General Information
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Product
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VPS36 Antibody
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Verified Applications
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ELISA, IP, WB
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Host
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Rabbit
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Species Cross Reactivity
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Human
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Immunogen
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Synthetic peptide taken within amino acid region 250-310 on human Vacuolar Protein-Sorting-associated protein 36 protein.
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Accession #
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NCBI: NP_057159
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Physical Properties
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Quantity
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100 µg
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Volume
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200 µl
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Concentration
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0.5-1.5 µg/µl in antibody stabilization buffer
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Immunoglobulin
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IgG
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Form
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Affinity Purified
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Purification Method
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Immobilized antigen affinity chromatography
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Clonality
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Polyclonal
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Storage
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-20⁰C for long term storage
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Application Protocol
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ELISA
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1:50,000
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Immunoprecipitation
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1:200
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Western Blot
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1:2,500-1:5,000
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Protein
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Uniprot #
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Q86VN1
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Overview
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Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.
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Molecular Function
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phosphatidylinositol-3-phosphate binding
protein C-terminus binding
ubiquitin binding
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Subcellular Location
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Nucleus
Endosome
Late endosome
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Expression
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Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45. Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (PubMed:14519844). Interacts with VPS25, SNF8, TSG101 and CHMP6 (PubMed:14505570, PubMed:14519844, PubMed:16973552, PubMed:18539118). Interacts (via GLUE domain) with ubiquitin (PubMed:17057716). Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (PubMed:17010938). Interacts with ECPAS (PubMed:20682791).
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Structure
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The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.
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Alternative Nomenclature
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C13orf9 antibody
CGI 145 antibody
EAP45 antibody
ELL associated protein of 45 kDa antibody
ESCRT-II complex subunit VPS36 antibody
Vacuolar protein-sorting-associated protein 36 antibody
Vps36 antibody
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