General Information

Product

PREX2 Antibody Affinity Purified

Description

Affinity Purified PtdIns(3,4,5)-dependent Rac exchanger 2a Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 720-900 on PREX2 protein.

Accession #

GenBank:  DAA05334.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q70Z35

Overview

Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase.

Molecular Function

Rac GTPase activator activity, Rac guanyl-nucleotide exchange factor activity

Expression

Isoform 1 is highly expressed in skeletal muscle, heart and placenta, absent from peripheral blood leukocytes. Isoform 2 is expressed in skeletal muscle, kidney, small intestine, and placenta. Isoform 3 is expressed in the heart.

Alternative Nomenclature

4 antibody

5)-dependent Rac exchanger 2 antibody

5-trisphosphate-dependent Rac exchanger 2 protein antibody

DEP domain-containing protein 2 antibody

DEP.2 antibody

Phosphatidylinositol 3 antibody

PtdIns(3,4,5)-dependent Rac exchanger 2 antibody

General Information

Product

Dexras1 Antibody

Description

Affinity Purified Dexamethasone-induced Ras-related protein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Panda

Immunogen

Synthetic peptide:

HSDLMYIREKTSVGSQAKDKERC

corresponding to amino acids 256-278 of Mouse Dexras 1 protein.

Accession #

NCBI: NP_033052.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O35626

Overview

Small GTPase, negatively regulates the transcription regulation activity of the APBB1/FEF65-APP complex via its interaction with APBB1/FE65.

Molecular Function

GTP binding

Subcellular Location

Cell membrane, Lipid-anchor, Cytoplasmic side

Expression

Expressed in brain, heart, kidney and liver

Structure

Component of a complex, at least composed of APBB1, RASD1/DEXRAS1 and APP.  Interacts with APBB1/FE65.  Forms a ternary complex with CAPON and NOS1.

Alternative Nomenclature

Activator of G protein signaling 1 antibody

AGS1 antibody

Dexamethasone-induced Ras-related protein 1 antibody

Dexras 1 antibody

RAS dexamethasone induced 1 antibody

Ras related protein antibody

RASD 1 antibody

General Information

Product

RASGRF2 Antibody

Description

Affinity Purified Ras-specific guanine nucleotide-releasing factor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 1100-1200 on human RASGRF2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O14827

Overview

Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP.  Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation.

Molecular Function

Guanine-nucleotide releasing factor, Rho guayl-nucleotide exchange factor activity

Subcellular Location

Cytoplasm, Cell membrane; Peripheral membrane protein, Endoplasmic reticulum membrane; Peripheral membrane protein

Expression

Widely expressed with higher expression in the brain, followed by heart, lung, pancreas and kidney.  Detected in placenta.  Expressed in brain and lung.

Structure

Homooligomer and heterooligomer with RASGRF1.  Interacts with Ras and RAC1.  Interacts in a calcium-dependent manner with calmodulin.

Alternative Nomenclature

GRF2 antibody

Ras guanine nucleotide exchange factor 2 antibody

Ras-GRF2 antibody

General Information

Product

RIC8A Antibody Affinity Purified

Description

Affinity Purified Synembryn-A isoform 1 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acids 500-535 on RIC8A protein

Accession #

NCBI:  NP_068751.4

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

Q9NPQ8

Overview

Guanine nucleotide exchange factor (GEF) which can activate some G-alpha proteins.  Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP.

Molecular Function

guanyl-nucleotide exchange factor activity, Guanine-nucleotide releasing factor

Subcellular Location

Interacts with GDP-bound G alpha proteins GNAI1, GNAO1 and GNAQ, and with GNA13 with lower affinity.  Does not interact with G-alpha proteins when they are in complex with subunits beta and gamma.

Alternative Nomenclature

Protein Ric-8A antibody

resistance to inhibitors of cholinesterase 8 homolog A (C. elegans) antibody

RIC8 antibody

Synembryn-A antibody

General Information

Product

RICH2 Antibody

Description

Affinity Purified Rho GTPase-activating protein 44 isoform 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to N terminal amino acids 1-60 of Mouse RICH2.

Sequence:

MKKQFNRMRQ LANQTVGRA E KTEVLSED LL QVEKRLE LVK QVSHST HKKL TACLQ GQQGA

Accession #

NCBI:  NP_778168.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:250-1:500

Protein

Uniprot #

Q5SSM3

Overview

GTPase-activating protein (GAP) that stimulates the GTPase activity of the Rho-type GTPases.  Thereby controlling the Rho-type GTPases cycling between their active GTP-bound and inactive GDP bound states.

Molecular Function

GTPase activator activity, phospholipid binding, GTPase activation

Subcellular Location

Cell projection, Recycling endosome, Cell junction

Expression

Expressed in hippocampal neurons at protein level

Structure

Interacts with BST2, SHANK3.

Alternative Nomenclature

KIAA0672 antibody

NPC A 10 antibody

Rho GTPase activating protein RICH2 antibody

RhoGAP interacting with CIP4 homologs protein 2 antibody

General Information

Product

EDG8 Antibody

Description

Affinity Purified Sphingosine 1-phosphate receptor 5 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 380-400 of rat S1P5R/EDG8 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9JKM5

Overview

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/O)alpha and G(12) subclass of heteromeric G-proteins.

Molecular Function

G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Found almost exclusively in brain and skin. Ubiquitously detected in different brain regions with very prominent expression in lower brain regions such as the midbrain, pons, medulla, and spinal cord. Expressed predominantly within white matter tracts of

Alternative Nomenclature

EDG 8 antibody

Endothelial differentiation G protein coupled receptor 8 antibody

Endothelial differentiation sphingolipid G protein coupled receptor 8 antibody

S1P receptor 5 antibody

S1P receptor Edg 8 antibody

Sphingosine 1 Phosphate Receptor 5 antibody

Sphingosine 1-phosphate receptor Edg-8 antibody

SPPR2 antibody

General Information

Product

SPHK1 Antibody

Description

Affinity Purified Sphingosine kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human SPHK1 protein.

Accession #

GenBank: CAM17117.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8CI15

Overview

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Molecular Function

ATP binding, calmodulin binding, DNA binding, magnesium ion binding, NAD+kinase activity, sphinganine kinase activity

Subcellular Location

Cytoplasm, nucleus, cell membrane

Alternative Nomenclature

SK1 antibody

Sphingosine Kinase 1 antibody

SPHK 1 antibody

SPHK antibody

SPK antibody

SPK 1 antibody

General Information

Product

Chimaerin beta 3 Antibody

Description

Affinity Purified Beta-Chimaerin isoform 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-100 on human beta-chimaerin isoform 3 protein.

Accession #

NCBI:  NP_001279998.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.71 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P52757

Overview

GTPase-activating protein for p21-rac.  May play role in progression from low-grade to high-grade tumors.

Molecular Function

Metal binding; Zinc

Subcellular Location

Membrane

Expression

Brain, pancreas, heart, placenta.  Weakly expressed in kidney and liver.

Structure

Contains 1 phorbol-ester/DAG-type zinc finger; 1 Rho-GAP domain; 1 SH2 domain.

Alternative Nomenclature

ARHGAP3 antibody

BCH antibody

Beta chimerin antibody

Chimaerin3 antibody

CHN3 antibody

MGC138360 antibody

Rho GTPase activating protein 3 antibody

RHOGAP3 antibody

General Information

Product

ARF1 Antibody

Description

Affinity Purified ADP-ribosylation factor 1 Antibody

Verified Applications

ELISA, ICC/IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500. Predicted Molecular Weight 21 kDa

Protein

Uniprot #

P84079

Overview

GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.

Molecular Function

ADP-ribosyltransferase; GTP binding; ER-Golgi transport

Subcellular Location

Gogli apparatus

Expression

Cell junction; Membrane; Synapse

Structure

Belongs to the small GTPase superfamily, Arf family

Alternative Nomenclature

DJ133P16.1 antibody

General Information

Product

ARF4 Antibody

Description

Affinity Purified ADP-ribosylation factor 4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on ARF4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.71-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P61751

Overview

GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.

Molecular Function

ADP-ribosyltransferase; GTP binding; ER-Golgi transport

Subcellular Location

Gogli apparatus

Expression

Cell junction; Membrane; Synapse

Structure

Belongs to the small GTPase superfamily, Arf family

Alternative Nomenclature

ADP ribosylation factor 2 antibody

ADP ribosylation factor 4 antibody