General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 915-965 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

cKit Antibody

Description

Affinity Purified Tyrosine-protein kinase Kit

Antibody Extreme C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Dog, Human, Monkey, Pig

Immunogen

Synthetic peptide taken within amino acid region 950-976 on human Tyrosine-Kinase c-Kit protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P10721

Overview

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation.

Molecular Function

ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity

Subcellular Location

Isoform 1 : Cell membrane; Single-pass type I membrane protein

Isoform 2 : Cell membrane; Single-pass type I membrane protein

Isoform 3 : Cytoplasm

Expression

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Structure

Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC.

Alternative Nomenclature

c-Kit antibody

CD117 antigen antibody

KIT antibody

KIT oncogene antibody

Mast cell growth factor receptor antibody

p145 c-kit antibody

PBT antibody

Piebald trait protein antibody

Proto oncogene c Kit antibody

SCF Receptor antibody

SCFR antibody

soluble KIT variant 1 antibody

Steel Factor Receptor antibody

Stem cell factor receptor antibody

Tyrosine-protein kinase Kit antibody

v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody

General Information

Product

FXIIIA Antibody

Description

Affinity Purified Blood coagulation factor XIII a subunit Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human factor XIII A subunit protein.

Accession #

GenBank: AAA52415.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P00488

Overview

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

Molecular Function

Metal ion binding; Protein-glutamine gamma-glutamyltransferase activity

Subcellular Location

Cytoplasm. Secreted

Structure

Tetramer of two A chains and two B chains.

Alternative Nomenclature

bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody

Coagulation factor XIII A chain antibody

F13A antibody

F13A1 antibody

Fibrin stabilizing factor, A subunit antibody

Fibrinoligase antibody

FSF, A subunit antibody

FXIIIA antibody

Protein-glutamine gamma-glutamyltransferase A chain antibody

TGase antibody

Transglutaminase A chain antibody

General Information

Product

FXIIIA Antibody

Description

Affinity Purified Blood coagulation factor XIII a subunit Antibody C-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Cat, Human, Monkey, Mouse

Immunogen

Synthetic peptide taken within amino acid region 700-732 on human factor XIII A subunit protein.

Accession #

GenBank: AAA52415.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P00488

Overview

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

Molecular Function

Metal ion binding; Protein-glutamine gamma-glutamyltransferase activity

Subcellular Location

Cytoplasm. Secreted

Structure

Tetramer of two A chains and two B chains.

Alternative Nomenclature

bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody

Coagulation factor XIII A chain antibody

F13A antibody

F13A1 antibody

Fibrin stabilizing factor, A subunit antibody

Fibrinoligase antibody

FSF, A subunit antibody

FXIIIA antibody

Protein-glutamine gamma-glutamyltransferase A chain antibody

TGase antibody

Transglutaminase A chain antibody

General Information

Product

IFI27 Antibody

Description

Affinity Purified Interferon, alpha-inducible protein 27 Antibody N-epitope

Verified Applications

ELISA, ICC/IF, IHC-Fr, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide: multiple antigenic peptide (8 branch) corresponding to amino acids 2-13 of Human IFI27.

Sequence:

EASALTSSAVTS

Accession #

NCBI:  NP_005523.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:100,000

ELISA

1:100,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI000019A7CD

Overview

Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.

Molecular Function

Lamin binding; RNA polymerase II activating transcription factor binding

Subcellular Location

Mitochondrion; Multi-pass membrane protein

Expression

Up-regulated by interferons

Structure

Belongs to the IFI6/IFI27 family.

Alternative Nomenclature

2310061N23Rik antibody

FAM14D antibody

IFI 27 antibody

Interferon alpha induced 11.5 kDa protein antibody

Interferon inducible protein 27 antibody

interferon-stimulated gene 12 antibody

ISG 12 antibody

ISG12A antibody

P27 antibody

General Information

Product

IFI27 Antibody Affinity Purified

Description

Affinity Purified Interferon, alpha-inducible protein 27, partial Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 69-119 on human Interferon alpha-inducible protein 27, mitochondrial isoform 2.

Accession #

NCBI:  NP_005523.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:100,000

ELISA

1:100,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

UPI000019A7CD

Overview

Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.

Molecular Function

Lamin binding; RNA polymerase II activating transcription factor binding

Subcellular Location

Mitochondrion; Multi-pass membrane protein

Expression

Up-regulated by interferons

Structure

Belongs to the IFI6/IFI27 family.

Alternative Nomenclature

2310061N23Rik antibody

FAM14D antibody

IFI 27 antibody

Interferon alpha induced 11.5 kDa protein antibody

Interferon inducible protein 27 antibody

interferon-stimulated gene 12 antibody

ISG 12 antibody

ISG12A antibody

P27 antibody

General Information

Product

MASPIN Antibody

Description

Affinity Purified Serpin B5 Antibody N-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on human MASPIN protein.

Accession #

GenBank:  AAA18957.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunofluorescence

1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P36952

Overview

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Molecular Function

Serine-type endopeptidase inhibitor activity

Subcellular Location

Secreted > extracellular space

Expression

Normal mammary epithelial cells.

Structure

Interacts with IRF6.

Alternative Nomenclature

mammary serine proteinase inhibitor antibody

Maspin antibody

Peptidase inhibitor 5 antibody

Protease inhibitor 5 antibody

Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody

SERPINB5 antibody

General Information

Product

MASPIN Antibody

Description

Affinity Purified Serpin B5 Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 310-360 on human MASPIN protein.

Accession #

GenBank:  AAA18957.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunofluorescence

1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P36952

Overview

Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Molecular Function

Serine-type endopeptidase inhibitor activity

Subcellular Location

Secreted > extracellular space

Expression

Normal mammary epithelial cells.

Structure

Interacts with IRF6.

Alternative Nomenclature

mammary serine proteinase inhibitor antibody

Maspin antibody

Peptidase inhibitor 5 antibody

Protease inhibitor 5 antibody

Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody

SERPINB5 antibody

General Information

Product

XTP4 Antibody

Description

Affinity Purified Chromosome17 orf 37 Antibody Extreme C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 80-130 on human XTP4 protein.

Accession #

GenBank: AF490253.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.83 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q9BRT3

Overview

Increases cell migration by induction of filopodia formation at leading edge.  Regulates apoptosis and redox-related process.

Molecular Function

Apoptosis

Subcellular Location

Cytoplasm; Cytosol; Cell membrane; Lipid anchor

Expression

Present in Leydig cells.  Up-regulated in breast cancers and brain cancer distant metastasis also up-regulated in prostate cancer cells and in the higher grades of prostate adenocarcinoma.

Structure

Belongs to SelWTH family

Alternative Nomenclature

C17orf37 antibody

C17orf37 homolog antibody

C35 antibody

chromosome 17 open reading frame 37 antibody

HBV X transactivated gene 4 protein antibody

HBV XAg transactivated protein 4 antibody

MGC14832 antibody

Mien1 antibody

Migration and invasion enhancer 1 antibody

ORB3 antibody

Protein C17orf37 antibody

Protein C35 antibody

RDX12 antibody

Redox protein, 12-KD antibody

XTP4 antibody

General Information

Product

XTP4 Antibody

Description

Affinity Purified Chromosome17 orf 37 Antibody N-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human XTP4 protein.

Accession #

GenBank: AF490253.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.83 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q9BRT3

Overview

Increases cell migration by induction of filopodia formation at leading edge.  Regulates apoptosis and redox-related process.

Molecular Function

Apoptosis

Subcellular Location

Cytoplasm; Cytosol; Cell membrane; Lipid anchor

Expression

Present in Leydig cells.  Up-regulated in breast cancers and brain cancer distant metastasis also up-regulated in prostate cancer cells and in the higher grades of prostate adenocarcinoma.

Structure

Belongs to SelWTH family

Alternative Nomenclature

C17orf37 antibody

C17orf37 homolog antibody

C35 antibody

chromosome 17 open reading frame 37 antibody

HBV X transactivated gene 4 protein antibody

HBV XAg transactivated protein 4 antibody

MGC14832 antibody

Mien1 antibody

Migration and invasion enhancer 1 antibody

ORB3 antibody

Protein C17orf37 antibody

Protein C35 antibody

RDX12 antibody

Redox protein, 12-KD antibody

XTP4 antibody