General Information

Product

RIPK2 Antibody Affinity Purified

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 2 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Pig, Monkey

Immunogen

Synthetic peptide taken within amino acid region 410-460 on human RIPK2 protein.

Accession #

GenBank: AAQ89173.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O43353

Overview

Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Molecular Function

ATP binding; CARD domain binding; LIM domain binding; non-membrane spanning protein tyrosine kinase activity; Protein homodimerization activity; protein serine/threonine kinase activity; signal transducer activity

Subcellular Location

Cytoplasm

Expression

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Structure

Found in a signaling complex consisting of at least ARHGEF2, NOD2 and RIPK2. Interacts with ARHGEF2; the interaction mediates tyrosine phosphorylation of RIPK2 by Src kinase CSK. Binds to CFLAR/CLARP and CASP1 via their CARD domains. Binds to BIRC3/c-IAP1 and BIRC2/c-IAP2, TRAF1, TRAF2, TRAF5 and TRAF6. May be a component of both the TNFRSF1A and TNRFSF5/CD40 receptor complex. Interacts with NOD1. Interacts (via CARD domain) with NOD2 (via CARD domain). Interacts with MAP3K4; this interaction sequesters RIPK2 from the NOD2 signaling pathway. Interacts with IKBKG/NEMO. The polyubiquitinated protein interacts with MAP3K7/TAK1. Interacts with XIAP/BIRC4. Interacts with NLRP10. Interacts with CARD9.

Alternative Nomenclature

CARD carrying kinase antibody

CARD CONTAINING ICE ASSOCIATED KINASE antibody

CARD containing interleukin 1 beta converting enzyme associated kinase antibody

CARDIAK antibody

CLARP kinase antibody

GIG 30 antibody

Growth inhibiting gene 30 antibody

Receptor interacting protein 2 antibody

Receptor interacting serine threonine kinase 2

RICK antibody

RIP-like-interacting CLARP kinase antibody

General Information

Product

RIPK1 Antibody Affinity Purified

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein kinase 1

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within aino acid region 620-670 on human Receptor-interacting Serine/Threonine-protein kinase 1 protein.

Accession #

NCBI:  NP_003795.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q13546

Overview

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Molecular Function

ATP binding; Death domain binding; Identical protein binding; Protein complex binding; Protein kinase activity; protein serine/threonine kinase activity; Ubiquitin protein ligase binding

Subcellular Location

Cytoplasm

Structure

Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif). Upon TNF-induced necrosis, the RIPK1-RIPK3 dimer further interacts with PGAM5 and MLKL; the formation of this complex leads to PGAM5 phosphorylation and increase in PGAM5 phosphatase activity. Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process. Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3. Interacts with BNLF1. Interacts with SQSTM1 upon TNF-alpha stimulation. May interact with MAVS/IPS1. Interacts with ZFAND5. Interacts with RBCK1 (By similarity). Interacts with ZBP1 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK3 and MLKL. Interacts (via kinase domain) with DAB2IP (via Ras-GAP domain); the interaction occurs in a TNF-alpha-dependent manner. Interacts with ARHGEF2. Interacts (via protein kinase domain) with RFFL; involved in RIPK1 ubiquitination. Interacts with RNF34; involved in RIPK1 ubiquitination.

Alternative Nomenclature

Cell death protein RIP antibody

Receptor (TNFRSF) interacting serine threonine kinase 1 antibody

Receptor-interacting protein 1 antibody

Serine threonine protein kinase RIP antibody

General Information

Product

Phospho-AKT1 Antibody

Description

Affinity Purified Phosphorylated Rac protein kinase-alpha Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bird, Bovine, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 430-480 on human Rac protein kinase-alpha protein.

Accession #

GenBank: AAA36539.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P31749

Overview

One of 3 serine/threonine-protein kinases (AKT1-AKT3) regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

Molecular Function

Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in prostate cancer and all human cell types.

Structure

Interacts with and phosphorylated by PDPK1.  Interacts with several proteins invovled in phosphorylation and ubiquitination

Alternative Nomenclature

AKT 1 antibody

C AKT antibody

MGC9965 antibody

MGC99656 antibody

Oncogene AKT1 antibody

PKB antibody

PKB-ALPHA antibody

PRKBA antibody

Protein Kinase B Alpha antibody

Protein kinase B antibody

Proto-oncogene c-Akt antibody

RAC Alpha antibody

RAC antibody

RAC PK Alpha antibody

RAC Serine/Threonine Protein Kinase antibody

RAC-PK-alpha antibody

vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody

General Information

Product

RIPK5 Antibody Affinity Purified

Description

Affinity Purified Dual serine/threonine and tyrosine protein kinase Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human Dual serine/threonine and tyrosine protein kinase protein (RIPK5).

Accession #

GenBank: Q6XUX3.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q6XUX3

Overview

Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. May induce both caspase-dependent apoptosis and caspase-independent cell death.

Molecular Function

ATP binding; Protein serine/threonine kinase activity; Protein tyrosine kinase activity; ATP-binding; Nucleotide-binding

Subcellular Location

Cell junction, Cell membrane, Cytoplasm, Membrane

Expression

Predominantly expressed in skeletal muscle and testis. Expressed in basolateral and apical membranes of all tubular epithelia. Expressed in thin ascending limb of the loop of Henle and the distal convoluted tubule. Expressed in all layers of transitional ureteric epithelium and in the ureteric smooth-muscle cells. Weakly expressed in heart, brain, placenta, kidney, pancreas, spleen, thymus, prostate, uterus, small intestine, white blood cells, stomach, spinal cord and adrenal gland. Is widely distributed in the CNS. Also detected in several tumor cell lines.

Structure

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Alternative Nomenclature

DSTYK antibody

Dual serine/threonine and tyrosine protein kinase antibody

Dusty PK antibody

Dusty protein kinase antibody

HDCMD38P antibody

KIAA0472 antibody

Receptor interacting protein kinase 5 antibody

Receptor interacting serine/threonine protein kinase 5 antibody

RIP homologous kinase antibody

RIPK5 antibody

SgK496 antibody

Sugen kinase 496 antibody

General Information

Product

G0/G1 switch 2 Antibody

Description

Affinity Purified Lymphocyte G0/G1 Switch protein 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 78-103 on mouse G0S2-like protein. The peptide was covalently modified to achieve desired antigenicity and conjugated to a carrier protein.

Sequence:

ALLAGGKAQEATLCSRALSLRQHAS

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.54 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500 Predicted molecular weight: 11 kDa.

Protein

Uniprot #

Q68515

Subcellular Location

Mitochondrion

Structure

Interacts directly with BCL2; which prevents the formation of the anti-apoptotic BAX-BCL2 complex.

Alternative Nomenclature

G0/G1 switch regulatory protein 2 antibody

OTTHUMP00000034644 antibody

Putative lymphocyte G0/G1 switch protein 2 antibody

RP1 28O10.2 antibody

General Information

Product

GADD34 Antibody

Description

Affinity Purified Growth arrest and DNA damage-inducible protein GADD34 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Predicted: Bovine, Monkey

Immunogen

Synthetic peptide corresponding to internal sequence of amino acids 380-400 of Human GADD34, covalently modified and coupled to a carrier protein.

Sequence:

PATGVFLKSWVYQPGEDTEEE

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:800-1:1,000

ELISA

1:800-1:1,000

Western Blot

1:500

Protein

Uniprot #

O75807

Overview

Recruits serine/threonine-protein phosphatase PP1 to dephosphorylate translation initation factor eIF2A/EIF2S1.  This reverses shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress.

Molecular Function

Apoptosis; Stress Response; Translation regulation

Subcellular Location

Endoplasmic Reticulum membrane; Peripheral membrane protein; Cytoplasmic side; Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic Side

Expression

induced by methyl methanesulfonate and ionizing irradiation.  Apoptosis is induced by interluekin 24 in melanoma cells

Structure

belongs to PPP1R15 family.

Alternative Nomenclature

Apoptosis associated protein antibody

GADD 34 antibody

Growth arrest and DNA-damage-inducible 34 antibody

Myeloid differentiation primary response protein MyD116 homolog antibody

PPP1R15A antibody

Protein phosphatase 1 regulatory (inhibitor) subunit 15A antibody

General Information

Product

IRE1A Antibody

Description

Affinity Purified Serine/Threonine-protein kinase/endoribonuclease IRE1 Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence within amino acid region 900-977 on human IRE1A protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

O75460

Overview

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Molecular Function

Hydrolase, Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type 1 membrane protein

Expression

Ubiquitously expressed. High levels observed in pancreatic tissue.

Structure

Interacts with DAB2IP (via PH domain); the interaction occurs in a ER stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds, HSPA5, a negative regulator of the unfolded protein response.

Alternative Nomenclature

Endoplasmic reticulum (ER) to nucleus signalling 1 antibody

Endoribonuclease antibody
ER to nucleus signaling 1 antibody
ERN 1 antibody
hIRE 1p antibody
Inositol requiring 1 antibody
Inositol requiring protein 1 antibody
inositol-requiring enzyme 1 antibody
IRE 1 antibody
IRE 1a antibody
IRE 1P antibody
Ire1 alpha antibody

MGC163277 antibody
MGC163279 antibody
Protein kinase/endoribonuclease antibody
RGD1559716 antibody
Serine/threonine protein kinase/endoribonuclease IRE1 antibody

General Information

Product

ITCH / AIP4 Antibody

Description

Affinity Purified ITCH protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 human ITCH protein.

Accession #

GenBank: AAH11571.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96J02

Overview

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways.

Subcellular Location

Cell membrane, cytoplasm, nucleus (Note: Associates with endocytic vesicles. May be recruited to exosomes by NDFIP1)

Expression

Widely expressed

Alternative Nomenclature

AIF4 antibody

AIP4 antibody

Atrophin 1 interacting protein 4 antibody

dJ468O1.1 antibody

E3 ubiquitin-protein ligase Itchy homolog antibody

EC 6.3.2 antibody

Itch antibody

Itchy E3 ubiquitin protein ligase antibody

NAPP1 antibody

NFE2 associated polypeptide 1 antibody

Ubiquitin protein ligase ITCH antibody

General Information

Product

Lyn Antibody

Description

Affinity Purified Tyrosine-protein kinase Lyn Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 425-525 on human Lyn protein.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody

General Information

Product

Phospho Lyn Antibody Affinity Purified

Description

Affinity Purified Tyrosine-protein kinase Lyn isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Immunogen

Phosphorylated synthetic peptide taken within amino acid region 425-525 on human Lyn protein. Phospho Y 491.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

LYN proto oncogene, Src family tyrosine kinase antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody