General Information

Product

Eprhin Receptor A4 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-A receptor 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 550-650 on human EphA R4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q03137

Overview

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highest expression in the adult brain and retina and also detectable in kidney, lung, skeletal muscle and thymus. Not detected in heart and liver.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts (via

Alternative Nomenclature

Cek 8 antibody

EK8 antibody

eph receptor a4 antibody

EPH-like kinase 8 antibody

EphA4 antibody

Ephrin receptor Eph A4 antibody

hEK8 antibody

Receptor protein tyrosine kinase HEK 8 antibody

Sek 1 antibody

SEK antibody

TYRO 1 protein tyrosine kinase antibody

Tyrosine-protein kinase receptor SEK antibody

General Information

Product

Ephrin Receptor A7 Antibody

Description

Affinity Purified EPHA7 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding taken within amino acid region 500-550 on human Ephrin type-A receptor 7 protein.

Accession #

NCBI:  NP_004431

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q15375

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. 

Molecular Function

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Single-pass type 1 membrane protein

Expression

Widely Expressed

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (via PDZ-binding motif) with GRIP1 and PICK.

Alternative Nomenclature

Cek 11 

Developmental kinase 1 

EBK 

EHK3 

EK11 

Embryonic brain kinase 

Eph homology kinase 3 

Eph receptor A7 

EPH-like kinase 11 

EphA7 

Ephrin type-A receptor 7 

hEK11 

MDK 1 

Receptor protein tyrosine kinase HEK 11 

Tyrosine protein kinase receptor EHK 3 

General Information

Product

Eprhin Receptor A6 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-A receptor 6 isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 800-900 on human EphA R6 protein.

Accession #

NCBI:  NP_001073917

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B3KS12

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity).

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Expressed in brain and testis.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

Alternative Nomenclature

Ehk 2 antibody

EPH homology kinase 2 antibody

Eph Receptor A6 antibody

ephrin receptor EphA6 antibody

Hek12 antibody

General Information

Product

Eprhin Receptor A5 Antibody Affinity Purified

Description

Affinity Purified Ephrin Type-1 Receptor isoform a Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 600-700 on human EphA R5 protein.

Accession #

NCBI:  NP_004430

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P54756

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

Alternative Nomenclature

Brain-specific kinase antibody

bsk antibody

CEK 7 antibody

Eck like sequence 1 antibody

EHK 1 antibody

EK7 antibody

Els 1 antibody

Eph homology kinase 1 antibody

EPH-like kinase 7 antibody

EphA5 antibody

Ephrin type-A receptor 5 antibody

HEK 7 antibody

Rek 7 antibody

TYRO 4 antibody

Tyrosine protein kinase receptor EHK 1 antibody

General Information

Product

Eprhin Receptor B2 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-B receptor 2 isoform 2 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-150 on mouse Ephrin BR2 protein.

Accession #

NCBI:  NP_034272

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

A3KG01

Overview

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane

Structure

Contains sterile alpha motif domain; Conains protein kinase domain

Alternative Nomenclature

Cek 6 antibody

EK6 antibody

ELK antibody

Elkh antibody

Eph tyrosine kinase 2 antibody

EPH-like kinase 6 antibody

EPH2 antibody

EphB1 antibody

Ephrin receptor Eph B1 precursor antibody

Ephrin type B receptor 1 antibody

EPHT2 antibody

HEK 6 antibody

NET antibody

Neuronally expressed EPH related tyrosine kinase antibody

Tyrosine-protein kinase receptor EPH-2 antibody

General Information

Product

Dopamine D5 Receptor Antibody Affinity Purified

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken  within amino acid region 428-478 on mouse Dopamine Receptor D5 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:50,000

ELISA

1:50,000

Western Blot

1:1,000-1:1,5000

Protein

Uniprot #

Q8BLD9

Overview

Dopamine receptor mediated by G proteins which inhibit adenylyl cyclase.  Modulates circadian rhtym of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells.

Molecular Function

Dopamine binding; Biological rhythms

Subcellular Location

Cell membrane; Multi-pass membrane

Structure

Belongs to G-protein coupled receptor 1 family; Transmembrane helix

Alternative Nomenclature

D(1B) dopamine receptor antibody

D1B dopamine receptor antibody

DBDR antibody

DRD 1B antibody

DRD 5 antibody

DRD1L2 antibody

General Information

Product

Dopamine D5 Receptor Antibody Affinity Purified

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Rat

Immunogen

Synthetic peptide corresponding to unique sequence on Dopamine Receptor D5 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

DB

1:50,000

ELISA

1:50,000

Western Blot

1:1,000-1:1,5000

Protein

Uniprot #

Q8BLD9

Overview

Dopamine receptor mediated by G proteins which inhibit adenylyl cyclase.  Modulates circadian rhtym of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells.

Molecular Function

Dopamine binding; Biological rhythms

Subcellular Location

Cell membrane; Multi-pass membrane

Structure

Belongs to G-protein coupled receptor 1 family; Transmembrane helix

Alternative Nomenclature

D(1B) dopamine receptor antibody

D1B dopamine receptor antibody

DBDR antibody

DRD 1B antibody

DRD 5 antibody

DRD1L2 antibody

General Information

Product

GAD65 Antibody

Description

Affinity Purified Glutamate Decarboxylase 65 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-110 on human Glutamate decarboxylase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q05329

Overview

Its function is to catalyze the production of GABA.

Molecular Function

Decarboxylase, Lyase, glutamate binding

Subcellular Location

Cytoplasm, Cell junction, Lipid-anchor, Golgi apparatus membrane

Structure

Homodimer

Alternative Nomenclature

65 kDa glutamic acid decarboxylase antibody

DCE 2 antibody

GAD 2 antibody

GAD 65 antibody

Glutamate decarboxylase 2 (pancreas) antibody

Glutamate Decarboxylase 65 antibody

MGC161605 antibody

MGC161607 antibody

General Information

Product

Glucose Transporter GLUT3 Antibody

Description

Affinity Purified Solute carrier family 2, facilitated glucose transporter member 3 Antibody

Verified Applications

CM, ELISA, Flow cytometry, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-493 on mouse SLC2A3 protein.

Accession #

NCBI: NP_008862

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confotcal Microscopy

1:250

DOT Blot

1:25,000

ELISA

1:25,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:8,000

Protein

Uniprot #

Q4FJP8

Overview

Facilitative glucose transporter.

Molecular Function

Sugar Transport

Subcellular Location

Membrane; Multi-pass membrane protein

Expression

Brain and many other tissues.

Structure

Transmembrane helix

Alternative Nomenclature

brain antibody

FLJ90380 antibody

Glucose transporter type 3 brain antibody

GLUT-3 antibody

SLC2A3 antibody

Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3 antibody

General Information

Product

GABA B Receptor 2 Antibody

Description

Affinity Purified Gamma-aminobutyric acid receptor subunit beta-2 Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Cat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human gamma-aminobutyric acid receptor subunit beta-2 protein.

Accession #

NCBI:  NP_068711.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P47870

Overview

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.

Molecular Function

Chloride channel activity; GABA-A receptor activity; Inhibitory extracellular ligand-gated ion channel activity

Subcellular Location

Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein

Expression

Isoform 1 and isoform 2 show reduced expression in schizophrenic brain. Isoform 3 shows increased expression in schizophrenic and bipolar disorder brains while isoform 4 shows reduced expression.

Structure

Binds UBQLN1. Interacts with KCT8, KCTD12 and KCTD16; this interaction determines the pharacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents. Heteropentamer, formed by a combination of alpha, beta, gamma, delta and rho chains.

Alternative Nomenclature

GABA-B-R2 antibody

Gamma aminobutyric acid (GABA) B receptor 2 antibody

Gb1 antibody

GPRC3A antibody

Seven transmembrane helix receptor antibody