General Information

Product

Adiponectin Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Pig

Immunogen

Synthetic peptide taken within amino acid region 150-243 on pig Adiponectin protein.

Accession #

GenBank: AY589691

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.65 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q69907

Overview

Involved in brown fat cell differentiation, responses to drugs, detection of oxidative stress glucose homeostasis and fatty acid oxidation.  Invovled in regulation of DNA biosynthetic processes.

Molecular Function

Hormone activity; receptor binding

Subcellular Location

Cell periphery; collagen trimer; extracellular space; cell surface; endoplasmic reticulum; perinuclear region of cytoplasm

Expression

Secreted

Alternative Nomenclature

30 kDa adipocyte complement related protein antibody

ACDC antibody

ACRP 30 antibody

Adipocyte antibody

Adipocyte C1q and collagen domain containing protein antibody

Adipocyte complement related 30 kDa protein antibody

adipocyte-specific secretory protein antibody

Adiponectin antibody

adiponectin, C1Q and collagen domain containing antibody

AdipoQ antibody

Adipose most abundant gene transcript 1 antibody

Adipose specific collagen like factor antibody

ADIPQTL1 antibody

ADPN antibody

APM 1 antibody

C1q and collagen domain-containing protein antibody

GBP 28 antibody

Gelatin binding protein antibody

Gelatin binding protein 28 antibody

General Information

Product

MC4 Receptor Antibody

Description

Affinity Purified Melanocortin receptor 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 10-60 on human MC4R protein.

Accession #

NCBI:  NP_005903.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P32245

Overview

Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP).

Molecular Function

Melanocortin receptor activity, melanocyte-stimulating hormone receptor activity, neuropeptide binding, ubiquitin protein ligase binding

Subcellular Location

cell membrane, multi-pass membrane protein, membrane raft. (Note: Localizes to membrane rafts but is also loated in the cell membrane outside of these regions where channel response to cold is enhanced compared to membrane rafts).

Expression

Brain, placental, and gut tissues.

Alternative Nomenclature

MC4 R antibody

Melanocortin receptor 4 antibody

MGC126851 antibody

MGC138197 antibody

General Information

Product

5HT1B Receptor Antibody

Description

Affinity Purified 5 Hydroxytryptamine 1B receptor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 160-260 on rat 5HT1B

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:50,000

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P28334

Overview

G-protein coupled receptor for serotonin (5-hydroxytryptamine).   Functions as a receptor for various drugs which cause conformational change upon ligand binding that triggers signaling via guanine nucleo-tide binding proteins and modulation of down-stream effectors like adenylate cyclase.  Inhibits adenylate cyclase activity and activates phosphatidylinositol-calcium second messenger system that regulates release of intracellular Calcium ions.

Molecular Function

Drug binding, serotonin receptor activity, serotonin binding

Subcellular Location

Cell membrane; Multi-pass protein

Structure

Homodimer. Heterodimer with HTR1D

Alternative Nomenclature

HTR1DB antibody

S12 antibody

Serotonin 1D beta receptor antibody

General Information

Product

ACTH Antibody

Description

Affinity Purified Adrenocorticotropin Hormone Antibody

Verified Applications

ELISA, IHC-Fr, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Predicted: Chicken, Chimpanzee, Cow, Dog, Goat, Guinea pig, Macaque Monkey, Orangutan, Sheep

Immunogen

Synthetic peptide corresponding to ACTH aa 138-176. 

Sequence:

SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF

Accession #

NCBI:  AAX36901

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Immunocytochemistry

1:250

Immunofluorescence

1:200

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:1000 Predicted Molecular weight: 29 kDa

Protein

Uniprot #

UPI00004EBE53

Overview

Stimulates adrenal glands and release cortisol.

Molecular Function

GPCR; Hormone activity

Subcellular Location

Secreted

Expression

ACTH and MSH are produced by the pituitary gland

Structure

Belongs to POMC family

Alternative Nomenclature

Alpha Melanocyte Stimulating Hormone antibody

Beta Endorphin antibody

Beta Lipotropin antibody

Beta Melanocyte Stimulating Hormone antibody

Beta-endorphin antibody

CLIP antibody

Corticotropin Like Intermediary Peptide antibody

Met-enkephalin antibody

MSH antibody

NPP antibody

Opiomelanocortin prepropeptide antibody

POC antibody

POMC antibody

Tetracosactide antibody

General Information

Product

CCK4 / PTK7 Antibody

Description

Affinity Purified Protein Tyrosine Kinase 7 Antibody

Verified Applications

ELISA, ICC/IF, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Purified Protein tyrosine kinase 7 protein was emulsified in complete adjuvant and used to immunize the rabbits. 

Accession #

NCBI:  AAH50050

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.59 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal. The anti-serum was pre-cleared for tag protein and then affinity purified on immobilized PTK7 affinity chromatography.

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q12852

Overview

Activator of JNK/SAPK pathway.  Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Molecular Function

ATP-binding, magnesium, Nucleotide-binding.

Subcellular Location

Cytoplasm, Membrane, Peripheral membrane protein

Expression

Highly expressed in brain and kidney.

Structure

Interacts with MBIP.

Alternative Nomenclature

Colon carcinoma kinase 4 antibody

Inactive tyrosine-protein kinase 7 antibody

mPTK7/CCK4 antibody

Serum response factor antibody

Srf antibody

General Information

Product

PTGER3 Antibody

Description

Protaglandin Receptor EP3D Antibody

Verified Applications

CM, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on EP3 Receptor protein.

Accession #

NCBI:  XP_009209142.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.6 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

UPI0004F2137B

Overview

Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium. The various isoforms have identical ligand binding properties but can interact with different second messenger systems (By similarity).

Molecular Function

Prostaglandin E receptor activity, G-protein coupled receptor

Subcellular Location

Cell membrane; Multi-pass membrane

Expression

Expressed in small intestine, heart, pancreas, gastric fundic mucosa, mammary artery and pulmonary vessels.

Structure

Belongs to the G-protein coupled receptor 1 family.

Alternative Nomenclature

EP 3 antibody

EP3 I antibody

EP3 II antibody

EP3 III antibody

EP3 IV antibody

Ep3 prostanoid receptor antibody

Ep3 receptor antibody

EP3 subtype antibody

EP3e antibody

MGC141828 antibody

MGC141829 antibody

PGE receptor EP3 subtype antibody

PGE2 R antibody

Pgerep3 antibody

Prostaglandin E receptor EP3 subtype 3 isoform antibody

Prostanoid EP3 receptor antibody

PTGER 3 antibody

Rep3 antibody

rEP3a antibody

rEP3b antibody

General Information

Product

DDIT3 Antibody

Description

Affinity Purified DNA damage-inducible transcript 3 protein Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 60-160 on human GADD153 protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P35638

Overview

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes.

Molecular Function

Activator, Repressor, DNA binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Heterodimer. Interacts with TCF7L2/TCF4, EP300/P300, HDAC1, HDAC5 and HDAC6. Interacts with TRIB3 which blocks its association with EP300/P300. Interacts with FOXO3, CEBPB and ATF4.

Alternative Nomenclature

C/EBP homologous protein antibody

C/EBP zeta antibody

CCAAT/enhancer binding protein homologous protein antibody

CEBPZ antibody

CHOP 10 antibody

DDIT3 antibody

DNA Damage Inducible Transcript 3 antibody

GADD153 antibody

Growth Arrest and DNA Damage Inducible Protein 153 antibody

MGC4154 antibody

General Information

Product

mTOR Antibody Affinity Purified

Description

Affinity Purified Serine/threonine-protein kinase mTOR Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 430-480 on mTOR protein.

Accession #

NCBI:  NP_063971.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P42346

Overview

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Lysosome, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Alternative Nomenclature

FK506 binding protein 12 rapamycin associated protein 1 antibody

FKBP rapamycin associated protein antibody

FRAP1 antibody

FRAP2 antibody

mTOR antibody

RAFT1 antibody

Rapamycin target protein antibody

RAPT1 antibody

Serine/threonine-protein kinase mTOR antibody

General Information

Product

KAT13C / NCOA2 Antibody Affinity Purified

Description

Affinity Purified Nuclear receptor coactivator 2 isoform b Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid 1375-1425 on rat NuCR2/GRIP-1/SRC-2 protein.

Accession #

NCBI:  NP_001071163.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Alternative Nomenclature

bHLHe75 antibody

Class E basic helix-loop-helix protein 75 antibody

glucocorticoid receptor interacting protein 1 antibody

GRIP 1 antibody

hTIF2 antibody

MED1 antibody

MGC138808 antibody

NCoA 2 antibody

Oncogene FGR antibody

p160 antibody

SRC 2 antibody

TIF2 antibody