General Information
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Product
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ergo-1 Antibody BIOTIN
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Description
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BIOTIN-Conjugated Endogenous-RNAi deficient arGOnaute protein N-epitope
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Caenorhabditis elegans
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Immunogen
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Synthetic peptide taken within amino acid region 790-840 on C.elegans ergo-1 protein.
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Accession #
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NCBI: NP_503362.2
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.62-0.68 µg/µl in antibody stabilization buffer
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Form
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BIOTIN-Conjugated
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Clonality
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Polyclonal
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Determinant
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N-epitope
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Storage
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-20⁰C for long term storage
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Application Protocol
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DB
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1:10,000
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ELISA
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1:10,000
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Immunoprecipitation
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1:150
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Western Blot
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1:500
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Protein
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Uniprot #
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O61931
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Overview
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Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway. Interacts with 26G small interfering RNAs (26G-siRNAs) and required for subsequent biogenesis of secondary 22G-siRNAs. May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed: 22102828).
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Molecular Function
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Dead/H-box RNA helicase binding, nucleic acid binding, production of siRNA involved in RNA interference, regulation of translation, RNA interference.
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Subcellular Location
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Cytoplasm
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Structure
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Belongs to the argonaute family. Piwi subfamily. Contains 1 PAZ domain. Contains 1 Piwi domain.
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Expression |
Expressed in embryos and during larval development up to larval stage 2. |
Alternative Nomenclature
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Endogenous RNAi deficient argonaute antibody
Ergo 1 antibody
Hypothetical protein R09A1.1 antibody
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