General Information

Product

eIF4E3 Antibody 

Description

Affinity Purified Eukaryotic translation initiation factor 4E-3 Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 1-90 on eIF4E3 protein.

Accession #

GenBank:  AAF50460.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9VSG1

Overview

translation initiation factor activity

Molecular Function

Initiation factor

Subcellular Location

cytoplasm

Alternative Nomenclature

eIF-4E type 3 antibody

Eukaryotic translation initiation factor 4E type 3 antibody

General Information

Product

Punt Antibody

Description

Affinity Purified Punt receptor Serine/Threonine kinase (punt) Antibody

Verified Applications

ELISA, ICC, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Synthetic peptide corresponding to C terminal amino acids 497-515 of Drosophila melanogaster PUNT protein. Peptides were amidated for conjugation.

Sequence:

QLLIKNHTNIDDAKESTNC

Accession #

GenBank: L38495.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q24468

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Activin receptor complex, Plasma membrane

Structure

Contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 88CD antibody
Activin receptor type II antibody
Atr antibody
Atr88CD antibody
AtrII antibody
dlhC antibody
Dorsal holes C antibody
j5A5 antibody
Pun antibody
Put antibody
STKC antibody
TGF beta l antibody
TGF beta type receptor like antibody
Tgf r antibody

General Information

Product

Punt Antibody

Description

Affinity Purified Punt receptor Serine/Threonine kinase (punt) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequences on D. melanogaster Punt protein.

Accession #

GenBank: L38495.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q24468

Overview

ATP + [receptor-protein] = ADP + [receptor-protein] phosphate.

Molecular Function

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Subcellular Location

Activin receptor complex, Plasma membrane

Structure

Contains protein kinase domain.

Alternative Nomenclature

Activin A receptor 88CD antibody
Activin receptor type II antibody
Atr antibody
Atr88CD antibody
AtrII antibody
dlhC antibody
Dorsal holes C antibody
j5A5 antibody
Pun antibody
Put antibody
STKC antibody
TGF beta l antibody
TGF beta type receptor like antibody
Tgf r antibody

General Information

Product

SARA Antibody Affinity Purified

Description

Affinity Purified Smad anchor for receptor activation (SARA) mRNA, complete cds Antibody Mid-Region

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 320-370 on SARA protein.

Accession #

GenBank:  AF104305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9YHB9

Molecular Function

Metal ion binding, Receptor

Alternative Nomenclature

CG15667 antibody

LD33044p antibody

Madh-interacting protein antibody

MADHIP antibody

Mothers against decapentaplegic homolog-interacting protein antibody

Novel serine protease antibody

NSP antibody

Receptor activation anchor antibody

SAR1 antibody

SARA1 antibody

smad anchor for Receptor Activation antibody

SMADIP antibody

ZFYVE9 antibody

Zinc finger FYVE domain-containing protein 9 antibody

General Information

Product

SARA Antibody Affinity Purified

Description

Affinity Purified Smad anchor for receptor activation (SARA) mRNA, complete cds Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 1040-1090 on SARA protein.

Accession #

GenBank:  AF104305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9YHB9

Molecular Function

Metal ion binding, Receptor

Alternative Nomenclature

CG15667 antibody

LD33044p antibody

Madh-interacting protein antibody

MADHIP antibody

Mothers against decapentaplegic homolog-interacting protein antibody

Novel serine protease antibody

NSP antibody

Receptor activation anchor antibody

SAR1 antibody

SARA1 antibody

smad anchor for Receptor Activation antibody

SMADIP antibody

ZFYVE9 antibody

Zinc finger FYVE domain-containing protein 9 antibody

General Information

Product

SARA Antibody Affinity Purified

Description

Affinity Purified Smad anchor for receptor activation (SARA) mRNA, complete cds Antibody N-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 260-300 on SARA protein.

Accession #

GenBank:  AF104305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9YHB9

Molecular Function

Metal ion binding, Receptor

Alternative Nomenclature

CG15667 antibody

LD33044p antibody

Madh-interacting protein antibody

MADHIP antibody

Mothers against decapentaplegic homolog-interacting protein antibody

Novel serine protease antibody

NSP antibody

Receptor activation anchor antibody

SAR1 antibody

SARA1 antibody

smad anchor for Receptor Activation antibody

SMADIP antibody

ZFYVE9 antibody

Zinc finger FYVE domain-containing protein 9 antibody

General Information

Product

Shade Antibody Affinity Purified

Description

Affinity Purified Shade, isoform B Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IHC, IF, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 160-230 on Spook protein.

Accession #

NCBI:  NP_996074.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VUF8

Overview

Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxidoreductase

Subcellular Location

Mitochondrion membrane

Expression

Strong expression by embryonic stage 10 in epidermis, decreases significantly in older embryos. Third instar larvae show expression in the midgut copper cells, Malpighian tubules and fat body. In the adult ovaries, expression is seen in both nurse cells a

Structure

Belongs to the cytochrome P450 family.

Alternative Nomenclature

SH2 domain-containing adapter protein D antibody

SHD antibody

SHD_HUMAN antibody

Src homology 2 domain containing transforming protein D antibody Ecdysone 20-monooxygenase

General Information

Product

SHD / Shade Antibody

Description

Affinity Purified Shade, isoform B Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IHC, IF, WB

Host

Rabbit

Species Cross Reactivity

D. melanogaster (fruit fly)

Immunogen

Synthetic peptide taken within amino acid region 1-50 on Ecdysone 20-monooxygenase (SHADE) protein.

Accession #

NCBI:  NP_996074.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VUF8

Overview

Required for CNS development; midline glial cells. Involved in the metabolism of insect hormones; responsible for all ecdysone 20-monooxygenase activity during embryonic, larval and adult stages. May be involved in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxidoreductase

Subcellular Location

Mitochondrion membrane

Expression

Strong expression by embryonic stage 10 in epidermis, decreases significantly in older embryos. Third instar larvae show expression in the midgut copper cells, Malpighian tubules and fat body. In the adult ovaries, expression is seen in both nurse cells a

Structure

Belongs to the cytochrome P450 family.

Alternative Nomenclature

SH2 domain-containing adapter protein D antibody

SHD antibody

Src homology 2 domain containing transforming protein D antibody Ecdysone 20-monooxygenase

General Information

Product

Shadow Antibody

Description

Affinity Purified Shadow Antibody Mid Region

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Synthetic peptide corresponding to unique amino acid sequence near mid region of Shadow protein.

Accession #

NCBI:  NP_650123

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VGH1

Overview

Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxidoreductase, ecdysteroid 2-hydroxylase activity

Subcellular Location

Mitochondrion membrane

Expression

Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and follicle cells of the ovary. In adults, coexpression is seen in the follicle cells, sad only is expressed in nurse cells.

Alternative Nomenclature

CG14728 antibody

Cyp315a1 antibody

CYPCCCXVA1 antibody

Cytochrome P450 315a1 mitochondrial antibody

Karmoisin ghost antibody

LP13573p antibody

sad antibody

Sad PA antibody

shade antibody

General Information

Product

Shadow Antibody 

Description

Affinity Purified Shadow Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide corresponding to unique amino acid sequence near C-terminal of Shadow protein.

Accession #

NCBI:  NP_650123

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9VGH1

Overview

Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.

Molecular Function

Monooxygenase, Oxidoreductase, ecdysteroid 2-hydroxylase activity

Subcellular Location

Mitochondrion membrane

Expression

Complex coexpression pattern of dib (disembodied) and sad (shade) in the early embryo that restricts to the prothoracic gland cells of the developing ring gland during late embryogenesis. In larvae and adult, coexpression is seen in prothoracic gland and follicle cells of the ovary. In adults, coexpression is seen in the follicle cells, sad only is expressed in nurse cells.

Alternative Nomenclature

CG14728 antibody

Cyp315a1 antibody

CYPCCCXVA1 antibody

Cytochrome P450 315a1 mitochondrial antibody

Karmoisin ghost antibody

LP13573p antibody

sad antibody

Sad PA antibody

shade antibody