General Information |
|
Product |
cKit Peptide |
Description |
Antigenic Blocking Peptide Tyrosine-protein kinase Kit N-epitope |
Verified Applications |
Immunodepletion, Immunocompetition |
Peptide Description |
Synthetic peptide taken within amino acid region 130-180 on human Tyrosine-Kinase c-Kit protein. |
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|
Quantity |
250 µg |
Volume |
100 µl |
Form |
Antigenic Blocking Peptide |
Determinant |
N-epitope |
Storage |
-20⁰C for long term storage |
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|
Competition/Depletion assay |
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Uniprot # |
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Overview |
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1. Present in an inactive conformation in the absence of bound ligand. KITLG/SCF binding leads to dimerization and activation by autophosphorylation on tyrosine residues. Activity is down-regulated by PRKCA-mediated phosphorylation on serine residues. Inhibited by imatinib/STI-571 (Gleevec) and sunitinib; these compounds maintain the kinase in an inactive conformation. |
Molecular Function |
ATB binding; Cytokine binding; Metal ion binding; Stem cell factor receptor activity; Receptor signaling protein tyrosine kinase activity |
Subcellular Location |
Isoform 1 : Cell membrane; Single-pass type I membrane protein Isoform 2 : Cell membrane; Single-pass type I membrane protein Isoform 3 : Cytoplasm |
Expression |
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells. |
Structure |
Monomer in the absence of bound KITLG/SCF. Homodimer in the presence of bound KITLG/SCF, forming a heterotetramer with two KITLG/SCF molecules. Interacts (via phosphorylated tyrosine residues) with the adapter proteins GRB2 and GRB7 (via SH2 domain), and SH2B2/APS. Interacts (via C-terminus) with MPDZ (via the tenth PDZ domain). Interacts (via phosphorylated tyrosine residues) with PIK3R1 and PIK3 catalytic subunit. Interacts (via phosphorylated tyrosine) with CRK (isoform Crk-II), FYN, SHC1 and MATK/CHK (via SH2 domain). Interacts with LYN and FES/FPS. Interacts (via phosphorylated tyrosine residues) with the protein phosphatases PTPN6/SHP-1 (via SH2 domain), PTPN11/SHP-2 (via SH2 domain) and PTPRU. Interacts with PLCG1. Interacts with DOK1 and TEC. |
Alternative Nomenclature |
c-Kit antibody CD117 antigen antibody KIT antibody KIT oncogene antibody Mast cell growth factor receptor antibody p145 c-kit antibody PBT antibody Piebald trait protein antibody Proto oncogene c Kit antibody SCF Receptor antibody SCFR antibody soluble KIT variant 1 antibody Steel Factor Receptor antibody Stem cell factor receptor antibody Tyrosine-protein kinase Kit antibody v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody |
Protein information supplied by UniProt
Product | Note | Status | Price | |
---|---|---|---|---|
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CKIT-101AP | |||
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CKIT.101-FITC | |||
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CKIT.101-BIOTIN | |||
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PC-CKIT | |||
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