MAP3K8 Antibody

General Information

Product

MAP3K8 Antibody

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 8 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 360-467 on human MAP3K8 protein.

Accession #

GenBank: AAI13567.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P41279

Overview

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Expressed in several normal tissues and human tumor-derived cell lines.

Structure

Forms a ternary complex with NFKB1/p105 and TNIP2. Interacts with NFKB1; the interaction increases the stability of MAP3K8 but inhibits its MEK phosphorylation activity, whereas loss of interaction following LPS stimulation leads to its degradation. Interacts with CD40 and TRAF6; the interaction is required for ERK activation. Interacts with KRS2; the interaction inhibits ERK and NF-kappa-B activation.

Alternative Nomenclature

c COT antibody

Cancer Osaka thyroid oncogene antibody

COT antibody

COT proto oncogene serine/threonine protein kinase antibody

EST antibody

ESTF antibody

Ewing sarcoma transformant antibody

FLJ10486 antibody

MEKK8 antibody

Mitogen activated protein kinase kinase kinase 8 antibody

Proto oncogene cCot antibody

Serine/threonine-protein kinase cot antibody

TPL 2 antibody

Tumor progression locus 2 antibody