General Information

Product

LYK5 Antibody

Description

Affinity Purified STE20-related kinase adapter protein alpha Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human STE-20 related kinase adapter protein alpha.

Accession #

NCBI:  NP_001003787.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q7RTN6

Overview

Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.

Molecular Function

ATP Binding, protein kinase activator activity, kinase binding, transferase activity, transferring phosphorus-containing groups

Subcellular Location

Nucleus, cytoplasm

Expression

Expressed in liver

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 protein kinase domain.

Alternative Nomenclature

Protein kinase LYK5 antibody

Serologically defined breast cancer antigen NY BR 96 antibody

STE20 related adapter protein antibody

STRAD alpha antibody

STRAD antibody

General Information

Product

B-raf Antibody

Description

Affinity Purified Serine/threonine-protein kinase B-raf Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human serine/threonine-protein kinase B-raf protein.

Accession #

NCBI:  NP_004324.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500. Molecular Weight: 84.4

Protein

Uniprot #

P15056

Overview

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Nucleus, Cytoplasm, Cell membrane

Expression

Brain and testis.

Structure

Monomer. Homodimer. Heterodimerizes with RAF1, and the heterodimer possesses a highly increased kinase activity compared to the respective homodimers or monomers. Heterodimerization is mitogen-regulated and enhanced by 14-3-3 proteins. MAPK1/ERK2 activation can induce a negative feedback that promotes the dissociation of the heterodimer by phosphorylating BRAF at Thr-753. Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3 and RGS14. Interacts with RIT1. Interacts (via N-terminus) with RGS14 (via RBD domains); the interaction mediates the formation of a ternary complex with RAF1, a ternary complex inhibited by GNAI1. Interacts with DGKH. Interacts with PRMT5. Interacts with KSR2.

Alternative Nomenclature

94 kDa B raf protein antibody

B raf 1 antibody

B Raf proto oncogene serine threonine protein kinase antibody

B RAF1 antibody

B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody

BRAF 1 antibody

cRmil antibody

Murine sarcoma viral v raf oncogene homolog B1 antibody

NS7 antibody

Oncogen BRAF antibody

p94 antibody

Proto-oncogene B-Raf antibody

Proto-oncogene c-Rmil antibody

RAFB1 antibody

RMIL antibody

Serine/threonine-protein kinase B-raf antibody

v-Raf murine sarcoma viral oncogene homolog B1 antibody

General Information

Product

MAPK12 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 12 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 310-360 on human Mitogen-activated protein kinase 12.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P53778

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK12 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Mitochondrion, Nucleus

Expression

Highly expressed in skeletal muscle and heart.

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

ERK 3 antibody
ERK 5 antibody
ERK 6 antibody=Extracellular signal-regulated kinase 6 antibody
MAP kinase 12 antibody
MAP kinase p38 gamma antibody

Mitogen Activated Protein Kinase 12 antibody
Mitogen activated protein kinase 3 antibody

P38 GAMMA antibody

PRKM12 antibody
SAPK 3 antibody

Stress Activated Protein Kinase 3 antibody

General Information

Product

MAPK13 Antibody

Description

Affinity Purified Mitogen-activated protein kinase 13 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 310-360 on human Mitogen-activated protein kinase 13.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O15264

Overview

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytosol

Expression

Expressed in testes, pancreas, small intestine, lung and kidney. Abundant in macrophages, also present in neutrophils, CD4+ T-cells, and endothelial cells.

Structure

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Alternative Nomenclature

MAP kinase 13 antibody

MAPK 13 antibody

Mitogen activated protein kinase 13 antibody
p38 delta antibody

PRKM13 antibody
SAPK 4 antibody
Stress activated protein kinase 4 antibody

General Information

Product

Phospho-JAK2 Antibody

Description

Affinity Purified Phosphorylated Tyrosine-protein kinase JAK2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 980-1030 on human JAK2 protein. Phosphorylated 1008 & 1009 tyrosine residues.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

O60674

Overview

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor.

Molecular Function

Adaptive immunity, Immunity, Innate immunity

Subcellular Location

Endomembrane system; Peripheral membrane protein; Cytoplasm; Nucleus

Expression

Ubiquitously expressed throughout most tissues.

Structure

Possesses 2 protein kinase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for protein kinase 1.

Alternative Nomenclature

JAK 2 

Janus Activating Kinase 2 

Janus kinase 2 (a protein tyrosine kinase) 

Janus kinase 2 

JTK 10 

THCYT3 

Tyrosine protein kinase JAK2 

General Information

Product

CCAR1 Antibody

Description

Affinity Purified Cell division cycle and apoptosis regulator protein 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 645-695 on human Cell division cycle and apoptosis regulator protein 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q8IX12

Overview

Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation (PubMed:12816952). In association with CALCOCO1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781). Can act as a both a coactivator and corepressor of AR-mediated transcription. Contributes to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites. May play an important role in the growth and tumorigenesis of prostate cancer cells (PubMed:23887938).

Molecular Function

Activator, Repressor

Subcellular Location

Cytoplasm > perinuclear region

Expression

Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.

Structure

Contains 1 SAP domain. Coiled coil.

Alternative Nomenclature

Carp1 antibody
Ccar1 antibody
Cell division cycle and apoptosis regulator protein 1 antibody
Death inducer with SAP domain antibody
RP11-437A18.1 antibody

General Information

Product

ARMET Antibody

Description

Affinity Purified Mesencephalic astrocyte-derived neurotrophic factor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 80-120 on human Mesencephalic astrocyte-derived neurotrophic factor.

Accession #

NCBI:  NP_006001

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P55145

Overview

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons. Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death.

Molecular Function

Growth factor

Subcellular Location

Secreted, Endoplasmic reticulum, Extracellular space, Nucleus

Expression

By endoplasmic reticulum stress.

Structure

The N-terminal region may be responsible for neurotrophic activity while the C-terminal region may play a role in the ER stress response.

Alternative Nomenclature

Arginine rich mutated in early stage tumors antibody
Arginine rich protein antibody
ARMET antibody
ARP antibody
Manf antibody
Mesencephalic astrocyte derived neurotrophic factor antibody

General Information

Product

Von Hippel Lindau Antibody

Description

Affinity Purified Von Hippel-Lindau disease tumor suppressor Antibody

Verified Applications

ELISA, IHC, IP, WB

Immunogen

Synthetic peptide taken within amino acid region 130-200 on human Von Hippel-Lindau disease tumor suppressor protein.

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P40337

Overview

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.

Molecular Function

Enzyme binding, Transcription factor binding, Ubiquitin protein ligase activity,

Subcellular Location

Cytoplasm, Peripheral membrane protein, nucleus

Expression

Expressed in the adult and fetal brain and kidney.

Structure

Belongs to the VHL family.

Alternative Nomenclature

Elongin binding protein antibody

G7 protein antibody

HRCA 1 antibody

Protein G7 antibody

pVHL antibody

RCA 1 antibody

VHL 1 antibody

VHLH antibody

Von Hippel Lindau disease tumor suppressor antibody

von Hippel Lindau syndrome antibody

von Hippel Lindau tumor suppressor antibody

Von Hippel Lindau tumor suppressor, E3 ubiquitin protein ligase antibody

General Information

Product

Deformed Epidermal Autoregulatory Factor 1 Antibody

Description

Affinity Purified Deformed epidermal autoregulatory factor 1 homolog Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 500-565 on mouse Deformed epidermal autoregulatory factor 1 homolog protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Z1T5

Overview

Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction. When secreted, behaves as an inhibitor of cell proliferation, by arresting cells in the G0 or G1 phase. Required for neural tube closure and skeletal patterning. Regulates epithelial cell proliferation and side-branching in the mammary gland. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 4. Isoform 4 may inhibit transcriptional activity of isoform 1 by interacting with isoform 1 and retaining it in the cytoplasm. Transcriptional activator of EIF4G3.

Molecular Function

DNA binding, metal ion binding, transcription factor activity, sequence-specific DNA binding

Subcellular Location

Cytoplasm, Nucleus, Secreted

Expression

Expressed in various tissues and cells such as in peripheral mononuclear cells and hormone-secreting pituitary cells. Expression in pancreatic lymph nodes of patients with type 1 diabetes is 20 times higher than in healthy controls. Highly expressed in fetal and adult brain.

Structure

Zinc-finger

Alternative Nomenclature

Deaf1 antibody

DEAF1 transcription factor antibody

Deformed epidermal autoregulatory factor 1 homolog antibody

Nuclear DEA1 related transcriptional regulator antibody

NUDR antibody

SPN antibody

Suppressin antibody

Zinc finger MYND domain containing protein 5 antibody

ZMYND5 antibody

General Information

Product

THAP1 Antibody

Description

Affinity Purified THAP domain-containing protein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Predicted: Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-210 on human THAP domain-containing protein 1.

Accession #

GenBank: CAG33537

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9NVV9

Overview

DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis.

Molecular Function

Identical protein binding, Sequence-specific DNA binding, Zinc ion binding

Subcellular Location

Nucleus > nucleoplasm, PML body

Expression

Highly expressed in heart, skeletal muscle, kidney and liver. Weaker expression in brain and placenta.

Structure

Belongs to the THAP1 family.

Alternative Nomenclature

Nuclear proapoptotic factor antibody

THAP 1 antibody

THAP domain containing apoptosis associated protein 1 antibody

THAP domain containing protein 1 antibody

THAP domain protein 1 antibody