General Information

Product

CD146 Antibody

Description

Affinity Purified Cell Surface Glycoprotein MUC18 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Rat

Immunogen

Synthetic peptide taken within amino acid region 330-353 on rat Cell Surface Glycoprotein MUC18 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q9EPF2

Overview

lays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Detected in lung, uterus and placenta (at protein level). Detected in heart, lung, kidney, adrenal gland, intestine, testis, skeletal muscle and aorta. Detected at low levels in adult brain, in particular in brain stem and spinal cord, but also in hippocampus, olfactory bulb and striatum (at protein level).

Structure

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Alternative Nomenclature

A32 antigen antibody

CD 146 antibody

Cell surface glycoprotein MUC18 antibody

Cell surface glycoprotein P1H12 antibody

Gicerin antibody

Mcam antibody

Melanoma adhesion molecule antibody

Melanoma associated antigen A32 antibody

Melanoma associated antigen MUC18 antibody

Melanoma associated glycoprotein MUC18 antibody

Melanoma cell adhesion molecule antibody

Melanoma-associated antigen A32 antibody

Melanoma-associated antigen MUC18 antibody

MelCAM antibody

MUC 18 antibody

S endo 1 antibody

S endo 1 endothelial associated antigen antibody

General Information

Product

Phospho-Cofilin Antibody

Description

Affinity Purified Phosphorylated Cofilin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 1-50 on human Phosphorylated Cofilin.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P23528

Overview

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity).

Molecular Function

actin filament binding

Subcellular Location

Plasma membrane, Cytoskeleton, Nucleus

Expression

Widely distributed in various tissues.

Structure

Belongs to the actin-binding proteins ADF family.

Alternative Nomenclature

18 kDa phosphoprotein antibody

CFL 1 antibody

CFL antibody

CFL1 antibody

COF1_HUMAN antibody

Cofilin 1 antibody

Cofilin 1 non muscle antibody

Cofilin antibody

Cofilin non muscle isoform antibody

Cofilin-1 antibody

epididymis secretory protein Li 15 antibody

HEL-S-15 antibody

non-muscle isoform antibody

p18 antibody