General Information

Product

MyD88 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 140-190 on human MyD88 protein.

Accession #

GenBank: AAC50954.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.8 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q99836

Overview

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response.

Molecular Function

death receptor binding;TIR domain binding

Subcellular Location

Cytoplasm

Expression

Ubiquitous

Structure

Homodimer. Also forms heterodimers with TIRAP

Alternative Nomenclature

MYD 88 antibody

Myeloid differentiation marker 88 antibody

General Information

Product

SHC Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 425-474 on mouse ShcA protein.

Accession #

NCBI:  NP_033193.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q61120

Overview

Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span. Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.

Molecular Function

Angiogenesis, Growth regulation

Subcellular Location

Cytoplasm

Expression

Widely expressed. Expressed in neural stem cells but absent in mature neurons.

Structure

Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. Once activated, binds to GRB2. Interacts with tyrosine-phosphorylated CD3T and DDR2. Interacts with N-terminal region of APS. Interacts with phosphorylated LRP1 and IRS4.Interacts with INPP5D/SHIP1 and INPPL1/SHIP2. Interacts with TRIM31. Interacts with PTPN6/SHP (tyrosine phosphorylated). Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTT. Interacts with ALK, GAB2, GRB7 and KIT. Interacts with FLT4 (tyrosine-phosphorylated). Interacts with EPHB1 and GRB2; activates the MAPK/ERK cascade to regulate cell migration. Interacts with PDGFRB (tyrosine-phosphorylated). Interacts with ERBB4. Interacts with TEK/TIE2 (tyrosine-phosphorylated). Interacts with Trk receptors NTRK1, NTRK2, and NTRK3; in a phosphotyrosine-dependent manner. Interacts with PTK2/FAK1.

Alternative Nomenclature

p46 antibody

p52 antibody

p66 antibody

SH2 domain protein C1 antibody

Shc antibody

SHC-transforming protein 1 antibody

SHC-transforming protein 3 antibody

SHC-transforming protein A antibody

SHC1 antibody

SHCA antibody

Src homology 2 domain containing transforming protein C1 antibody

General Information

Product

STING / TMEM173 Antibody

Description

Affinity Purified Stimulator of interferon genes protein isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acid region 332-342. The selected peptide was post-synthetically modified to achieve highest antigenicity before coupling to a carrier protein using hetero bifunctional cross linker.

Sequence:

SFSLLSSQEVLRHLLRQEEKEEVT

Accession #

NCBI:  NP_938023

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

86WV6

Overview

Facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm.

Molecular Function

cyclic-di-GMP binding, cyclic-GMP-AMP binding, identical protein binding

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasm › perinuclear region. Cytoplasm

Expression

Ubiquitously expressed. Expressed in skin endothelial cells, alveolar type 2 pneumocytes, bronchial epithelium and alveolar macrophages.

Structure

Associates with the MHC-II complex (By similarity). Homodimer; 'Lys-63'-linked ubiquitination at Lys-150 is required for homodimerization. Interacts with DDX58/RIG-I, MAVS and SSR2. Interacts with RNF5 and TRIM56. Interacts with TBK1; when homodimer, leading to subsequent production of IFN-beta. Interacts with IFIT1 and IFIT2.

Alternative Nomenclature

endoplasmic reticulum IFN stimulator antibody

ERIS antibody

FLJ38577 antibody

Mediator of IRF3 activation antibody

MITA antibody

Mitochondrial mediator of IRF3 activation antibody

MPYS antibody

N terminal methionine proline tyrosine serine plasma membrane tetraspanner antibody

NET23 antibody

Stimulator of interferon genes antibody

STING antibody

Tmem173 antibody

Transmembrane protein 173 antibody

General Information

Product

TLR12 Antibody Affinity Purified

Description

Affinity Purified Toll-like receptor 12 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Chimpanzee, Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 440-470 from mouse TLR12 protein.

Accession #

NCBI:  NP_991392.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.69 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500

Protein

Uniprot #

Q6QNU9

Overview

Participates in the innate immune response to microbial agents. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity).

Molecular Function

Receptor

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Macrophages, liver, kidney and bladder epithelial cells.

Structure

Binds MYD88 via their respective TIR domains.

Alternative Nomenclature

Toll-like receptor 12

General Information

Product

SOCS3 Antibody

Description

Affinity Purified Suppresor of Cytokine Signaling 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 175-225 on human SOCS3 protein.

Accession #

GenBank:  CAG46495

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q6FI39

Molecular Function

Protein modification; protein ubiquitination.

Subcellular Location

Cytoplasm

Expression

Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Alternative Nomenclature

ATOD4 antibody

CIS 3 antibody

Cish3 antibody

Cytokine induced SH2 protein 3 antibody

E2a Pbx1 target gene in fibroblasts 10 antibody

EF 10 antibody

SOCS 3 antibody

SSI3 antibody

STAT induced STAT inhibitor 3 antibody

Suppressor of cytokine signaling 3 antibody

General Information

Product

TRIF Antibody 

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on TRIF protein.

Accession #

GenBank:  BAA76376.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q9CQE0

Overview

E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway (By similarity).

Molecular Function

Ligase, Zinc ion binding

Subcellular Location

Nucleus

Structure

Interacts with NLK (via C-terminus).

Alternative Nomenclature

MGC35334 antibody

Proline rich vinculin and TIR domain containing protein B antibody

Proline-rich antibody

PRVTIRB antibody

Putative NF kappa B activating protein 502H antibody

Putative NFkB activating protein antibody

TICAM 1 antibody

TIR domain-containing adapter protein inducing IFN-beta antibody

Toll interleukin 1 receptor domain containing adapter protein inducing interferon beta antibody

Toll like receptor adaptor molecule 1 antibody

Toll-interleukin-1 receptor domain-containing adapter protein inducing interferon beta antibody

TRIF antibodyvinculin and TIR domain-containing protein B antibody

General Information

Product

TICAM2 Antibody

Description

Affinity Purified TRIF-related adapter molecule Antibody

Verified Applications

DB, ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on TRIF-related adapter molecule.

Accession #

GenBank: AAO74498.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000

ELISA

1:20,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q86XR7

Overview

Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4. May also be involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB; however, reports are controversial. Involved in IL-18 signaling and is proposed to function as a sorting adaptor for MYD88 in IL-18 signaling during adaptive immune response.

Molecular Function

phospholipid binding, signal transducer activity

Subcellular Location

Isoform 1 : Cytoplasm. Golgi apparatus. Cell membrane. Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum.

Expression

Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).

Structure

Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs i

Alternative Nomenclature

Putative NF-kappa-B-activating protein 502 antibody

TICAM2 antibody

TIR containing adapter molecule 2 antibody

TIR domain-containing adapter molecule 2 antibody

TIRAP3 antibody

TIRP antibody

Toll-like receptor adaptor protein 3 antibody

Toll/interleukin-1 receptor domain-containing protein antibody

TRAM antibody

TRIF-related adapter molecule antibody

General Information

Product

SOCS1 Antibody

Description

Affinity Purified Suppressor of Cytokine Signaling 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 170-212 on human Suppressor of Cytokine signaling 1 protein.

Accession #

GenBank: CAA27974.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P13848

Overview

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. SOCS1 appears to be a negative regulator in IGF1R signaling pathway.

Molecular Function

Insulin-like  growth factor receptor binding; Protein kinase binding; Kinase inhibitor activity

Subcellular Location

Nucleus; Cytoplasmic vesicle

Expression

Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.

Structure

Interacts with multiple activated signaling proteins of the tyrosine kinase signaling pathway including JAK family kinases, TEC, KIT, GRB2 and VAV. Binding to JAKs is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK JH1 domain. Binds the SH3 domain of GRB2 via diproline determinants in the N-terminus, and the N-terminal regulatory domain of VAV. Interacts with the Elongin BC complex (TCEB1 and TCEB2). Component of an ECS CBC (SOCS1) E3 ubiquitin-protein ligase complex which contains Elongin BC, CUL5, RBX1 and SOCS1. Interacts (via SH2 domain and SOCS box) with TRIM8. Interacts with AXL, CUL2 and FGFR3. Interacts with INSR.

Alternative Nomenclature

CISH 1 antibody

Cytokine Inducible SH2 Protein 1 antibody

JAB antibody

JAK-binding protein antibody

Janus kinase binding protein antibody

SOCS-1 antibody

SSI1 antibody

STAT Induced STAT Inhibitor 1 antibody

Suppressor of cytokine signaling 1 antibody

TEC Interacting Protein 3 antibody

TIP3 antibody

General Information

Product

Phospho-TICAM2 Antibody

Description

Affinity Purified Phosphorylated TRIF-related adapter molecule Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on TRIF-related adapter molecule protein.

Accession #

GenBank: AAO74498.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q86XR7

Overview

Functions as sorting adapter in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1; signaling is proposed to occur in early endosomes after endocytosis of TLR4.

Molecular Function

phospholipid binding, signal transducer activity

Subcellular Location

Isoform 1 : Cytoplasm. Golgi apparatus. Cell membrane. Endoplasmic reticulum. Early endosome membrane. Late endosome membrane, Isoform 2 : Endoplasmic reticulum. Early endosome membrane. Late endosome membrane.

Expression

Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).

Structure

Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs in late endosomes and dirupts the interaction between isoform 1 and TICAM1. Interacts with MYD88; the interaction decreases after IL-18 stimulation in time-dependent manner. Interacts with IL18R1 and IL8RAP.

Alternative Nomenclature

Putative NF-kappa-B-activating protein 502 antibody

TIR containing adapter molecule 2 antibody

TIR domain containing adaptor protein antibody

TIRAP3 antibody

TIRP antibody

Toll-like receptor adaptor protein 3 antibody

Toll/interleukin-1 receptor domain-containing protein antibody

TRAM antibody

TRIF-related adapter molecule antibody

General Information

Product

SHANK3 Antibody

Description

Affinity Purified SH3 and multiple ankyrin repeat domains protein 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1600-1650 on mouse SH3 and multiple ankyrin repeat domains protein 3.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q4ACU6

Overview

Major scaffold postsynaptic density protein which interacts with multiple proteins and complexes to orchestrate the dendritic spine and synapse formation, maturation and maintenance. Interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and HOMER, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction through the interaction with Arp2/3 and WAVE1 complex as well as the promotion of the F-actin clusters. By way of this control of actin dynamics, participates in the regulation of developing neurons growth cone motility and the NMDA receptor-signaling. Also modulates GRIA1 exocytosis and GRM5/MGLUR5 expression and signaling to control the AMPA and metabotropic glutamate receptor-mediated synaptic transmission and plasticity. May be required at an early stage of synapse formation and be inhibited by IGF1 to promote synapse maturation.

Molecular Function

GFAP/Homer scaffold activity, identical protein binding, ionotropic glutamate receptor binding, protein C-terminus binding, scaffold protein binding, SH3 domain binding, zinc ion binding

Subcellular Location

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

Expression

In brain, highly expressed in striatum, thalamus, hippocampus and granule cells of the cerebellum.

Structure

Belongs to the SHANK family.

Contains 6 ANK repeats.

Contains 1 PDZ (DHR) domain.

Contains 1 SAM (sterile alpha motif) domain.

Contains 1 SH3 domain.

Alternative Nomenclature

AI841104 antibody

DEL22q13.3 antibody

KIAA1650 antibody

Proline-rich synapse-associated protein 2 antibody

PSAP2 antibody

SH3 and multiple ankyrin repeat domains 3 antibody

SH3/ankyrin domain gene 3 antibody

SHAN3_HUMAN antibody

Shank postsynaptic density protein antibody

Shank3 antibody

Shank3b antibody

SPANK 2 antibody