|
General Information
|
|
|
Product
|
HMGB1 Antibody
|
|
Description
|
Affinity Purified High mobility group protein B1 Antibody
|
|
Verified Applications
|
ELISA, IHC, IP, WB
|
|
Host
|
Rabbit
|
|
Species Cross Reactivity
|
Human, Mouse, Rat
|
|
Immunogen
|
Synthetic peptide taken within amino acid region 150-200 on human High mobility group protein B1 protein.
|
|
Physical Properties
|
|
|
Quantity
|
100 µg
|
|
Volume
|
200 µl
|
|
Concentration
|
0.5-1.5 µg/µl in antibody stabilization buffer
|
|
Immunoglobulin
|
IgG
|
|
Form
|
Affinity Purified
|
|
Purification Method
|
Immobilized antigen affinity chromatography
|
|
Clonality
|
Polyclonal
|
|
Storage
|
-20⁰C for long term storage
|
|
Application Protocol
|
|
ELISA
|
1:50,000
|
| Immunohistochemistry |
1:50-1:150 |
|
Immunoprecipitation
|
1:200
|
|
Western Blot
|
1:2,500-1:5,000
|
|
Protein
|
|
Uniprot #
|
P09429
|
|
Overview
|
Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids.
|
|
Molecular Function
|
bubble DNA binding
chemoattractant activity
C-X-C chemokine binding
cytokine activity
damaged DNA binding
DNA binding, bending
DNA binding transcription factor activity
DNA polymerase binding
double-stranded DNA binding
four-way junction DNA binding
integrin binding
lipopolysaccharide binding
lyase activity
phosphatidylserine binding
RAGE receptor binding
repressing transcription factor binding
RNA binding
single-stranded DNA binding
supercoiled DNA binding
transcription factor binding
|
|
Subcellular Location
|
Extracellular region or secreted, nucleus, endosome, plasma membrane, chromosome, cytoplasm, endoplasmic reticulum-golgi intermediate compartment
|
|
Expression
|
Ubiquituous. Expressed in platelets.
|
|
Structure
|
Belongs to the HMGB family.
|
|
Alternative Nomenclature
|
Amphoterin antibody
Chromosomal protein, nonhistone, HMG1 antibody
High mobility group box 1 antibody
High mobility group protein 1 antibody
High mobility group protein B1 antibody
high-mobility group (nonhistone chromosomal) protein 1 antibody
HMG-1 antibody
HMG3 antibody
HMGB 1 antibody
NONHISTONE CHROMOSOMAL PROTEIN HMG1 antibody
SBP 1 antibody
Sulfoglucuronyl carbohydrate binding protein antibody
|
| |
|
HMGB1 Antibody BIOTIN
HMGB1 Antibody FITC
HMGB1 Blocking Peptide
HMGB1 Positive Control
