General Information
|
|
Product
|
PDE4D5 Antibody
|
Description
|
Affinity Purified cAMP-specific phosphodiesterase PDE4D5 Antibody
|
Verified Applications
|
CM, ELISA, ICC, IF, IHC, IP, WB
|
Host
|
Rabbit
|
Species Cross Reactivity
|
Human, Mouse, Rabbit, Rat
|
Immunogen
|
A 20 amino acid synthetic peptide taken from the N-terminus corresopnding to the PDE4D5 variant.
|
Specificity |
A This antibody does not cross-react with other members of the PDE4D family or with other PDE4 proteins (PDE4A, PDE4B and PDE4C) or with other PDE family members. |
Accession #
|
GenBank: AAC00069.1
|
Physical Properties
|
|
Quantity
|
100 µg
|
Volume
|
200 µl
|
Concentration
|
0.65 µg/µl in antibody stabilization buffer
|
Form
|
Affinity Purified
|
Clonality
|
Polyclonal
|
Storage
|
-20⁰C for long term storage
|
Application Protocol
|
Confocal Microscopy
|
1:200
|
DB
|
1:10,000
|
ELISA
|
1:10,000
|
Immunocytochemistry
|
1:200
|
Immunofluorescence
|
1:200
|
Immunohistochemistry
|
1:200
|
Immunoprecipitation
|
1:150
|
Western Blot
|
1:500
|
Protein
|
Uniprot #
|
Q08499
|
Overview
|
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
|
Molecular Function
|
Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
|
Subcellular Location
|
Apical cell membrane, Cytoplasm, Membrane
|
Expression
|
Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.
|
Structure
|
Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphotase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.
|
Alternative Nomenclature
|
cAMP-specific 3'5'-cyclic phosphodiesterase 4D antibody
DPDE3 antibody
dunce-like phosphodiesterase E3 antibody
hPDE4D5 antibody
PDE4D antibody
PDE4DN2 antibody
Phosphodiesterase 4D antibody
Phosphodiesterase 4D cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) antibody
Phosphodiesterase 4D cAMP-specific antibody
STRK1 antibody
|
|
|