General Information

Product

Occludin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-523 on rat Occludin protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q6P6T5

Overview

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.

Molecular Function

protein domain specific binding

Subcellular Location

Cell membrane

Structure

he C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction.

Alternative Nomenclature

BLCPMG antibody

Occludin antibody

Ocln antibody

OCLN_HUMAN antibody

Phosphatase 1 regulatory subunit 115 antibody

PPP1R115 antibody

PTORCH1 antibody

Tight junction protein occludin antibody

General Information

Product

CD146 Antibody

Description

Affinity Purified Cell Surface Glycoprotein MUC18 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Rat

Immunogen

Synthetic peptide taken within amino acid region 330-353 on rat Cell Surface Glycoprotein MUC18 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9EPF2

Overview

lays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Detected in lung, uterus and placenta (at protein level). Detected in heart, lung, kidney, adrenal gland, intestine, testis, skeletal muscle and aorta. Detected at low levels in adult brain, in particular in brain stem and spinal cord, but also in hippocampus, olfactory bulb and striatum (at protein level).

Structure

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

A32 antigen antibody

CD 146 antibody

Cell surface glycoprotein MUC18 antibody

Cell surface glycoprotein P1H12 antibody

Gicerin antibody

Mcam antibody

Melanoma adhesion molecule antibody

Melanoma associated antigen A32 antibody

Melanoma associated antigen MUC18 antibody

Melanoma associated glycoprotein MUC18 antibody

Melanoma cell adhesion molecule antibody

Melanoma-associated antigen A32 antibody

Melanoma-associated antigen MUC18 antibody

MelCAM antibody

MUC 18 antibody

S endo 1 antibody

S endo 1 endothelial associated antigen antibody

General Information

Product

VGluT1 Antibody

Description

Affinity Purified Vesicular Glutamate transporter 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on mouse Vesicular Glutamate transporter 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q3TXX4

Overview

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.

Molecular Function

extracellularly glutamate-gated chloride channel activity

inorganic phosphate transmembrane transporter activity

sodium:inorganic phosphate symporter activity

sodium-dependent phosphate transmembrane transporter activity

Subcellular Location

synaptic vesicle membrane

Membrane  Curated; Multi-pass membrane protein 

synaptosome

Expression

Expressed in hippocampus (at protein level). Expressed in the molecular layer of the cerebellum and in retina.

Structure

Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.

Alternative Nomenclature

BNPI antibody

Brain specific Na (+) dependent inorganic phosphate cotransporter antibody

Slc17a7 antibody

Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody

Solute carrier family 17 member 7 antibody

Vesicular glutamate transporter 1 antibody

VGluT1 antibody

General Information

Product

PARD6B Antibody

Description

Affinity Purified Partitioning Defective 6 homolog beta Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 300-350 on human Partitioning Defective 6 homolog beta.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9BYG5

Overview

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Molecular Function

protein kinase C binding

Rho GTPase binding

Subcellular Location

Cell membrane, Cytoplasm

Expression

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Structure

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Alternative Nomenclature

OTTHUMP00000031269 antibody

PAR 6 beta antibody

Par 6 partitioning defective 6 homolog beta (C. elegans) antibody

PAR6B antibody

PARD6 beta antibody

PARD6B antibody

partitioning defective 6 homolog beta antibody

partitioning-defective protein 6, C. elegans, homolog of, beta antibody

General Information

Product

PARD6B Antibody

Description

Affinity Purified Partitioning Defective 6 homolog beta Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 340- 372 on human Partitioning Defective 6 homolog beta.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9BYG5

Overview

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

Molecular Function

protein kinase C binding

Rho GTPase binding

Subcellular Location

Cell membrane, Cytoplasm

Expression

Expressed in pancreas and in both adult and fetal kidney. Weakly expressed in placenta and lung. Not expressed in other tissues.

Structure

The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.

The PDZ domain mediates interaction with MPP5.

Alternative Nomenclature

OTTHUMP00000031269 antibody

PAR 6 beta antibody

Par 6 partitioning defective 6 homolog beta (C. elegans) antibody

PAR6B antibody

PARD6 beta antibody

PARD6B antibody

partitioning defective 6 homolog beta antibody

partitioning-defective protein 6, C. elegans, homolog of, beta antibody

General Information

Product

Phospho-Cofilin Antibody

Description

Affinity Purified Phosphorylated Cofilin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 1-50 on human Phosphorylated Cofilin.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P23528

Overview

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity).

Molecular Function

actin filament binding

Subcellular Location

Plasma membrane, Cytoskeleton, Nucleus

Expression

Widely distributed in various tissues.

Structure

Belongs to the actin-binding proteins ADF family.

Alternative Nomenclature

18 kDa phosphoprotein antibody

CFL 1 antibody

CFL antibody

CFL1 antibody

COF1_HUMAN antibody

Cofilin 1 antibody

Cofilin 1 non muscle antibody

Cofilin antibody

Cofilin non muscle isoform antibody

Cofilin-1 antibody

epididymis secretory protein Li 15 antibody

HEL-S-15 antibody

non-muscle isoform antibody

p18 antibody