General Information

Product

RIOK1 Antibody

Description

Affinity Purified Serine/threonine-protein kinase RIO1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Serine/threonine-protein kinase RIO1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9BRS2

Molecular Function

ATP binding

hydrolase activity

metal ion binding

protein serine/threonine kinase activity

Subcellular Location

Cyotosol

Structure

Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.

Alternative Nomenclature

AD034

bA288G3.1

RIO kinase 1

RIOK1

Serine/threonine-protein kinase rio1

General Information

Product

WEE2 Antibody

Description

Affinity Purified Wee1-like protein kinase isoform 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 60-110 on mouse Wee1-like protein kinase isoform 2.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polylclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q66JT0

Molecular Function

ATP binding

magnesium ion binding

non-membrane spanning protein tyrosine kinase activity

protein tyrosine kinase activity

Subcellular Location

Nucleus, Cytoplasm

Expression

Ovary-specific.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.

Alternative Nomenclature

WEE1 homolog 2 (S. pombe) antibody

Wee1-like protein kinase 1B antibody

Wee1-like protein kinase 2 antibody

WEE1B antibody

Wee1B kinase antibody

Wee2 antibody

WEE2_HUMAN antibody

General Information

Product

IRAK1 Antibody

Description

Affinity Purified Interleukin-1 receptor-associated kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid reigon 700-750 on mouse Interleukin-1 receptor-associated kinase 1 protein.

Accession #

NCBI:  NP_001171444

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polylclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

B1AUW6

Molecular Function

ATP binding

heat shock protein binding

kinase activity

NF-kappaB-inducing kinase activity

protein heterodimerization activity

protein homodimerization activity

protein kinase activity

protein serine/threonine kinase activity

Subcellular Location

Nucleus, Cytoplasm, Lipid droplet

Expression

Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Structure

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Alternative Nomenclature

Il1rak antibody

Interleukin 1 receptor associated kinase 1 antibody

IRAK antibody

Irak1 antibody

mPLK antibody

Pelle antibody

Pelle homolog antibody

Plpk antibody

General Information

Product

IRAKM Antibody

Description

Affinity Purified Interleukin-1 receptor-associated kinase 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on mouse Interleukin-1 receptor-associated kinase 3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polylclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9Y616

Overview

Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.

Molecular Function

ATP binding

magnesium ion binding

protein heterodimerization activity

protein homodimerization activity

protein serine/threonine kinase activity

Subcellular Location

Nucleus, Cytoplasm

Expression

Expressed predominantly in peripheral blood lymphocytes.

Structure

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Alternative Nomenclature

ASRT5 antibody

IL 1 receptor associated kinase M antibody

Interleukin 1 receptor associated kinase 3 antibody

Interleukin 1 receptor associated kinase M antibody

IRAK M antibody

IRAK-3 antibody

IRAK-M antibody

Irak3 antibody

General Information

Product

P70 S6 Kinase beta Antibody

Description

Affinity Purified Ribosomal protein S6 kinase beta Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human Ribosomal protein S6 kinase beta protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P23443

Molecular Function

ATP binding

kinase activity

protein kinase activity

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

ribosomal protein S6 kinase activity

Subcellular Location

Cell junction, Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus, Synapse, Synaptosome

Expression

Widely expressed

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Alternative Nomenclature

70 kDa ribosomal protein S6 kinase 2 antibody
KS6B2_HUMAN antibody
p70 beta antibody
p70 ribosomal S6 kinase beta antibody
p70 S6 kinase beta antibody
p70 S6K-beta antibody
p70 S6KB antibody
p70-beta antibody
p70-S6K 2 antibody
Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
Ribosomal protein S6 kinase B2 antibody
Rps6kb2 antibody
S6 kinase related kinase antibody
S6K beta 2 antibody
S6K beta antibody
S6K2 antibody
Serine/threonine protein kinase 14 beta antibody
STK14B antibody

General Information

Product

PP2A-alpha Antibody

Description

Affinity Purified Serine / Threonine-Protein Phosphatase 2A , B56- Alpha subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 410-486 on human Serine / Threonine-Protein Phosphatase 2A , B56- Alpha subunit.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q15172

Overview

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

Molecular Function

kinase binding

phosphoprotein phosphatase activity

protein phosphatase activator activity

protein phosphatase regulator activity

Subcellular Location

Centromere, Chromosome, Cytoplasm, Nucleus

Expression

Widely expressed with the highest expression in heart and skeletal muscle.

Structure

Belongs to the phosphatase 2A regulatory subunit B56 family.

Alternative Nomenclature

PP2A alpha antibody
PP2AA_HUMAN antibody
PP2Aalpha antibody
PP2Ac antibody
PP2CA antibody
PP2Calpha antibody
PPP2CA antibody
Protein phosphatase 2 (formerly 2A) catalytic subunit alpha isoform antibody
Protein phosphatase 2 catalytic subunit alpha isoform antibody
Protein phosphatase 2, catalytic subunit, alpha isozyme antibody
Protein phosphatase 2A catalytic subunit, alpha isoform antibody
Replication protein C antibody
RP C antibody
RP-C antibody
RPC antibody
Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

General Information

Product

MLKL Antibody

Description

Affinity Purified Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 400-450 on mouse Mixed lineage kinase domain-like protein MLKL.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9D2Y4

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414). Within this complex, may play a role in the proper targeti

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody

General Information

Product

Phospho-MLKL Antibody

Description

Affinity Purified Phosphorylated Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 350-400 on human Mixed lineage kinase domain-like protein MLKL

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q8NB16

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414).

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody