General Information
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Product
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KIRREL Antibody BIOTIN
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Description
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BIOTIN-Conjugated Kin of IRRE-like protein 1 Antibody N-epitope
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Verified Applications
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CM, ELISA, IF, IHC, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Mouse, Rat
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Immunogen
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Synthetic peptide taken within amino acid region 200-250 on mouse Neph1 protein.
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Specificity |
Extracellular domain. |
Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.45-0.65 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Determinant
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N-epitope
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Storage
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-20⁰C for long term storage
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Application Protocol
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Confocal Microscopy
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1:250
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DB
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1:10,000
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ELISA
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1:10,000
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Immunocytochemistry
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1:250
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Immunofluorescence
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1:250
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Immunohistochemistry
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1:250
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Immunoprecipitation
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1:250
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Western Blot
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1:250
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Protein
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Uniprot #
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Q80W68
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Overview
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Plays a significant role in the normal development and function of the glomerular permeability. Is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1.
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Molecular Function
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Myosin Binding
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Subcellular Location
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Cell membrane; Single-pass type I membrane protein
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Expression
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Abundantly expressed in kidney. Specifically expressed in podocytes of kidney glomeruli.
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Structure
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Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylate
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Alternative Nomenclature
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FLJ10845 antibody
Kin of IRRE like (Drosophila) antibody
Kin of IRRE like antibody
Kin of irregular chiasm-like protein 1 antibody
Kirrel antibody
MGC129542 antibody
MGC129543 antibody
NEPH 1 antibody
Nephrin like protein 1 antibody
Nephrin related antibody
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