank: AAI17914.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q6R5N8

Overview

Component of innate and adaptive immunity that recognizes and binds 23S rRNA from bacteria. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Molecular Function

protein homodimerization activity, rRNA binding

Subcellular Location

endosome membrane, single-pass type I membrane protein

Alternative Nomenclature

Toll like receptor 13 antibody

General Information

Product

TRAF6 Antibody Affinity Purified

Description

Affinity Purified TNF receptor-associated factor 6 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on TRAF6 protein.

Accession #

NCBI:  NP_665802.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9Y4K3

Overview

Mediates synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, may be essential for formation of functional osteoclasts, seems to also play a role in dendritic cells maturation and/or activation.

Molecular Function

histone deacetylase binding, ligase activity, zinc ion binding, protein kindase B binding, protein N-terminus binding signal transducer activity

Subcellular Location

Cytoplasm, cell cortex, nucleus, lipid droplet. (Note: Found in nuclei of some aggressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T- and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitinat

Expression

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Alternative Nomenclature

E3 ubiquitin-protein ligase TRAF6 antibody

Interleukin 1 signal transducer antibody

RING finger protein 85 antibody

RNF85 antibody

TNF receptor-associated factor 6, E3 ubiquitin protein ligase antibody

General Information

Product

TRIF Antibody 

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on TRIF protein.

Accession #

GenBank:  BAA76376.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q9CQE0

Overview

E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway (By similarity).

Molecular Function

Ligase, Zinc ion binding

Subcellular Location

Nucleus

Structure

Interacts with NLK (via C-terminus).

Alternative Nomenclature

MGC35334 antibody

Proline rich vinculin and TIR domain containing protein B antibody

Proline-rich antibody

PRVTIRB antibody

Putative NF kappa B activating protein 502H antibody

Putative NFkB activating protein antibody

TICAM 1 antibody

TIR domain-containing adapter protein inducing IFN-beta antibody

Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody

Toll like receptor adaptor molecule 1 antibody

Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody

TRIF antibodyvinculin and TIR domain-containing protein B antibody

General Information

Product

Phospho-BTK Antibody (Y551)

Description

Affinity Purified Phospho-specific Bruton's Tyrosine Kinase Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide corresponding to Human BTK amino acids 544-561 (phopsho Y551). 

Sequence 

RYV LDD EY(p)T SSV GSK FPV

Accession #

NCBI:  NP_000052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500. Predicted molecular weight: 76 kDa.

Protein

Uniprot #

Q06187

Overview

Non-receptor tyrosine kinase invovled in B lymphocyte development.

Molecular Function

Kinase; Transferase; Tyrosine-protein Kinase

Subcellular Location

Cytoplasm; Cell Membrane; Peripheral membrane: Nucleus.  In steady state BTK is predominantly cytosolic.

Expression

B-lymphocytes

Structure

PH domain mediates binding to inositol polyphosphate and phosphoinositides, leading to its targeting with plasma membrane.  Extended in the BTK kinase family by a region designated Tec homology domain, which has roughly 80 residues preceding SH3 domain.

Alternative Nomenclature

Agammaglobulinaemia tyrosine kinase antibody

AGMX 1 antibody

AT antibody

ATK antibody

B cell progenitor kinase antibody

BPK antibody

Bruton tyrosine kinase antibody

Btk antibody

IMD1 antibody

MGC126261 antibody

MGC126262 antibody

PSCTK 1 antibody

Tyrosine protein kinase BTK antibody

XLA antibody

General Information

Product

RIPK2 Antibody Affinity Purified

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 2 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Pig, Monkey

Immunogen

Synthetic peptide taken within amino acid region 410-460 on human RIPK2 protein.

Accession #

GenBank: AAQ89173.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O43353

Overview

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Molecular Function

ATP binding; CARD domain binding; LIM domain binding; non-membrane spanning protein tyrosine kinase activity; Protein homodimerization activity; protein serine/threonine kinase activity; signal transducer activity

Subcellular Location

Cytoplasm

Expression

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Structure

Found in a signaling complex consisting of at least ARHGEF2, NOD2 and RIPK2. Interacts with ARHGEF2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Binds to CFLAR/CLARP and CASP1 via their CARD domains. Binds to BIRC3/c-IAP1 and BIRC2/c-IAP2, TRAF1, TRAF2, TRAF5 and TRAF6. May be a component of both the TNFRSF1A and TNRFSF5/CD40 receptor complex. Interacts with NOD1. Interacts (via CARD domain) with NOD2 (via CARD domain). Interacts with MAP3K4; this interaction sequesters RIPK2 from the NOD2 signaling pathway. Interacts with IKBKG/NEMO. The polyubiquitinated protein interacts with MAP3K7/TAK1. Interacts with XIAP/BIRC4. Interacts with NLRP10. Interacts with CARD9.

Alternative Nomenclature

CARD carrying kinase antibody

CARD CONTAINING ICE ASSOCIATED KINASE antibody

CARD containing interleukin 1 beta converting enzyme associated kinase antibody

CARDIAK antibody

CLARP kinase antibody

GIG 30 antibody

Growth inhibiting gene 30 antibody

Receptor interacting protein 2 antibody

Receptor interacting serine threonine kinase 2

RICK antibody

RIP-like-interacting CLARP kinase antibody

General Information

Product

TICAM2 Antibody

Description

Affinity Purified TRIF-related adapter molecule Antibody

Verified Applications

DB, ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on TRIF-related adapter molecule.

Accession #

GenBank: AAO74498.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q86XR7

Overview

Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4. May also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB; however, reports are controversial. Involved in IL-18 signaling and is proposed to function as a sorting adaptor for MYD88 in IL-18 signaling during adaptive immune response.

Molecular Function

phospholipid binding, signal transducer activity

Subcellular Location

Isoform 1 : Cytoplasm. Golgi apparatus. Cell membrane. Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum.

Expression

Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).

Structure

Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs i

Alternative Nomenclature

Putative NF-kappa-B-activating protein 502 antibody

TICAM2 antibody

TIR containing adapter molecule 2 antibody

TIR domain-containing adapter molecule 2 antibody

TIRAP3 antibody

TIRP antibody

Toll-like receptor adaptor protein 3 antibody

Toll/interleukin-1 receptor domain-containing protein antibody

TRAM antibody

TRIF-related adapter molecule antibody

General Information

Product

MAPK14 Antibody

Description

Affinity Purified p38 Mitogen Activated Protein Kinase 14 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human Mitogen Activated Protein Kinase 14 protein.

Accession #

GenBank: ACI00233.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q16539

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation.

Molecular Function

ATP binding; MAP kinase activity; MAP kinase kinase activity; NFAT protein binding; Protein serine/threonine kinase activity

Subcellular Location

Cytoplasm; Nucleus

Structure

Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR (By similarity). This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation (By similarity). Interacts with SPAG9 and GADD45A (By similarity). Interacts with CDC25B, CDC25C, DUSP1, DUSP10, DUSP16, NP60, SUPT20H and TAB1. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B.

Alternative Nomenclature

CSAID-binding protein antibody

CSBP antibody

Cytokine suppressive anti-inflammatory drug-binding protein antibody

EXIP antibody

MAP kinase 14 antibody

MAP kinase p38 alpha antibody

MAPK14 antibody

MAX-interacting protein 2 antibody

Mitogen-activated protein kinase 14 antibody

p38 antibody

p38 MAP kinase antibody

SAPK2A antibody

Stress-activated protein kinase 2a antibody