General Information |
|
Product |
Myocilin Antibody FITC |
Verified Applications |
CM, ELISA, ICC/IF, IHC, IP, Immunomicroscopy, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Monkey, Mouse, Rat |
Immunogen |
Synthetic peptide corresponding to N terminal amino acids 25-46 of Myocilin. Peptide was covalently modified and conjugated to KLH.
Sequence: CLWDVGARTAQLRKANDQSGR |
Accession # |
GenBank: BAA24532.1 |
|
|
Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.75 µg/µl in antibody stabilization buffer |
Conjugate | FITC. Ex: 493 nm, Em: 528 nm |
Clonality |
Polyclonal |
Storage |
-20⁰C for long term storage |
|
|
Confocal Microscopy |
1:200 |
DB |
1:10,000 |
ELISA |
1:10,000 |
Immunocytochemistry |
1:200 |
Immunofluorescence |
1:200 |
Immunohistochemistry |
1:200 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500 |
|
|
Uniprot # |
|
Overview |
Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction. |
Molecular Function |
fibronectin binding, myosin light chain binding, frizzled binding |
Subcellular Location |
Secreted, Golgi apparatus, Cytoplasmic vessicle |
Expression |
Expressed in large amounts in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart, bone marrow-derived mesenchymal stem cells and other tissues. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and appeared more intensively than in normal eyes, regardless of the type or clinical severity of glaucoma. |
Structure |
Homodimer (via N-terminus). Interacts with OLFM3, FN1, NRCAM, GLDN and NFASC. Interacts (via N-terminus) with MYL2. Interacts with SFRP1, FRZB, FZD7, FZD10, FZD1 and WIF1; regulates Wnt signaling. Interacts with SNTA1; regulates muscle hypertrophy. Interacts with ERBB2 and ERBb3; activates ERBB2-ERBB3 signaling pathway. Interacts with SNCG; affects its secretion and aggregation. |
Alternative Nomenclature |
Glaucoma 1 open angle antibody GLC1A antibody GPOA antibody JOAG antibody Mutated trabecular meshwork-induced glucocorticoid response protein antibody MYOC antibody Myocilin antibody TIGR antibody |
Protein information supplied by UniProt
Product | Note | Status | Price | |
---|---|---|---|---|
|
MYO-101AP | |||
|
P-MYO101 | |||
|
MYO101-BIOTIN | |||
|
PC-MYO | |||
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