General Information

Product

CD86 Antibody Affinity Purified

Description

Affinity Purified T-lymphocyte activation antigen CD86 isoform 1 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 250-329 on human CD86 protein.

Accession #

NCBI:  NP_787058

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

P42081

Overview

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.

Molecular Function

Receptor; Adaptive Immunity; Host-virus interaction; Immunity

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Expressed by activated B-lymphocytes and monocytes.

Structure

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.  Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Alternative Nomenclature

Activation B7 2 antigen antibody

B-lymphocyte activation antigen B7-2 antibody

B7 2 antibody

B70 antibody

BU63 antibody

CD 86 antibody

CD28 antigen ligand 2 2 antibody

CLS1 antibody

CTLA 4 counter receptor B7 2 antibody

Early T-cell costimulatory molecule 1 antibody

ETC-1 antibody

FUN 1 antibody

LAB72 antibody

Ly-58 antibody

MB7-2 antibody

Membrane glycoprotein antibody

MGC34413 antibody

T-lymphocyte activation antigen CD86 antibody

TS/A-2 antibody

General Information

Product

CD163 Antibody

Description

Affinity Purified CD163 Antibody N-epitope

Verified Applications

ELISA, IHC, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 600-650  on human CD163 protein.

Accession #

GenBank: EAW88662.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunohistochemistry

1:150

Western Blot

1:500

Protein

Uniprot #

D3DUU0

Overview

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Molecular Function

Scavenger receptor activity

Subcellular Location

Membrane

Expression

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

Structure

Interacts with CSNK2B.

Alternative Nomenclature

CD163 antigen antibody

CD163 molecule antibody

Hemoglobin Scavenger Receptor antibody

Macrophage associated antigen antibody

MM130 antibody

Scavenger receptor cysteine rich type 1 protein M130 antibody

sCD163 antibody

Soluble CD163 antibody

General Information

Product

CD163 Antibody C-epitope

Verified Applications

ELISA, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region  887-905 on human CD163 protein.

Accession #

GenBank: EAW88662.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

D3DUU0

Overview

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

Molecular Function

Scavenger receptor activity

Subcellular Location

Membrane

Expression

Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

Structure

Interacts with CSNK2B.

Alternative Nomenclature

CD163 antigen antibody

CD163 molecule antibody

Hemoglobin Scavenger Receptor antibody

Macrophage associated antigen antibody

MM130 antibody

Scavenger receptor cysteine rich type 1 protein M130 antibody

sCD163 antibody

Soluble CD163 antibody

General Information

Product

CD19 Antibody

Description

Affinity Purified B-lymphocyte antigen CD19 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 200-250 on human CD19 protein.

Accession #

NCBI: AAB60697

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P15391

Overview

Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor dependent stimulation

Molecular Function

Receptor Signaling protein activity

Subcellular Location

Membrane; Single-pass type I membrane protein.

Structure

Contains 2 Ig-like C2-type domains.

Alternative Nomenclature

Antibody deficiency due to defect in CD19, included antibody

AW495831 antibody

CVID3 antibody

Leu 12 antibody

Lymphocyte Surface Antigen antibody

T-cell surface antigen Leu-12 antibody

General Information

Product

CD2 Antibody

Description

Affinity Purified T-cell surface antigen CD2 Antibody C-epitope

Verified Applications

ELISA, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Predicted: Mouse, Rat

Immunogen

Accession #

NCBI:  NP_001758

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P06729

Overview

Interacts with lymphocyte function associated antigen and CD48/BCM1 to mediate adhesion between T-cells and other cells.

Molecular Function

Cell adhesion

Subcellular Location

Membrane; Single-pass type I membrane protein.

Structure

Cytoplasmic domain is implicated in signaling function.  Contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain

Alternative Nomenclature

CD 2 antibody

CD2 antigen (p50), sheep red blood cell receptor antibody

CD2 antigen antibody

CD2 molecule antibody

Erythrocyte receptor antibody

FLJ46032 antibody

LFA2 antibody

LFA3 receptor antibody

Ly-37 antibody

Lymphocyte function antigen 2 antibody

OTTHUMP00000024366 antibody

p50 antibody

Rosette receptor antibody

Sheep erythrocyte receptor antibody

Sheep red blood cell receptor antibody

SRBC antibody

T cell surface antigen CD2 antibody

T cell surface antigen T11/Leu 5 antibody

T11 antibody

General Information

Product

CD2 Antibody

Description

Affinity Purified T-cell surface antigen CD2 precursor Antibody Extreme C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Predicted: Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acid region 3335-351 on human T-cell surface antigen CD2 protein.

Accession #

NCBI:  NP_001758

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Extreme C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P06729

Overview

Interacts with lymphocyte function associated antigen and CD48/BCM1 to mediate adhesion between T-cells and other cells.

Molecular Function

Cell adhesion

Subcellular Location

Membrane; Single-pass type I membrane protein.

Structure

Cytoplasmic domain is implicated in signaling function.  Contains 1 Ig-like C2-type domain and 1 Ig-like V-type domain

Alternative Nomenclature

CD 2 antibody

CD2 antigen (p50), sheep red blood cell receptor antibody

Erythrocyte receptor antibody

FLJ46032 antibody

LFA-2 antibody

LFA-3 receptor antibody

Ly-37 antibody

Lymphocyte function antigen 2 antibody

p50 antibody

Rosette receptor antibody

Sheep erythrocyte receptor antibody

T cell surface antigen CD2 antibody

T cell surface antigen T11/Leu 5 antibody

General Information

Product

CD21 Antibody

Description

Affinity Purified Complement Receptor Type 2 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Predicted: Rat

Immunogen

Synthetic peptide taken within amino acid region 650-700  on human Complement Receptor type 2 isoform X1 protein.

Accession #

NCBI:  XP_011507508.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P20023

Overview

Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.

Molecular Function

Complement binding; DNA binding; Protein homodimerization activity

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Structure

Interacts (via Sushi domain 1 and 2) with C3dg.

Alternative Nomenclature

C3DR antibody

CD21 antibody

CD21 antigen antibody

Complement C3d receptor antibody

Complement component (3d/Epstein Barr virus) receptor 2 antibody

Complement component receptor 2 antibody

Cr 2 antibody

CR antibody

CVID7 antibody

EBV receptor antibody

EBV-R antibody

Epstein Barr virus receptor antibody

EVBR antibody

SLEB9 antibody

General Information

Product

CD21 Antibody

Description

Affinity Purified Complement Receptor Type 2 Antibody C-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Predicted: Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 915-965  on human Complement Receptor type 2 protein.

Accession #

NCBI:  XP_011507508.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P20023

Overview

Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.

Molecular Function

Complement binding; DNA binding; Protein homodimerization activity

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Structure

Interacts (via Sushi domain 1 and 2) with C3dg.

Alternative Nomenclature

C3DR antibody

CD21 antibody

CD21 antigen antibody

Complement C3d receptor antibody

Complement component (3d/Epstein Barr virus) receptor 2 antibody

Complement component receptor 2 antibody

Cr 2 antibody

CR antibody

CVID7 antibody

EBV receptor antibody

EBV-R antibody

Epstein Barr virus receptor antibody

EVBR antibody

SLEB9 antibody

General Information

Product

CD3 epsilon Antibody

Description

Affinity Purified T-cell surface glycoprotein CD3 epsilon chain precursor Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequence on Human CD3 epsilon protein.

Accession #

NCBI:  NP_000724.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P07766

Overview

Mediates signal transduction

Molecular Function

Receptor; GPCR signaling pathway

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer coexprassed at the cell surface with invariant subunits of CD3 labeled gamma, delta epsilon, zeta, eta.

Alternative Nomenclature

CD3 TCR complex antibody

IMD18 antibody

T cell antigen receptor complex epsilon subunit of T3 antibody

T-cell surface antigen T3/Leu-4 epsilon chain antibody

T3E antibody

TCRE antibody

TiT3 complex antibody

General Information

Product

CD3 epsilon Antibody

Description

Affinity Purified T-cell surface glycoprotein CD3 epsilon chain Antibody Mid-region

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Acomys cahirinus, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 150-200 on human CD3 epsilon protein.

Accession #

NCBI:  NP_000724.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Western Blot

1:500-1:1,000

Protein

Uniprot #

P07766

Overview

Mediates signal transduction

Molecular Function

Receptor; GPCR signaling pathway

Subcellular Location

Membrane; Single-pass type I membrane protein

Structure

Heterodimer coexprassed at the cell surface with invariant subunits of CD3 labeled gamma, delta epsilon, zeta, eta.

Alternative Nomenclature

CD3 TCR complex antibody

IMD18 antibody

T cell antigen receptor complex epsilon subunit of T3 antibody

T-cell surface antigen T3/Leu-4 epsilon chain antibody

T3E antibody

TCRE antibody

TiT3 complex antibody