PDE4B Antibody

Product no.: PD4B-201AP

General Information
Product	PDE4B Antibody
Description	Affinity Purified cAMP-specific 3',5'-cyclic phosphodiesterase 4B Antibody N-epitope
Verified Applications	CM, ELISA, ICC, IF, IHC, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Mouse, Rat
Immunogen	Synthetic peptide common to all PDE4B subtypes taken near N-terminal
Specificity	This antibody detects all PDE4B proteins, including PDE4B1, PDE4B2, PDE4B3, and a 66 kDa PDE4B variant. It does not cross-react with other PDE family members such as PDE4A, PDE4C. PD4B-201AP may have minor crossreactivity to PDE4D. Antibody PD4B-212AP does not have any cross reactivity with any other PDE family members other than: PDE4B1, PDE4B2, PDE4B3, PDE4B4.
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.68 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	N-epitope
Storage	-20⁰C for long term storage
Application Protocol
Confocal Microscopy	1:250
DB	1:10,000
ELISA	1:10,000
Immunocytochemistry	1:250
Immunofluorescence	1:250
Immunohistochemistry	1:250
Immunoprecipitation	1:350
Western Blot	1:500-1:1,000
Protein
Uniprot #	Q07343
Overview	Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Molecular Function	Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Expression	Expressed in brain, heart, lung and skeletal muscle.
Alternative Nomenclature	5''-cyclic phosphodiesterase 4B antibody cAMP-specific 3'' antibody cAMP-specific 3',5'-cyclic phosphodiesterase 4B antibody DPDE4 antibody dunce-like phosphodiesterase E4 antibody MGC126529 antibody PDE 4B antibody PDE IVB antibody PDE32 antibody PDE4B5 antibody PDEIVB antibody Phosphodiesterase 4B antibody Phosphodiesterase-4B cAMP-specific (dunce (Drosophila)-homolog) antibody

Protein information supplied by UniProt

Fabgennix PDE4B Antibody has been used in 42 Publications
Kedia, George T., et al. "Expression and Distribution of Phosphodiesterase Isoenzymes in the Human Male Urethra." Urology (2015). PMID25704994	Immunofluorescence, Western Blot; *Human*
Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015). PMID25578602	Immunohistochemistry; *Human*
Guo, Jiejie, et al. "Etazolate abrogates the lipopolysaccharide (LPS)-induced downregulation of the cAMP/pCREB/BDNF signaling, neuroinflammatory response and depressive-like behavior in mice." Neuroscience 263 (2014): 1-14. PMID24434771	Western Blot; *Mouse*
Zhai, Kui, et al. "Phosphodiesterase types 3 and 4 regulate the phasic contraction of neonatal rat bladder smooth myocytes via distinct mechanisms." Cellular signalling 26.5 (2014): 1001-1010.PMID24463006	Western Blot; *Human*
Hiramoto, Kenichi, et al. "Role of phosphodiesterase 2 in growth and invasion of human malignant melanoma cells." Cellular signalling 26.9 (2014): 1807-1817. PMID24705027	Western Blot; *Rat*
Wang, Zhen-Zhen et al. “RNA Interference-Mediated Phosphodiesterase 4D Splice Variants Knock-down in the Prefrontal Cortex Produces Antidepressant-Like and Cognition-Enhancing Effects.” British Journal of Pharmacology 168.4 (2013): 1001–1014. PMID23003922	Western Blot; *Mouse*
Sanabra, Cristina, Emily M. Johansson, and Guadalupe Mengod. "Critical role for PDE4 subfamilies in the development of experimental autoimmune encephalomyelitis." Journal of chemical neuroanatomy 47 (2013): 96-105. PMID23274837	Immunohistochemistry; *Mouse*
Yougbaré, Issaka, et al. "NCS 613 Exhibits Anti-Inflammatory Effects On Pbmcs From Lupus Patients By Inhibiting P38 MAPK And NF-Κb Signalling Pathways While Reducing Proinflammatory Cytokine Production." Canadian Journal Of Physiology & Pharmacology 91.5 (2013): 353-361. PMID23656347	Immunocytochemistry, Western Blot; *Human*
Haque, Mohammed Z., Paulo S. Caceres, and Pablo A. Ortiz. “β-Adrenergic Receptor Stimulation Increases Surface NKCC2 Expression in Rat Thick Ascending Limbs in a Process Inhibited by Phosphodiesterase 4.” American Journal of Physiology - Renal Physiology 303.9 (2012): F1307–F1314. PMC3518189	Western Blot; *Rat*
Johansson, Emily M., Cristina Sanabra, and Guadalupe Mengod. "Sex‐related differences of cAMP‐specific PDE4B3 mRNA in oligodendrocytes following systemic inflammation." Glia 60.12 (2012): 1815-1825. PMID22848007	Western Blot; *Mouse*

For a complete listing of FabGennix PDE4B publications, please click here.

Publishing research using PD4B-201AP? Please let us know, we can cite the reference in this datasheet.

Could you please provide me with the immunogen sequence for Anti-PDE4B antibody (PD4B-201AP)

If it is not possible to provide me with the immunogen sequence itself, could you please send me the amino acid range that the immunogen is from?

Our PDE4B Antibody (PD4B-201AP), was developed using a recombinant fragment for PDE4B comprising of both UCR1 and UCR2 regions of the long-form variants. The antibody labels all PDE4B variants and has minor cross reactivity to PDE4D. We also have another C-terminal epitope PDE4B selective antibody (PD4B-212AP) that has no cross reactivity to any other PDE4 family members except PDE4B1, PDE4B2, PDE4B3, and PDE4B4.

In addition to these two, we also have antibodies that are selective for PDE4B subtype variants (PDE4B1, PDE4B2, PDE4B3, and PDE4B4) for studying individual variants of the PDE4B family members.

Customer ratings for PDE4B Antibody

Number of ratings: 1
Average rating: 4

Good for western blot
from Anonymous on 12/09/2020

Great results in western blot using rat cell lysate (Mesangial cells). 1/5000 primary antibody dilution for 8 hours.

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PDE4B Antibody FITC

PD4B-FITC

PDE4B Antibody BIOTIN

PD4B-BIOTIN

PDE4B Blocking Peptide

P-PD4B

PDE4B Positive Control

PC-PD4B

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