FOXP3 Antibody

General Information

Product

FOXP3 Antibody

Description

Forkhead box protein 3 Antibody

Verified Applications

ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Cat, Human, Monkey, Mouse, Rat

Predicted: Pig, Cynomolgus monkey, Rhesus monkey, Deer

Immunogen

Synthetic peptide corresponding to amino acids 229-250 of Mouse Forkhead Box Protein 3 (winged helix transcription factor 3 protein). The peptide was post synthetically modified to achieve desired antigenicity before coupling to a carrier protein.

Sequence:

AQCLLQREVVQSLEQQLELEKE

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyconal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:15,000-1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9H5F2

Overview

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.

Molecular Function

Core promoter binding; Histone Acetyltransferase binding; Histone deacetylase binding; NFAT protein binding; NF-kappaB binding; Activator; Repressor

Subcellular Location

Nucleus; Cytoplasm

Expression

Down-regulated in regulatory T-cells (Treg) during inflammation.

Structure

Homodimer. Interacts with IKZF3. Isoform 1 (via LXXLL motif), but not isoform 2, interacts with isoform 4 of RORA (via AF-2 motif). Interacts with STUB1, HSPA8 and HSPA1A/B. Interacts with PPP1CA, PPP1CB and PPP1CG. Interacts with KAT5 and HDAC7. Interacts with HDAC9 in the absence of T-cell stimulation. Interacts with USP7. Interacts with isoform 2 of ZFP90 and can form a complex with TRIM28 in the presence of isoform 2 of ZFP90. Interacts with RUNX1. Interacts with RORC. Interacts with RELA and NFATC2. Interacts with RUNX2, RUNX3 and IKZF4.

Alternative Nomenclature

AIID antibody

DIETER antibody

Forkhead box P3 antibody

FOXP3delta7 antibody

Immune dysregulation polyendocrinopathy enteropathy X linked antibody

IPEX antibody

JM2 antibody

MGC141961 antibody

MGC141963 antibody

PIDX antibody

SCURFIN antibody

XPID antibody