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ARF1 Antibody

Product no.: ARF1-101AP

$320.00

General Information
Product	ARF1 Antibody
Description	Affinity Purified ADP-ribosylation factor 1 Antibody
Verified Applications	ELISA, ICC/IF, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on Rat ARF1 protein. The peptide was covalently modified post-synthetically to achieve desied antigenicity.
Specificity	does not cross react to other members of the Ras family or to other ARF members. This antibody will label ARF isoform 1, 2, 3, and 4.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.58-0.65 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:100
Immunocytochemistry	Use at an assay dependent concentration.
Western Blot	1:500. Predicted Molecular Weight 21 kDa
Protein
Uniprot #	P84079
Overview	GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.
Molecular Function	ADP-ribosyltransferase; GTP binding; ER-Golgi transport
Subcellular Location	Gogli apparatus
Expression	Cell junction; Membrane; Synapse
Structure	Belongs to the small GTPase superfamily, Arf family
Alternative Nomenclature	DJ133P16.1 antibody

Protein information supplied by UniProt

Fabgennix ARF1 Antibody has been used in 1 Publication

Lin, Lin et al. “The Antibiotic Gentamicin Inhibits Specific Protein Trafficking Functions of the Arf1/2 Family of GTPases.” Antimicrobial Agents and Chemotherapy 55.1 (2011): 246–254. PMC3019663

Western Blot; Rat

Publishing research using ARF1-101AP? Please let us know, we can cite the reference in this datasheet.

Customer ratings for ARF1 Antibody

Number of ratings: 2
Average rating: 4.5

Good
from Verified Customer on 12/11/2020

Immunoprecipitation preformed using rat whole brain tissue lysate. 1000 ug total protein input. 3.5 ul/mg lysate incubated for 17 hours at 4C. Able to get the antibody to work well for tissue lysate, but was unable to work in ell lysates.

Excellent
from Anonymous on 11/14/2015

Immunocytochemistry/Immunofluorescence
Human Cell (Neuroblastoma) Paraformaldehyde
Permeabilization-15 mins in 0.01% Triton X
Serum as blocking agent for 30 minutes
1:100 antibody dilution for 2 hours at 20C

All incoming ratings are posted by actual shop visitors / webshop customers and their content is checked by the operator by means of a visual inspection.

Accessories

Product

Note

Status

Price

ARF1 Antibody FITC

ARF1-FITC

$375.00 *

ARF1 Antibody BIOTIN

ARF1-BIOTIN

$375.00 *

ARF1 Blocking Peptide

P-ARF1

$155.00 *

ARF1 Positive Control

PC-ARF1

$200.00 *

General Information
Product	ARF4 Antibody
Description	Affinity Purified ADP-ribosylation factor 4 Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide corresponding to unique amino acid sequence on ARF4 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.71-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	P61751
Overview	GTP-binding protein that functions as an allosteric activator of cholera toxin catalytic subunt.
Molecular Function	ADP-ribosyltransferase; GTP binding; ER-Golgi transport
Subcellular Location	Gogli apparatus
Expression	Cell junction; Membrane; Synapse
Structure	Belongs to the small GTPase superfamily, Arf family
Alternative Nomenclature	ADP ribosylation factor 2 antibody ADP ribosylation factor 4 antibody

General Information
Product	Cornichon Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 90-120 on human Cornichon protein.
Accession #	NCBI: AAC98388.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.51-0.62 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	O95406
Overview	Involved in the selective transport and maturation of TGF-alpha family proteins.
Molecular Function	ER-Golgi transport
Subcellular Location	Endoplasmic reticulum membrane; Golgiapparatus membrane
Expression	Highly expressed in heart, liver, skeletal muscle, pancreas, adrenal medulla and cortex, thyroid, testis, spleen, appendix, peripheral blood lymphocytes and bone marrow. Expression is up-regulated in dorsolateral prefrontal cortex of patients with schizophrenia.
Structure	Belongs to cornichon family, contains transmembrane helix
Alternative Nomenclature	CNI like antibody CNIH 2 antibody Cnih3 antibody Cnil antibody RGD1304930 antibody

General Information
Product	DOC2B Antibody
Description	Affinity Purified Double C2-like domain-containing protein beta Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat
Immunogen	Synthetic Peptide taken within amino acid region 360-412 on DOC2b gene.
Accession #	GenBank: AAB47747.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.61 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20°C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Immunohistochemistry	1:50-1:100
Western Blot	1:500
Protein
Uniprot #	P70610
Overview	calcium ion-dependent exocytosis of neurotransmitter;positive regulation of insulin secretion
Molecular Function	calcium-dependent phospholipid binding;transporter activity
Subcellular Location	Cell membrane, Cytoplasm, Membrane
Expression	Expressed in brain; highly enriched in neurons
Alternative Nomenclature	Double C2-like domain-containing protein beta antibody OTTMUSP00000006522 antibody RP23 78H4.2 antibody

General Information
Product	EHD1 Antibody
Description	Affinity Purified EH domain-containing protein 1 Antibody
Verified Applications	ELISA, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken wtihin amino acid region 515-565 on human EH-domain containing protein 1.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.58 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9H4M9
Overview	Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth.
Molecular Function	Rab GTPase binding; ATP binding; calcium ion binding
Subcellular Location	Cell membrane, Endosome, Membrane
Expression	testis
Structure	Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4, ATP-binding is required for heterooligomerization.
Alternative Nomenclature	CDABP0131 antibody EH domain containing 1 antibody FLJ42622 antibody FLJ44618 antibody H PAST antibody PAST antibody PAST1 antibody Testilin antibody

General Information
Product	EHD3 Antibody
Description	Affinity Purified EH domain-containing protein 3 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse Rat
Immunogen	Synthetic peptide taken within amino acid region 475-535 on human EH-domain containing protein 3.
Accession #	NCBI: NP_055416.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.58 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:150
Immunopreciptiation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9NZN3
Overview	Plays a role in endocytic transport
Molecular Function	ATP binding;GTPase activity;nucleic acid binding;calcium ion binding;GTP binding
Subcellular Location	Cell membrane, Cytoplasm, Endosome, Membrane
Expression	Highly expressed in heart and brain and moderately expressed in kidney, liver, and placenta.
Structure	Homooligomer, and heterooligomer with EHD1, EHD2 and EHD4
Alternative Nomenclature	EH domain containing 3 antibody EHD2 antibody EHD3 antibody PAST3 antibody

General Information
Product	EHD4 Antibody
Description	Affinity Purified EH domain-containing protein 4 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse Rat
Immunogen	Synthetic peptide taken within amino acid region 420-450 on EH-domain containing 4 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:100
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9H223
Overview	Plays a role in early endosomal transport
Molecular Function	ATP binding;GTPase activity;nucleic acid binding;calcium ion binding;GTP binding
Subcellular Location	Cell membrane, Endosome, Membrane
Expression	Highly expressed in pancreas and heart.
Structure	Homooligomer, and heterooligomer with EHD1, EHD2 and EHD3
Alternative Nomenclature	EH domain containing 4 antibody Hepatocellular carcinoma associated protein 10/11 antibody HCA11 antibody HGNC:3245 antibody Ortholog of rat pincher antibody PAST4 antibody

General Information
Product	Niemann Pick C1 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 1228-1278 on human Niemann-Pick C1 protein.
Accession #	NCBI: NP_000262.2
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.5 µg/µl in antibody stabilization buffer
Immunoglobulin	IgG
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
ELISA	1:4,000
Immunocytochemistry	1:100
Immunofluorescence	1:100
Immunohistochemistry	1:100
Immunoprecipitation	1:150
Western Blot	1:500
Protein
Uniprot #	O15118
Overview	Intracellular cholesterol transporter plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment.
Molecular Function	Receptor activity
Subcellular Location	Endosome, Lysosome, Membrane
Expression	Adrenal gland; Cerebral cortex; lung; placenta; lymph node
Structure	Belongs to the patched family.
Alternative Nomenclature	Niemann Pick C1 antibody Niemann Pick C1 protein precursor antibody Niemann Pick disease, type C1 antibody NPC antibody NPC1 antibody

General Information
Product	POMT1 Antibody
Description	Affinity Purified Protein O-mannosyl-transferase 1 isoform a Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 400-460 on human POMT1 protein.
Accession #	NCBI: NP_009102.3
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DOT Blot	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:50-1:150
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9Y6A1
Overview	Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.
Molecular Function	mannosyltransferase activity, metal ion binding
Subcellular Location	Endoplasmic reticulum membrane, multi-pass membrane protein
Expression	Widely expressed. Highly expressed in testis, heart and pancreas. Detected at lower levels in kidney, skeletal muscle, brain, placenta, lung and liver.
Alternative Nomenclature	Dolichyl phosphate mannose protein mannosyltransferase 1 antibody LGMD2K antibody POMT 1 antibody Protein O mannosyl transferase 1 antibody

General Information
Product	POMT2 Antibody
Description	Affinity Purified Protein O-mannosyl-transferase 2 Antibody
Verified Applications	ELISA, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey, Mouse, Rat
Immunogen	Synthetic peptide taken within amino acid region 500-560 on human POMT2 protein.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunohistochemistry	1:1000
Immunoprecipitation	1:200
Western Blot	1:500
Protein
Uniprot #	Q9UKY4
Overview	Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient.
Molecular Function	dolichyl-phosphate-mannose-protein mannosyltransferase acitivity, metal ion binding
Subcellular Location	Endoplasmic reticulum membrane, multi-pass membrane protein
Expression	Highly expressed in testis; detected at low levels in most tissues.
Alternative Nomenclature	A830009D15Rik antibody AW046274 antibody DKFZp686G10254 antibody Dolichyl phosphate mannose protein mannosyltransferase 2 antibody FLJ22309 antibody LGMD2N antibody MDDGA2 antibody MDDGB2 antibody MDDGC2 antibody POMT 2 antibody Protein O mannosyltransferase 2 antibody Putative protein O mannosyltransferase antibody rCG_20643 antibody

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ARF1 Antibody

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