General Information

Product

Pin1 Antibody

Description

Peptidyl-prolyl cis-trans isomerase Pin1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 118-168 on human peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein.

Accession #

NCBI:  XP_011526370.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western blot

1:500

Protein

Uniprot #

UPI0005D038B7

Overview

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).

Molecular Function

GTPase activating protein binding; mitogen-activated protein kinase kinase binding; peptidyl-prolyl cis-trans isomerase activity; phosphoserine binding; phosphothreonine binding

Subcellular Location

Nucleus; Nucleus speckle; Cytoplasm

Expression

The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.

Structure

Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).

Alternative Nomenclature

DOD antibody

DODO, Drosophila, homolog of antibody

FLJ40239 antibody

FLJ77628 antibody

MGC10717 antibody

NIMA interacting 1 antibody

Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody

Pin 1 antibody

PPIase Pin1 antibody

Prolyl isomerase antibody

Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody

Protein NIMA interacting 1 antibody

Rotamase Pin1 antibody

UBL5 antibody

General Information

Product

Pin1 Antibody

Description

Peptidyl-prolyl cis-trans isomerase Pin1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 or 118-168 on human peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein.

Accession #

NCBI:  XP_011526370.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.56-0.62 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

UPI0005D038B7

Overview

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins (PubMed:21497122, PubMed:22033920). Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed:16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed:15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed:17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed:22608923).

Molecular Function

GTPase activating protein binding; mitogen-activated protein kinase kinase binding; peptidyl-prolyl cis-trans isomerase activity; phosphoserine binding; phosphothreonine binding

Subcellular Location

Nucleus; Nucleus speckle; Cytoplasm

Expression

The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.

Structure

Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation (PubMed:22608923).

Alternative Nomenclature

DOD antibody

DODO, Drosophila, homolog of antibody

FLJ40239 antibody

FLJ77628 antibody

MGC10717 antibody

NIMA interacting 1 antibody

Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody

Pin 1 antibody

PPIase Pin1 antibody

Prolyl isomerase antibody

Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody

Protein NIMA interacting 1 antibody

Rotamase Pin1 antibody

UBL5 antibody

General Information

Product

Phospho-p27 KIP1 Antibody

Description

Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody Extreme C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 148-198 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.

Accession #

GenBank: AAH01971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:250

Protein

Uniprot #

Q9NYG6

Overview

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Molecular Function

Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity

Subcellular Location

Nucleus

Expression

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Structure

Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex.

Alternative Nomenclature

AA408329 antibody

AI843786 antibody

CDKN 1B antibody

CDKN 4 antibody

Cyclin Dependent Kinase Inhibitor 1B antibody

Cyclin dependent kinase inhibitor p27 antibody

KIP 1 antibody

MEN1B antibody

MEN4 antibody

p27 antibody

p27 Kip1 antibody

P27-like cyclin-dependent kinase inhibitor antibody

General Information

Product

Phospho-p27 KIP1 Antibody

Description

Affinity Purified Phosphorylated Cyclin-dependent kinase inhibitor p27kip1 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Chicken, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 143-193 on human Cyclin-dependent kinase inhibitor 1 (p27, KIP1) protein.

Accession #

GenBank: AAH01971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:250

Protein

Uniprot #

Q9NYG6

Overview

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Molecular Function

Cyclin-dependent protein serine/threonine kinase inhibitor activity, kinase activity

Subcellular Location

Nucleus

Expression

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Structure

Forms a ternary complex with CCNE1/CDK2/CDKN1B. Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187. Interacts with COPS5, subunit of the COP9 signalosome complex.

Alternative Nomenclature

AA408329 antibody

AI843786 antibody

CDKN 1B antibody

CDKN 4 antibody

Cyclin Dependent Kinase Inhibitor 1B antibody

Cyclin dependent kinase inhibitor p27 antibody

KIP 1 antibody

MEN1B antibody

MEN4 antibody

p27 antibody

p27 Kip1 antibody

P27-like cyclin-dependent kinase inhibitor antibody

General Information

Product

S100 beta Antibody Affinity Purified

Description

Affinity Purified Protein S100-P Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on human S100 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P25815

Overview

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).

Molecular Function

Calcium-dependent protein binding, calcium ion binding, magnesium ion binding

Subcellular Location

Nucleus, cytoplasm, Cell projection > microvillus membrane

Expression

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial n

Structure

Homodimer and heterodimer with S100A1. Interacts with S100PBP and S100Z. Interacts with CACYBP in a calcium-dependent manner. Dimeric form binds to and activates EZR/Ezrin by unmasking its F-actin binding sites. Interacts with PPP5C (via TPR repeats); the

Alternative Nomenclature

NEF antibody

S 100 calcium binding protein beta chain antibody

S 100 protein beta chain antibody

S 100 protein beta subunit antibody

S100 calcium-binding protein B antibody

General Information

Product

S100 beta Antibody Affinity Purified

Description

Affinity Purified Protein S100-P Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

C-epitope synthetic peptide corresponding to unique amino acid sequences on human S100 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P25815

Overview

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).

Molecular Function

Calcium-dependent protein binding, calcium ion binding, magnesium ion binding

Subcellular Location

Nucleus, cytoplasm, Cell projection > microvillus membrane

Expression

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial n

Structure

Homodimer and heterodimer with S100A1. Interacts with S100PBP and S100Z. Interacts with CACYBP in a calcium-dependent manner. Dimeric form binds to and activates EZR/Ezrin by unmasking its F-actin binding sites. Interacts with PPP5C (via TPR repeats); the

Alternative Nomenclature

NEF antibody

S 100 calcium binding protein beta chain antibody

S 100 protein beta chain antibody

S 100 protein beta subunit antibody

S100 calcium-binding protein B antibody

General Information

Product

S100 beta Antibody

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Full length protein of human S100 beta/beta homodimer.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P25815

Overview

May function as calcium sensor and contribute to cellular calcium signaling. In a calcium-dependent manner, functions by interacting with other proteins, such as EZR and PPP5C, and indirectly plays a role in physiological processes like the formation of microvilli in epithelial cells. May stimulate cell proliferation in an autocrine manner via activation of the receptor for activated glycation end products (RAGE).

Molecular Function

Calcium-dependent protein binding, calcium ion binding, magnesium ion binding

Subcellular Location

Nucleus, cytoplasm, Cell projection > microvillus membrane

Expression

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.

Structure

Homodimer and heterodimer with S100A1. Interacts with S100PBP and S100Z. Interacts with CACYBP in a calcium-dependent manner. Dimeric form binds to and activates EZR/Ezrin by unmasking its F-actin binding sites. Interacts with PPP5C (via TPR repeats); the interaction is calcium-dependent and modulates PPP5C activity.

Alternative Nomenclature

NEF antibody

S 100 calcium binding protein beta chain antibody

S 100 protein beta chain antibody

S 100 protein beta subunit antibody

S100 calcium-binding protein B antibody

General Information

Product

Saxophone Antibody Affinity Purified

Description

Affinity Purified Serine/Threonine kinase receptor SAX (sax) mRNA, complete cds Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequence near C-terminal of Saxophone protein.

Accession #

GenBank: U11441.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q7JPM7

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase

Alternative Nomenclature

CG1891 antibody

receptor protein serine/threonine kinase antibody

saxophone antibody

X linked surface antigen antibody

General Information

Product

Saxophone Antibody

Description

Affinity Purified Serine/Threonine kinase receptor SAX (sax) Antibody N-epitope

Verified Applications

ELISA, ICC, IHC-FoFr, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Synthetic peptide corresponding to amino acids 107-125 of D. melanogaster SAX protein. Peptide was conjugated to KLH.

Sequence:

GCSTSPDQLPMICSQNSLK

Accession #

GenBank: U11441.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.2 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q7JPM7

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase

Alternative Nomenclature

CG1891 antibody

receptor protein serine/threonine kinase antibody

saxophone antibody

X linked surface antigen antibody

General Information

Product

LYK5 Antibody

Description

Affinity Purified STE20-related kinase adapter protein alpha Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 381-431 on human STE-20 related kinase adapter protein alpha.

Accession #

NCBI:  NP_001003787.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q7RTN6

Overview

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Molecular Function

ATP Binding, protein kinase activator activity, kinase binding, transferase activity, transferring phosphorus-containing groups

Subcellular Location

Nucleus, cytoplasm

Expression

Expressed in liver

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 protein kinase domain.

Alternative Nomenclature

Protein kinase LYK5 antibody

Serologically defined breast cancer antigen NY BR 96 antibody

STE20 related adapter protein antibody

STRAD alpha antibody

STRAD antibody