General Information

Product

RUNX1 / AML1 Antibody Affinity Purified

Description

Affinity Purified RUNX1 Protein Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 1-80 on human RUNX1 protein.

Accession #

GenBank: AAI36381.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.53 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q01196

Overview

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis.

Molecular Function

ATP binding, DNA binding, calcium ion binding

Subcellular Location

Nucleus

Expression

Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood.

Structure

Heterodimer with CBFB. RUNX1 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Isoform AML-1L can neither bind DNA nor heterodimerize.

Alternative Nomenclature

Acute myeloid leukemia 1 protein antibody

alpha subunit core binding factor antibody

AML1 EVI 1 fusion protein antibody

Aml1 oncogene antibody

AMLCR 1 antibody

CBF alpha 2 antibody

Core binding factor runt domain alpha subunit 2 antibody

Core-binding factor subunit alpha-2 antibody

EVI1 antibody

HGNC antibody

Oncogene AML 1 antibody

PEBP2 alpha B antibody

Polyomavirus enhancer binding protein 2 alpha B subunit antibody

Run1 antibody

Runt-related transcription factor 1 antibody

SL3/AKV core binding factor alpha B subunit antibody

General Information

Product

SIRT7 Antibody

Description

Affinity Purified Sirtuin (silent mating type information regulation 2 homolog) 7 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 340-390 on human SIRT7 protein.

Accession #

GenBank: AAH17305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NRC8

Overview

NAD-dependent protein deacetylase that specifially mediates deacetylation of histone H3 at 'Lys-18'. Displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression.

Molecular Function

Chromatin binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K18 specific), metal ion binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: Located close to the nuclear membrane when in cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

Expression

Detected in liver, spleen and testis. Detected in embryos.

Structure

Belongs to the Sirtuin Family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 7 antibody

Regulatory protein SIR2 homolog 7 antibody

Silent mating type information regulation 2 homolog antibody

SIR2 L7 antibody

SIR2 like protein 7 antibody

Sir2 related protein type 7 antibody

SIRT7 antibody

Sirtuin 7 antibody

Sirtuin type 7 antibody

General Information

Product

SIRT5 Antibody

Description

Affinity Purified Sirtuin 5 (silent mating type information regulation 2 homolog) 5 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 260-310 on rat Sirtuin 5 protein.

Accession #

NCBI: NP_001004256.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q68FX9

Overview

NAD-dependent lysine demalonylase and desuccinylase that specifically removes malonyl and succinyl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro.

Molecular Function

Hydrolase; Metal-binding; NAD; Zinc

Subcellular Location

Isoform 1:  Cytoplasm, Mitochondrion  Isoform 2:  Mitochondrion

Expression

Widely expressed.

Structure

Interacts with CPS1 (By similarity). Monomer. Homodimer. Forms homodimers upon suramin binding.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 5 antibody

Regulatory protein SIR2 homolog 5 antibody

Silent mating type information regulation 2 Sir2 like 5 antibody

SIR2-like protein 5 antibody

SIR2L5 antibody

Sirtuin type 5 antibody

General Information

Product

ASF1A Antibody

Description

Affinity Purified Histone Chaperone ASF1A Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 60-120 on human AF1A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9Y294

Overview

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Molecular Function

Transcription regulation

Subcellular Location

Nucleus

Structure

Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with CABIN1, HAT1, HIRA, NASP, TAF1, TLK1, TLK2 and UBN1. Interacts with CDAN1. Found in cytosolic coplex with CDAN1, ASF1B, IPO4 and histones H3.1 and H4. Interacts with CREBBP.

Alternative Nomenclature

Anti silencing function 1A antibody

asf1a antibody

CCG1-interacting factor A antibody

CGI 98 antibody

CIA antibody

hAsf1 antibody

hAsf1a antibody

hCIA antibody

Histone chaperone ASF1A antibody

HSPC146 antibody

Hypothetical protein FLJ22085 antibody

General Information

Product

JMJD2D Antibody Affinity Purified

Description

Affinity Purified Jumonji domain-containing protein 2D Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 340-390 on human Jumonji domain containg 2D protein.

Accession #

GenBank: AAI19011.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q6B0I6

Overview

Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.

Molecular Function

Chromatin regulator, Dioxygenase, Oxidoreductase

Subcellular Location

Nucleus

Structure

Belongs to the JHDM3 histone demethylase family.

Contains 1 JmjC domain.

Contains 1 JmjN domain.

Alternative Nomenclature

JHD3D antibody

JHDM3D antibody

JmjC domain-containing histone demethylation protein 3D antibody

Jumonji domain-containing protein 2D antibody

Kdm4d antibody

Lysine-specific demethylase 4D antibody

General Information

Product

C15orf42 Antibody Affinity Purified

Description

Affinity Purified Treslin Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human Treslin protein.

Accession #

NCBI:  NP_689472.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q7Z2Z1

Overview

Regulates triggering of DNA replication onto replication origins, required for transition from pre-replication complex to pre-initiation complex. Required to prevent mitotic entry after treatment with ionizing radiation.

Molecular Function

Chromatin binding

Subcellular Location

Nucleus

Alternative Nomenclature

C15orf42 antibody

chromosome 15 open reading frame 42 antibody

replication-stimulating protein antibody

SLD3 antibody

SLD3 homolog antibody

SLD3, yeast , homolog of antibody

TICRR antibody

TopBP1-interacting checkpoint and replication regulator antibody

Treslin antibody

General Information

Product

SIRT3 Antibody

Description

Affinity Purified NAD-dependent protein deacetylase sirtuin-3, mitochondrial Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide:

RDLVGPFVLSPRRKDVVQL

corresponding to amino acids 203-221 of rat SIRT3 protein.

Accession #

NCBI:  NP_001099783

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B2RZ31

Molecular Function

enzyme binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K14 specific)

Alternative Nomenclature

Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody

NAD dependent deacetylase sirtuin 3 mitochondrial antibody

Regulatory protein SIR2 homolog 3 antibody

Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody

SIR 2 like protein 3 antibody

SIR 3 antibody

SIR2 L3 antibody

Sirtuin 3 antibody

Sirtuin type 3 antibody

General Information

Product

SIRT7 Antibody

Description

Affinity Purified Sirtuin (silent mating type information regulation 2 homolog) 7 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on mouse SIRT7 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8BKJ9

Overview

NAD-dependent protein deacetylase that specifially mediates deacetylation of histone H3 at 'Lys-18'. Displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression.

Molecular Function

Chromatin binding, NAD+ binding, NAD-dependent histone deacetylase activity (H3-K18 specific), metal ion binding

Subcellular Location

Cytoplasm, nucleus, nucleolus. (Note: Located close to the nuclear membrane when in cytoplasm. Associated with chromatin. Associated with rDNA promoter and transcribed region. Associated with nucleolar organizer regions during mitosis.

Expression

Detected in liver, spleen and testis. Detected in embryos.

Structure

Belongs to the Sirtuin Family. Class IV subfamily. Contains 1 deacetylase sirtuin-type domain.

Alternative Nomenclature

NAD dependent deacetylase sirtuin 7 antibody

Regulatory protein SIR2 homolog 7 antibody

Silent mating type information regulation 2 homolog antibody

SIR2 L7 antibody

SIR2 like protein 7 antibody

Sir2 related protein type 7 antibody

SIRT7 antibody

Sirtuin 7 antibody

Sirtuin type 7 antibody

General Information

Product

LARP7 Antibody Affinity Purified

Description

Affinity Purified LARP7 protein Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 360-410 on human LARP7 protein.

Accession #

GenBank:   AAI07710.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q4G0J3

Overview

Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.

Molecular Function

RNA-binding; RNA processing

Subcellular Location

Nucleus

Structure

Integral part of the 7SK RNP complex. Specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA. On stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex.

Alternative Nomenclature

HDCMA18P antibody

La related protein 7 antibody

La ribonucleoprotein domain family member 7 antibody

P TEFb interaction protein for 7SK stability antibody

PIP7S antibody

General Information

Product

LARP7 Antibody Affinity Purified

Description

Affinity Purified LARP7 protein Antibody N-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human LARP7 protein.

Accession #

GenBank:   AAI07710.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q4G0J3

Overview

Negative transcriptional regulator of polymerase II genes, acting by means of the 7SK RNP system. Within the 7SK RNP complex, the positive transcription elongation factor b (P-TEFb) is sequestered in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.

Molecular Function

RNA-binding; RNA processing

Subcellular Location

Nucleus

Structure

Integral part of the 7SK RNP complex. Specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA. On stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex.

Alternative Nomenclature

HDCMA18P antibody

La related protein 7 antibody

La ribonucleoprotein domain family member 7 antibody

P TEFb interaction protein for 7SK stability antibody

PIP7S antibody