General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody Multi-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit fly, Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptides taken within amino acid region 400-450 and 502-552 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1. Interacts with CITED2. Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody Mid-Region Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Fruit fly, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 400-450 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region Epitope

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 502-552 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Smad4 Antibody

Description

Affinity Purified Mothers against decapentaplegic homolog 4 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 131-181 on human SMAD4 protein.

Accession #

NCBI:  NP_005350.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13485

Overview

In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.

Molecular Function

Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding

Subcellular Location

Cytoplasm, nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.

Structure

Found in a complex with SMAD1 and YY1. Interacts with CITED2. Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody DPC4 antibody
JIP antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody

MADH4 antibody
Med antibody
Medea antibody

Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody

MYHRS antibody
SMA- and MAD-related protein 4 antibody
Smad 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody

General Information

Product

Visfatin Antibody

Description

Affinity Purified Nicotinamide phosphoribosyltransferase precursor Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

N-epitope synthetic peptide taken within amino acid region 200-300 on human Visfatin protein.

Accession #

NCBI:  NP_005737.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P43490

Overview

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma.

Molecular Function

Cytokine, Glycosyltransferase, Transferase

Subcellular Location

Nucleus, Cytoplasm, Secreted

Expression

Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Structure

Homodimer

Alternative Nomenclature

1110035O14Rik antibody

AI314458 antibody

AI480535 antibody

DKFZP666B131 antibody

EC 2.4.2.12 antibody

MGC117256 antibody

NAmPRTase antibody

Nampt antibody

Nicotinamide phosphoribosyltransferase antibody

PBEF antibody

PBEF1 antibody

Pre B cell colony enhancing factor antibody

Pre B cell enhancing factor antibody

VF antibody

Visfatin antibody

General Information

Product

Visfatin Antibody

Description

Affinity Purified Nicotinamide phosphoribosyltransferase precursor Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

C-epitope synthetic peptide taken within amino acid region 400-490 on human Visfatin protein.

Accession #

NCBI:  NP_005737.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P43490

Overview

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma.

Molecular Function

Cytokine, Glycosyltransferase, Transferase

Subcellular Location

Nucleus, Cytoplasm, Secreted

Expression

Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Structure

Homodimer

Alternative Nomenclature

1110035O14Rik antibody

AI314458 antibody

AI480535 antibody

DKFZP666B131 antibody

EC 2.4.2.12 antibody

MGC117256 antibody

NAmPRTase antibody

Nampt antibody

Nicotinamide phosphoribosyltransferase antibody

PBEF antibody

PBEF1 antibody

Pre B cell colony enhancing factor antibody

Pre B cell enhancing factor antibody

VF antibody

Visfatin antibody

General Information

Product

iNOS Antibody

Description

Affinity Purified Nitric oxide synthase, inducible Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1064-1114 on human inducible nitric oxide synthase protein.

Specificity

This antibody will detect both iNOS and iNOS2 protein.

Accession #

GenBank: BAA37123.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P35228

Overview

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Molecular Function

Arginine binding, FMN binding, iron ion binding, NADPH-hemoprotein reductase activity, nitric-oxide synthase activity, receptor binding, heme binding, NADP binding

Subcellular Location

cortical cytoskeleton; cytoplasm; cytosol; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome

Expression

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Structure

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Alternative Nomenclature

HEP NOS antibody

Hepatocyte NOS antibody

HEPNOS antibody

inducible antibody

Inducible nitric oxide synthase antibody

Inducible NO synthase antibody

Inducible NOS antibody

INOS antibody

MAC NOS antibody

Macrophage NOS antibody

Nitric oxide synthase 2 inducible macrophage antibody

nitric oxide synthase 2A (inducible, hepatocytes) antibody

Nitric oxide synthase antibody

Nitric oxide synthase inducible antibody

nitric oxide synthase, macrophage antibody

NOS 2 antibody

NOS antibody

NOS2A antibody

NOS2A, Inducible, Hepatocyte antibody

Peptidyl-cysteine S-nitrosylase NOS2 antibody

General Information

Product

iNOS Antibody

Description

Affinity Purified Nitric oxide synthase, inducible Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human inducible nitric oxide synthase protein.

Specificity

This antibody will detect both iNOS and iNOS2 protein.

Accession #

GenBank: BAA37123.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P35228

Overview

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Molecular Function

Arginine binding, FMN binding, iron ion binding, NADPH-hemoprotein reductase activity, nitric-oxide synthase activity, receptor binding, heme binding, NADP binding

Subcellular Location

cortical cytoskeleton; cytoplasm; cytosol; intracellular; nucleus; perinuclear region of cytoplasm; peroxisome

Expression

Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

Structure

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Alternative Nomenclature

HEP NOS 

Hepatocyte NOS 

Inducible nitric oxide synthase

Inducible NO synthase 

INOS

MAC NOS 

Macrophage NOS 

Nitric oxide synthase 2 inducible macrophage 

Nitric oxide synthase 

NOS 2 

NOS 

NOS2A 

NOS2A, Inducible, Hepatocyte 

Peptidyl-cysteine S-nitrosylase NOS2 

General Information

Product

TTC27 Antibody Affinity Purified

Description

Affinity Purified tetratricopeptide repeat protein 27 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat, Zebra Fish

Immunogen

Synthetic peptide taken within amino acid region 358-408 on tetratricopeptide repeat protein 27 Danio rerio protein.

Accession #

NCBI:  NP_775392.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8CD92

Structure

Belongs to the TTC27 family.  Contains 6 TPR repeats.

Alternative Nomenclature

FLJ20272 antibody

Tetratricopeptide repeat domain 27 antibody

TPR repeat protein 27 antibody

TTC27 antibody

General Information

Product

FAK2 Antibody

Description

Affinity Purified Protein-tyrosine kinase 2-beta / Focal adhesion Kinase 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 700-750 on human focal adhesion kinase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q14289

Overview

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca2+ levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.

Molecular Function

Kinase, Transferase, Tyrosine-protein Kinase

Subcellular Location

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Expression

Most abundant in the brain, with highest levels in amygdala and hippocampus. Low levels in kidney (at protein level). Also expressed in spleen and lymphocytes.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.

Contains 1 FERM domain.

Contains 1 protein kinase domain.

Alternative Nomenclature

CADTK antibody

CAK beta antibody

CAKB antibody

Calcium dependent tyrosine kinase antibody

Calcium regulated non receptor proline rich tyrosine kinase antibody

Cell adhesion kinase beta antibody

E430023O05Rik antibody

EC 2.7.10.2 antibody

FADK2 antibody

FAK2 antibody

Focal Adhesion Kinase 2 antibody

MGC124628 antibody

PKB antibody

pp125FAK antibody

Proline Rich Tyrosine Kinase 2 antibody

Protein kinase B antibody

Protein Tyrosine Kinase 2 Beta antibody

PTK antibody

PTK2B protein tyrosine kinase 2 beta antibody

PYK 2 antibody

RAFTK antibody

RAFTK2 antibody

Related adhesion focal tyrosine kinase antibody