General Information

Product

Apelin Receptor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-377 rat Apelin receptor protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9JHG3

Overview

Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity and plays a role in various processes in adults such as regulation of blood pressure, heart contractility, and heart failure. Also plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.

Molecular Function

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane, Multi-pass membrane protein

Expression

Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.

Structure

Transmembrane, Transmembrane helix

Alternative Nomenclature

AGTRL1 ligand antibody

APEL antibody

Apelin-13 antibody

APJ endogenous ligand antibody

Apln antibody

XNPEP2 antibody

General Information

Product

Apelin Receptor Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 human Apelin receptor protein.

Accession #

NCBI:  NP_005152

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P35414

Overview

Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity and plays a role in various processes in adults such as regulation of blood pressure, heart contractility, and heart failure. Also plays a key role in early development such as gastrulation and heart morphogenesis by acting as a receptor for APELA hormone. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.

Molecular Function

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Subcellular Location

Cell membrane, Multi-pass membrane protein

Expression

Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.

Structure

Transmembrane, Transmembrane helix

Alternative Nomenclature

AGTRL1 ligand antibody

APEL antibody

Apelin-13 antibody

APJ endogenous ligand antibody

Apln antibody

XNPEP2 antibody

General Information

Product

CD300 Antibody

Description

Affinity Purified CMRF35-like molecule 1 / CD300LF Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 230-290 on human CMRF35-like molecule 1 / CD300LF.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8TDQ1

Overview

Acts as an inhibitory receptor for myeloid cells and mast cells (PubMed:15549731). Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands (PubMed:24035150). May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:22043923). Inhibits osteoclast formation. Induces macrophage cell death upon engagement.

Molecular Function

ceramide binding

interleukin-4 receptor binding

phosphatidylserine binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells.

Alternative Nomenclature

CD300 antigen like family member antibody

CD300 antigen-like family member F antibody

CD300f antibody

CD300LF antibody

CLM antibody

CLM-1 antibody

CMRF35-like molecule 1 antibody

IGSF antibody

IgSF13 antibody

Immune receptor expressed on myeloid cells 1 antibody

immunoglobin superfamily member antibody

Immunoglobulin superfamily member 13 antibody

Inhibitory receptor IREM1 antibody

IREM antibody

IREM-1 antibody

Nepmucin antibody

NK inhibitory receptor antibody

NKIR antibody

TREM antibody

triggering receptors expressed on myeloid cells antibody

UNQ3105/PRO10111 antibody

General Information

Product

DARPP32 Antibody

Description

Affinity Purified Protein phosphatase 1 regulatory subunit 1B / PPR1B Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-80 on human Protein phosphatase 1 regulatory subunit 1B.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9UD71

Overview

Inhibitor of protein-phosphatase 1.

Molecular Function

cAMP-dependent protein kinase inhibitor activity

D1 dopamine receptor binding

D2 dopamine receptor binding

D3 dopamine receptor binding

D4 dopamine receptor binding

D5 dopamine receptor binding

protein kinase inhibitor activity

protein phosphatase inhibitor activity

Subcellular Location

Cytoplasm, cytosol, neuronal cell body, nucleus

Structure

Belongs to the protein phosphatase inhibitor 1 family.

Alternative Nomenclature

DARPP 32 antibody

Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody

Dopamine and cAMP regulated neuronal phosphoprotein antibody

Dopamine and cAMP regulated phosphoprotein DARPP32 antibody

IPPD antibody

Neuronal phosphoprotein DARPP 32 antibody

PPP1R1B antibody

Protein phosphatase 1 regulatory inhibitor subunit 1B antibody

Protein phosphatase 1 regulatory subunit 1B antibody

General Information

Product

PERK Antibody

Description

Affinity Purified Eukaryotic translation initiation factor 2-alpha kinase 3 Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on human Eukaryotic translation initiation factor 2-alpha kinase 3 protein.

Accession #

NCBI:  NP_004827

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9NZJ5

Overview

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Expression

Ubiquitous. A high level expression is seen in secretory tissues.

Structure

Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3 and MFN2.

Alternative Nomenclature

Eif2ak3 antibody

Eukaryotic translation initiation factor 2 alpha kinase 3 antibody

Heme regulated EIF2 alpha kinase antibody

HRI antibody

HsPEK antibody

Pancreatic eIF2 alpha kinase antibody

PEK antibody

PRKR like endoplasmic reticulum kinase antibody

WRS antibody

General Information

Product

P70 S6 Kinase beta Antibody

Description

Affinity Purified Ribosomal protein S6 kinase beta Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human Ribosomal protein S6 kinase beta protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P23443

Molecular Function

ATP binding

kinase activity

protein kinase activity

protein serine/threonine/tyrosine kinase activity

protein serine/threonine kinase activity

ribosomal protein S6 kinase activity

Subcellular Location

Cell junction, Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus, Synapse, Synaptosome

Expression

Widely expressed

Structure

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Alternative Nomenclature

70 kDa ribosomal protein S6 kinase 2 antibody
KS6B2_HUMAN antibody
p70 beta antibody
p70 ribosomal S6 kinase beta antibody
p70 S6 kinase beta antibody
p70 S6K-beta antibody
p70 S6KB antibody
p70-beta antibody
p70-S6K 2 antibody
Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
Ribosomal protein S6 kinase B2 antibody
Rps6kb2 antibody
S6 kinase related kinase antibody
S6K beta 2 antibody
S6K beta antibody
S6K2 antibody
Serine/threonine protein kinase 14 beta antibody
STK14B antibody