General Information |
|
Product |
TRF2 Antibody BIOTIN-Conjugated |
Description |
BIOTIN-Conjugated Telomeric Repeat-binding Factor 2 Antibody C-epitope |
Verified Applications |
ELISA, IHC, IP, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Monkey |
Immunogen |
Synthetic peptide taken within amino acid region 360-410 on human Telomeric repeat-binding factor 2 protein. |
Accession # |
GenBank: AAB81135.1 |
|
|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.50 µg/µl in antibody stabilization buffer |
Form |
BIOTIN-Conjugated |
Clonality |
Polyclonal |
Determinant |
C-epitope |
Storage |
-20⁰C for long term storage |
|
|
DB |
1:20,000 |
ELISA |
1:20,000 |
Immunohistochemistry | 1:100 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500-1:750 |
|
|
Uniprot # |
|
Overview |
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length. |
Molecular Function |
Chromatin binding; Double-stranded telomeric DNA binding; Protein C-terminus binding; Protein homodimerization activity; Telomeric DNA binding |
Subcellular Location |
Nucleus; Chromosome > telomere |
Expression |
Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. |
Structure |
Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct. |
Alternative Nomenclature |
Telomeric DNA binding protein antibody Telomeric repeat binding factor 2 antibody TERF 2 antibody TRBF 2 antibody TRF 2 antibody TTAGGG repeat-binding factor 2 antibody |
Protein information supplied by UniProt
Product | Note | Status | Price | |
---|---|---|---|---|
|
P-TRF2.221 | |||
|
TRF2-221AP | |||
|
TRF2.221-FITC | |||
|
PC-TRF2 | |||
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