General Information

Product

DEK Antibody

Description

Affinity Purified DEK Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Predicted: Cat, Cow

Immunogen

Synthetic peptide taken within residues corresponding to a region within amino acids 324-373 of human DEK.

Region:

ELKETIKKLL ASANLEEVTM KQICKKVYEN YPTYDLTERK DFIKTTVKEL

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

Dot blot

1:10,000

ELISA

1:10,000

Western Blot

1:500-1:750

Predicted molecular weight: 43 kDa

Protein

Uniprot #

P35659

Overview

Involved in chromatin organization

Molecular Function

Chromatin regulator; DNA-binding

Subcellular Location

Nucleus

Expression

Ubiquitous

Structure

Contains 1 SAP domain

Alternative Nomenclature

D6S231E antibody

General Information

Product

AIM2 Antibody

Description

Affinity Purified Interferon-inducible protein AIM2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 240-340 on human AIM2 protein.

Accession #

NCBI:  NP_004824

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O14862

Overview

Innate immune response recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages.  Tumor suppressor which may act by repressing NF-kappa-B transcriptional activity.

Molecular Function

Apoptosis, Immunity, Inflammatory response, Innate Immunity

Expression

Expressed in spleen, small intestine, peripheral blood leukocytes and testis. 

Structure

Self-associates; forms homodimers in response to cytosolic dsDNA and the dsDNA serves as oligomerization platform.

Alternative Nomenclature

Absent in melanoma 2 antibody

PYHIN4 antibody

General Information

Product

Phospho Smad1 Antibody

Description

Affinity Purified Protein mothers against dpp Antibody

Verified Applications

ELISA, ICC/IF, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 405-455 on D. melanogaster Mothers against dpp protein.

Accession #

NCBI:  NP_477017.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.0 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P42003

Overview

Required for the function of decapentaplegic. May play an important role in mediating Dpp signaling. Involved in the BMP signaling pathway.

Molecular Function

DNA binding; RNA polymerase II activating transcription factor; Transcription regulatory region sequence-specific activity; Transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity

Subcellular Location

Cytoplasm; Nucleus

Expression

Is detected in all developmental stages, though it appears most abundant in pupae, adult stages and early embryos. Its abundance decreases throughout embryonic and larval development and then returns to high levels in pupae and adult females.

Structure

Homotrimer

Alternative Nomenclature

BSP 1 antibody

Dwarfin A antibody

DwfA antibody

JV 41 antibody

MAD homolog 1 antibody

MAD mothers against decapentaplegic homolog 1 antibody

Mad related protein 1 antibody

MADR1 antibody

Mothers against DPP homolog 1 antibody

Sma and Mad related protein 1 antibody

SMAD 1 antibody

Spinal muscular atrophy distal with upper limb predominance antibody

TGF beta signaling protein 1 antibody

Transforming growth factor beta signaling protein 1 antibody

General Information

Product

Topoisomerase II alpha Antibody

Description

Affinity Purified DNA topoisomerase (ATP-hydrolysing) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

Accession #

GenBank: CAA09762.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P11388

Overview

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Molecular Function

Isomerase, Topoisomerase, ATP binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity.

Alternative Nomenclature

alpha isozyme antibody

ATP hydrolyzing DNA topoisomerase II alfa antibody

DNA gyrase antibody

DNA topoisomerase 2 alpha antibody

DNA topoisomerase II 170 kD antibody

DNA topoisomerase II alpha isozyme antibody

TOP2 antibody

TOP2A antibody

Topoisomerase DNA II alpha 170kDa antibody

TP2A antibody

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

General Information

Product

C1orf146 Antibody

Description

Affinity Purified Chromosome 1 open reading frame 146 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-180 on human C1orf146 protein.

Accession #

NCBI:  NP_001012425

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q5VVC0

Overview

Uncharacterized protein C1orf146

Alternative Nomenclature

C1orf146 antibody

CA146_HUMAN antibody

Chromosome 1 open reading frame 146 antibody

RP11-163M2.4 antibody

Uncharacterized protein C1orf146 antibody

General Information

Product

NT5C2 Antibody

Description

Affinity Purified 5'-nucleotidase, cytosolic II Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique aino acid sequence on human cNII protein.

Accession #

GenBank: CAI40080

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P49902

Overview

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.

Molecular Function

Magnesium, Metal-binding, Nucleotide-binding

Subcellular Location

Cytoplasm

Structure

Homotetramer

Alternative Nomenclature

5' nucleotidase (purine) cytosolic type B antibody

cN II antibody

Cytosolic 5''-nucleotidase II antibody

Cytosolic purine 5''-nucleotidase antibody

GMP antibody

NT5B antibody

nt5c2 antibody

NT5CP antibody

NUCLEOTIDASE, 5-PRIME, CYTOSOLIC II antibody

PNT5 antibody

PURINE 5-PRIME-NUCLEOTIDASE antibody

General Information

Product

DNA polymerase eta Antibody

Description

Affinity Purified Chain B, Yeast DNA polymerase Eta Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Saccharomyces cervisiae

Immunogen

Synthetic peptide taken within amino acid region 200-300 on Chain A, Yeast DNA polymerase Eta.

Accession #

NCBI:  1JIH_A

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.70-0.98 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q04049

Overview

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Plays an important role in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. Efficiently incorporates nucleotides opposite to other UV or oxidative DNA damages like O(6)-methylguanine, 7,8-dihydro-8-oxoguanine, 2,6-diamino-4-hydroxy-5-formamidopyrimidine of 2'-deoxyguanosine (FaPydG), or p-benzoquinone DNA adducts.

Molecular Function

Damaged DNA binding, Metal ion binding, DNA-directed DNA polymerase activity

Subcellular Location

Nucleus

Expression

Induced by UV radiation and heat shock.  The mRNA is stabilized during stationary phase.

Structure

Interacts with POL30. This interaction is essential for the polymerase eta function.

Alternative Nomenclature

DNA polymerase eta antibody

FLJ16395 antibody

FLJ21978 antibody

POLH antibody

polymerase DNA directed eta antibody

RAD30 antibody

RAD30 homolog A antibody

Xeroderma pigmentosum variant type protein antibody

XPV antibody

General Information

Product

CMT Antibody

Description

Affinity Purified Protein-S-isoprenylcysteine O-methyltransferase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 230-284 on human Protein-S-isoprenylcysteine O-methyltransferase.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O60725

Overview

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.

Molecular Function

Methyltransferase

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein

Expression

Ubiquitous

Structure

Belongs to class VI-like SAM-bidning methyltransferase superfamily.  Isoprenylcysteine carboxyl methyltransferase family

Alternative Nomenclature

fcmt 

HSTE14 

Icmt 

Isoprenylcysteine carboxylmethyltransferase 

MST098 

pcCMT 

Prenylcysteine carboxyl methyltransferase 

Protein-S-isoprenylcysteine O-methyltransferase 

STE14 

General Information

Product

PALB2 Antibody

Description

Affinity Purified Partner and localizer of BRCA2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1,130-1,186 on human PALB2 protein.

Accession #

GenBank: AAH44254.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q86YC2

Molecular Function

DNA binding

Subcellular Location

Nucleus

Structure

Homooliomer; dissociated once DNA damage has occurred which allows association with BRCA1.

Alternative Nomenclature

4732427B05 antibody

BC066140 antibody

DKFZp667I166 antibody

DKFZp686E1054 antibody

FANCN antibody

FLJ21816 antibody

OTTMUSP00000025884 antibody

PALB2 antibody

Partner and localizer of BRCA2 antibody

PNCA3 antibody

RGD1304759 antibody

General Information

Product

Phospho NT5C2 Antibody Affinity Purified

Description

Affinity Purified Phospho 5'-nucleotidase, cytosolic II Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on cNII protein with phosphorylated Serine 7.

Accession #

GenBank: CAI40080

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.85 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P49902

Overview

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.

Molecular Function

Magnesium, Metal-binding, Nucleotide-binding

Subcellular Location

Cytoplasm

Structure

Homotetramer

Alternative Nomenclature

5' nucleotidase (purine) cytosolic type B antibody

cN II antibody

Cytosolic 5''-nucleotidase II antibody

Cytosolic IMP/GMP-specific 5'-nucleotidase antibody

GMP antibody

NT5B antibody

NT5CP antibody

NUCLEOTIDASE, 5-PRIME, TYPE B antibody

PNT5 antibody

PURINE 5-PRIME-NUCLEOTIDASE antibody