General Information

Product

Eprhin Receptor A5 Antibody Affinity Purified

Description

Affinity Purified Ephrin Type-1 Receptor isoform a Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 600-700 on human EphA R5 protein.

Accession #

NCBI:  NP_004430

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P54756

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

Alternative Nomenclature

Brain-specific kinase antibody

bsk antibody

CEK 7 antibody

Eck like sequence 1 antibody

EHK 1 antibody

EK7 antibody

Els 1 antibody

Eph homology kinase 1 antibody

EPH-like kinase 7 antibody

EphA5 antibody

Ephrin type-A receptor 5 antibody

HEK 7 antibody

Rek 7 antibody

TYRO 4 antibody

Tyrosine protein kinase receptor EHK 1 antibody

General Information

Product

Eprhin Receptor B1 Antibody

Description

Affinity Purified Ephrin type-B receptor 1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP ,WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 850-900 on mouse Eph BR1 protein.

Accession #

NCBI:  NP_775623

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

Q8CBF3

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Dendrite

Expression

Expressed in neural stem and progenitor cells in the dentate gyrus.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1. Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion. Then ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1.

Alternative Nomenclature

Cek 6 antibody

EK6 antibody

ELK antibody

Elkh antibody

Eph tyrosine kinase 2 antibody

EPH-like kinase 6 antibody

EPH2 antibody

EphB1 antibody

Ephrin receptor Eph B1 precursor antibody

Ephrin type B receptor 1 antibody

EPHT2 antibody

HEK 6 antibody

NET antibody

Neuronally expressed EPH related tyrosine kinase antibody

Tyrosine-protein kinase receptor EPH-2 antibody

General Information

Product

Eprhin Receptor B2 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-B receptor 2 isoform 2 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-150 on mouse Ephrin BR2 protein.

Accession #

NCBI:  NP_034272

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

A3KG01

Overview

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane

Structure

Contains sterile alpha motif domain; Conains protein kinase domain

Alternative Nomenclature

Cek 6 antibody

EK6 antibody

ELK antibody

Elkh antibody

Eph tyrosine kinase 2 antibody

EPH-like kinase 6 antibody

EPH2 antibody

EphB1 antibody

Ephrin receptor Eph B1 precursor antibody

Ephrin type B receptor 1 antibody

EPHT2 antibody

HEK 6 antibody

NET antibody

Neuronally expressed EPH related tyrosine kinase antibody

Tyrosine-protein kinase receptor EPH-2 antibody

General Information

Product

FGFR2 Antibody

Description

Affinity Purified Fibroblast Growth Factor Receptor 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 520-570 on human Fibroblast Growth Factor receptor 2 isoform X6 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P21802

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.

Molecular Function

ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle

Note: Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded.

Isoform 1 : Cell membrane

Expression

Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle.

Structure

Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF6, FGF7, FGF8, FGF9, FGF10, FGF17, FGF18 and FGF22 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Isoform 1 has high affinity for FGF1 and FGF2, but low affinity for FGF7. Isoform 3 has high affinity for FGF1 and FGF7, and has much higher affinity for FGF7 than isoform 1 (in vitro). Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19 and FGF21. Interacts with PLCG1, GRB2 and PAK4.

Alternative Nomenclature

Bacteria expressed kinase antibody

BBDS antibody

BEK antibody

BEK fibroblast growth factor receptor antibody

BFR1 antibody

CD 332 antibody

CEK 3 antibody

CFD1 antibody

Craniofacial dysostosis 1 antibody

Crouzon syndrome antibody

ECT 1 antibody

FGFR 2 antibody

Fibroblast growth factor receptor 2 antibody

Hydroxyaryl protein kinase antibody

Jackson Weiss syndrome antibody

JWS antibody

K SAM antibody

Keratinocyte growth factor receptor 2 antibody

KGFR antibody

KSAM antibody

Pfeiffer syndrome antibody

Protein tyrosine kinase receptor like 14 antibody

soluble FGFR4 variant 4 antibody

TK14 antibody

TK25 antibody

Tyrosylprotein kinase antibody

General Information

Product

Phospho-FGFR2 Antibody

Description

Affinity Purified Phospho-Fibroblast Growth Factor Receptor 2 Antibody (Y769)

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human

Immunogen

Synthetic peptide taken within amino acid region 520-570 on human Fibroblast Growth Factor receptor 2 protein.

Accession #

NCBI:  XP_011537812.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P21802

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. 

Molecular Function

ATP-binding; Fibroblast growth factor binding; Heparin binding; Protein Tyrosine Kinase activity

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle

Note: Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded.

Isoform 1 : Cell membrane

Expression

Expressed in brain, kidney and testis. Very low or no expression in spleen, heart, and muscle. In 20- to 22-week old fetuses it is expressed at high level in kidney, lung, small intestine and brain, and to a lower degree in spleen, liver, and muscle.

Structure

Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF6, FGF7, FGF8, FGF9, FGF10, FGF17, FGF18 and FGF22 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Isoform 1 has high affinity for FGF1 and FGF2, but low affinity for FGF7. Isoform 3 has high affinity for FGF1 and FGF7, and has much higher affinity for FGF7 than isoform 1 (in vitro). Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19 and FGF21. Interacts with PLCG1, GRB2 and PAK4.

Alternative Nomenclature

Bacteria expressed kinase antibody

BBDS antibody

BEK antibody

BEK fibroblast growth factor receptor antibody

BFR1 antibody

CD 332 antibody

CEK 3 antibody

CFD1 antibody

Craniofacial dysostosis 1 antibody

Crouzon syndrome antibody

ECT 1 antibody

FGFR 2 antibody

Fibroblast growth factor receptor 2 antibody

Hydroxyaryl protein kinase antibody

Jackson Weiss syndrome antibody

JWS antibody

K SAM antibody

Keratinocyte growth factor receptor 2 antibody

KGFR antibody

KSAM antibody

Pfeiffer syndrome antibody

Protein tyrosine kinase receptor like 14 antibody

soluble FGFR4 variant 4 antibody

TK14 antibody

TK25 antibody

Tyrosylprotein kinase antibody

General Information

Product

Lyn Antibody

Description

Affinity Purified Tyrosine-protein kinase Lyn Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Immunogen

Synthetic peptide taken within amino acid region 425-525 on human Lyn protein.

Accession #

NCBI:  NP_002341

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P07948

Overview

Non-receptor tyrosine-protein kinase that plays role in regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

Expression

monocytes,placenta,fetal brain, lung, liver,kidney and epidermoid, hematopoietic, and neuronal cells,also primary neuroblastoma tumors

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily

Alternative Nomenclature

AA407514 antibody

EC 2.7.10.2 antibody

FLJ26625 antibody

Hck 2 antibody

JTK 8 antibody

Lck/Yes related novel protein tyrosine kinase antibody

LYN antibody

ONCOGENE LYN antibody

p53Lyn antibody

p56Lyn antibody

Tyrosine protein kinase LYN antibody

V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 130-180 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 and 950-976 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit Antibody Mid-Region

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 430-480 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody