General Information

Product

MC4 Receptor Antibody

Description

Affinity Purified Melanocortin receptor 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 10-60 on human MC4R protein.

Accession #

NCBI:  NP_005903.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P32245

Overview

Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP).

Molecular Function

Melanocortin receptor activity, melanocyte-stimulating hormone receptor activity, neuropeptide binding, ubiquitin protein ligase binding

Subcellular Location

cell membrane, multi-pass membrane protein, membrane raft. (Note: Localizes to membrane rafts but is also loated in the cell membrane outside of these regions where channel response to cold is enhanced compared to membrane rafts).

Expression

Brain, placental, and gut tissues.

Alternative Nomenclature

MC4 R antibody

Melanocortin receptor 4 antibody

MGC126851 antibody

MGC138197 antibody

General Information

Product

ACTH Antibody

Description

Affinity Purified Adrenocorticotropin Hormone Antibody

Verified Applications

ELISA, IHC-Fr, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Predicted: Chicken, Chimpanzee, Cow, Dog, Goat, Guinea pig, Macaque Monkey, Orangutan, Sheep

Immunogen

Synthetic peptide corresponding to ACTH aa 138-176. 

Sequence:

SYSMEHFRWGKPVGKKRRPVKVYPNGAEDESAEAFPLEF

Accession #

NCBI:  AAX36901

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.67-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Immunocytochemistry

1:250

Immunofluorescence

1:200

Immunohistochemistry

1:250

Immunoprecipitation

1:200

Western Blot

1:1000 Predicted Molecular weight: 29 kDa

Protein

Uniprot #

UPI00004EBE53

Overview

Stimulates adrenal glands and release cortisol.

Molecular Function

GPCR; Hormone activity

Subcellular Location

Secreted

Expression

ACTH and MSH are produced by the pituitary gland

Structure

Belongs to POMC family

Alternative Nomenclature

Alpha Melanocyte Stimulating Hormone antibody

Beta Endorphin antibody

Beta Lipotropin antibody

Beta Melanocyte Stimulating Hormone antibody

Beta-endorphin antibody

CLIP antibody

Corticotropin Like Intermediary Peptide antibody

Met-enkephalin antibody

MSH antibody

NPP antibody

Opiomelanocortin prepropeptide antibody

POC antibody

POMC antibody

Tetracosactide antibody

General Information

Product

Estrogen Receptor alpha Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide taken within amino acid region 30-80 on human ESTRA protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P03372

Overview

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues

Molecular Function

Activator, Receptor

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

Widely expressed. Isoform 3 is not expressed in the pituitary gland

Structure

Belongs to the nuclear hormone receptor family. NR3 subfamily

Alternative Nomenclature

DKFZp686N23123 antibody

ER alpha antibody

ER antibody

Era antibody

ESR antibody

ESR1 antibody

ESRA antibody

Estradiol receptor antibody

Estrogen nuclear receptor alpha antibody

Estrogen receptor 1 antibody

Estrogen receptor antibody

ESTRR antibody

Major ORF antibody

NR3A1 antibody

Nuclear receptor subfamily 3 group A member 1 antibody

General Information

Product

FSH Antibody

Description

Affinity Purified Follicle stimulating hormone Antibody

Verified Applications

CM, ELISA, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-60 on human FSH protein.

Accession #

GenBank: ABD84005

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.71 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

Dot Blot

1:50,000

ELISA

1:50,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:500

Protein

Uniprot #

Q1W658

Overview

Binds to activin directly and serves as an activin antagonist.  Acts as a specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone.

Subcellular Location

Secreted

Structure

Monomer

Alternative Nomenclature

Follicle stimulating hormone receptor isoform 1 antibody

Follitropin receptor antibody

FSH receptor antibody

FSH-R antibody

FSHRO antibody

LGR1 antibody

MGC141667 antibody

MGC141668 antibody

ODG1 antibody

ovarian dysgenesis 1 antibody

General Information

Product

Norepinephrine Transporter Antibody

Description

Affinity Purified Sodium -dependent noradrenaline transporter Antibody

Verified Applications

Dot Blot, ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to N-terminal amino acids that are unique to noradrenaline transporter protein. The antibodies are raised utilizing linear, multiple antigenic peptides or cyclic peptide methodology.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.61 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage Instructions

Shipped at 4⁰C. Upon delivery aliquot and store at -20⁰C or -80⁰C. Avoid repeated freeze / thaw cycles.

pH: 7.80
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris glycine, 30% Glycerol

Application Protocol

Confocal Microscopy

1:100

DB

1:4,000

ELISA

1:4,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

P23975

Overview

Amine transporter. Terminates the action of noradrenaline by its high affinity sodium-dependent reuptake into presynaptic terminals.

Molecular Function

monoamine transmembrane transporter activity; norepinephrine:sodium symporter activity

Subcellular Location

Membrane

Expression

Adrenal gland; Placenta; Testis; Skin

Structure

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A2 subfamily.

Alternative Nomenclature

NAT1 antibody

NET antibody

NET1 antibody

Neurotransmitter transporter antibody

Norepinephrine transporter antibody

SLC6A2 antibody

SLC6A5 antibody

Sodium dependent noradrenaline transporter antibody

Solute carrier family 6 (neurotransmitter transporter norepinephrine) member 5 antibody

Solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 antibody

Solute carrier family 6 member 2 antibody

Solute carrier family 6 member 5 antibody

General Information

Product

TMEPAI Antibody

Description

Affinity Purified Prostate transmembrane protein, androgen induced 1 Antibody

Verified Applications

Dot Blot, ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Purified TMEPAI protein which was emulsified with adjuvants to acheive the desired antigenicity.

Accession #

NCBI:  NP_035719.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

.65-.694 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

D2KUZ7

Overview

May function as a negative regulator of TGF-beta signaling and thereby inhibits activin-mediated mesoderm formation.

Molecular Function

signal transduction inhibitor

Subcellular Location

Early endosome membrane; Single-pass membrane protein, Golgi apparatus membrane; Single-pass membrane protein

Alternative Nomenclature

PMEPA1 antibody

Prostate transmembrane protein, androgen induced 1 antibody

Solid tumor associated 1 protein antibody

STAG1 antibody

TMEPAI antibody

Transmembrane prostate androgen induced protein antibody

General Information

Product

Progesterone Receptor Antibody

Description

Affinity Purified Progesterone Receptor Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 350-400 on human Progesterone receptor protein.

Accession #

GenBank: AAD01587.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06401

Overview

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Molecular Function

DNA binding; Enzyme binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; Steriod hormone receptor activity; Transcription regulation

Subcellular Location

Nucleus. Cytoplasm
Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases.
Isoform A : Nucleus. Cytoplasm
Note: Mainly nuclear.
Isoform 4 : Mitochondrion outer membrane
mitochondrial outer membrane
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Structure

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2.

Alternative Nomenclature

NR3C3 antibody

Nuclear receptor subfamily 3 group C member 3 antibody

PGR antibody

PR antibody

PR antibody

PRA antibody

PRB antibody

Progesterone receptor antibody

Progestin receptor form A antibody

Progestin receptor form B antibody

Progestin receptor form C antibody

General Information

Product

Progesterone Receptor Antibody

Description

Affinity Purified Progesterone Receptor Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 320-370 on human Progesterone receptor protein.

Accession #

GenBank: AAD01587.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P06401

Overview

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation.
Isoform A: inactive in stimulating c-Src/MAPK signaling on hormone stimulation.
Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.

Molecular Function

DNA binding; Enzyme binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; Steriod hormone receptor activity; Transcription regulation

Subcellular Location

Nucleus. Cytoplasm
Note: Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G1 and G2/M phases.
Isoform A : Nucleus. Cytoplasm
Note: Mainly nuclear.
Isoform 4 : Mitochondrion outer membrane
mitochondrial outer membrane
Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Structure

Interacts with SMARD1 and UNC45A. Interacts with CUEDC2; the interaction promotes ubiquitination, decreases sumoylation, and repesses transcriptional activity. Interacts with PIAS3; the interaction promotes sumoylation of PR in a hormone-dependent manner, inhibits DNA-binding, and alters nuclear export. Interacts with SP1; the interaction requires ligand-induced phosphorylation on Ser-345 by ERK1/2 MAPK. Interacts with PRMT2.

Alternative Nomenclature

NR3C3 antibody

Nuclear receptor subfamily 3 group C member 3 antibody

PGR antibody

PR antibody

PR antibody

PRA antibody

PRB antibody

Progesterone receptor antibody

Progestin receptor form A antibody

Progestin receptor form B antibody

Progestin receptor form C antibody

General Information

Product

TSH beta Antibody Affinity Purified

Description

Affinity Purified Thyroid stimulating hormone, beta Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human TSH beta chain protein.

Accession #

GenBank: AAH69298.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.63-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:50,000

ELISA

1:50,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Western Blot

1:500

Protein

Uniprot #

P01222

Overview

Indespensable for the control of thyroid structure and metabolism.

Molecular Function

hormone activity

Subcellular Location

Secreted

Alternative Nomenclature

CHNG4 antibody

Thyroid stimulating hormone beta subunit antibody

Thyrotropin beta subunit antibody

TSHB antibody