OGT1 Antibody

Product no.: OGT1-101AP
$285.00


General Information

 

 

Product

 

OGT1 Antibody

Description

Affinity Purified O-linked N-acetylglucosamine transferase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 980-1030 on human O-linked N-acetylglucosamine transferase protein.


Physical Properties

 

 

Quantity

 

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20C for long term storage


Application Protocol

 

ELISA

 

1:10,000

Immunohistochemistry 1:50-1:100

Immunoprecipitation

1:150

Western Blot

1:500


Protein

 

Uniprot #

 

O15294

Overview

Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1. Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity.

Molecular Function

Chromatin regulator, Glycosyltransferase, Transferase

Subcellular Location

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus

Expression

Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver.

Structure

The TPR repeat domain is required for substrate binding and oligomerization.

Alternative Nomenclature

GlcNAc transferase

HRNT1 

O GlcNAc 

O GlcNAc transferase p110 subunit 

O linked N acetylglucosamine (GlcNAc) transferase (UDP N acetylglucosamine:polypeptide N acetylglucosaminyl transferase) 

UDP N acetylglucosamine peptide N

UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit 

   

Protein information supplied by UniProt 

 

Accessories

Product Note Status Price
OGT1 Antibody BIOTIN OGT1 Antibody BIOTIN
OGT1-BIOTIN $375.00 *
OGT1 Antibody FITC OGT1 Antibody FITC
OGT1-FITC $375.00 *
OGT1 Blocking Peptide OGT1 Blocking Peptide
P-OGT1 $155.00 *
OGT1 Positive Control OGT1 Positive Control
PC-OGT1 $200.00 *
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