General Information

Product

Myocilin Antibody C-epitope

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on Myocilin protein.

Accession #

GenBank: BAA24532.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q99972

Overview

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction.

Molecular Function

fibronectin binding, myosin light chain binding, frizzled binding

Subcellular Location

Secreted, Golgi apparatus, Cytoplasmic vessicle

Expression

Expressed in large amounts in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart, bone marrow-derived mesenchymal stem cells and other tissues. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and appeared more intensively than in normal eyes, regardless of the type or clinical severity of glaucoma.

Structure

Homodimer (via N-terminus). Interacts with OLFM3, FN1, NRCAM, GLDN and NFASC. Interacts (via N-terminus) with MYL2. Interacts with SFRP1, FRZB, FZD7, FZD10, FZD1 and WIF1; regulates Wnt signaling. Interacts with SNTA1; regulates muscle hypertrophy. Interacts with ERBB2 and ERBb3; activates ERBB2-ERBB3 signaling pathway. Interacts with SNCG; affects its secretion and aggregation.

Alternative Nomenclature

Glaucoma 1 open angle antibody

GLC1A antibody

GPOA antibody

JOAG antibody

Mutated trabecular meshwork-induced glucocorticoid response protein antibody

MYOC antibody

Myocilin antibody

TIGR antibody

General Information

Product

Myocilin Antibody

Verified Applications

ELISA, ICC/IF, IHC, IP, Immunomicroscopy, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide corresponding to N terminal amino acids 25-46 of Myocilin. Peptide was covalently modified and conjugated to KLH.

Sequence:

CLWDVGARTAQLRKANDQSGR

Accession #

GenBank: BAA24532.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75 µg/µl in antibody stabilization buffer

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500. Predicted molecular weight: 55 kDa.

Protein

Uniprot #

Q99972

Overview

Secreted glycoprotein regulating the activation of different signaling pathways in adjacent cells to control different processes including cell adhesion, cell-matrix adhesion, cytoskeleton organization and cell migration. Promotes substrate adhesion, spreading and formation of focal contacts. Negatively regulates cell-matrix adhesion and stress fiber assembly through Rho protein signal transduction.

Molecular Function

fibronectin binding, myosin light chain binding, frizzled binding

Subcellular Location

Secreted, Golgi apparatus, Cytoplasmic vessicle

Expression

Expressed in large amounts in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart, bone marrow-derived mesenchymal stem cells and other tissues. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and appeared more intensively than in normal eyes, regardless of the type or clinical severity of glaucoma.

Structure

Homodimer (via N-terminus). Interacts with OLFM3, FN1, NRCAM, GLDN and NFASC. Interacts (via N-terminus) with MYL2. Interacts with SFRP1, FRZB, FZD7, FZD10, FZD1 and WIF1; regulates Wnt signaling. Interacts with SNTA1; regulates muscle hypertrophy. Interacts with ERBB2 and ERBb3; activates ERBB2-ERBB3 signaling pathway. Interacts with SNCG; affects its secretion and aggregation.

Alternative Nomenclature

Glaucoma 1 open angle antibody

GLC1A antibody

GPOA antibody

JOAG antibody

Mutated trabecular meshwork-induced glucocorticoid response protein antibody

MYOC antibody

Myocilin antibody

TIGR antibody

General Information

Product

PDE6 delta / PDE6C Cone Specific Antibody

Description

Affinity Purified cone cGMP phosphodiesterase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-75 on cone-specific PDE6C protein.

Accession #

GenBank: CAA64079.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P51160

Overview

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side

Structure

Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.

Alternative Nomenclature

cGMP phosphodiesterase 6C antibody

COD4 antibody

PDE 6C antibody

PDE6 alpha prime antibody

PDEA2 antibody

Phosphodiesterase 6C cGMP specific cone alpha prime antibody

General Information

Product

Pan PDE6 Alpha Antibody 

Description

Affinity Purified Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha Antibody

Verified Applications

CM, ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic cyclic peptide common to all PDE6 Alpha variants

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:250

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:250

Immunofluorescence

1:250

Immunohistochemistry

1:250

Immunoprecipitation

1:250

Western Blot

1:500

Protein

Uniprot #

P51160

Molecular Function

Hydrolase, 3',5'-cyclic-GMP phosphodiesterase activity, cGMP binding

Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side

Structure

Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.

Alternative Nomenclature

5''-cyclic phosphodiesterase subunit alpha antibody

CGPR A antibody

GMP PDE alpha antibody

PDE 6 alpha antibody

PDE V B1 antibody

PDEA antibody

Phosphodiesterase 6 alpha antibody

Retinal Rod Photoreceptor cGMP

Rod cGMP specific 3' 5' cyclic phosphodiesterase alpha subunit antibody

General Information

Product

PDE6 Beta Antibody

Description

Affinity Purified Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta Antibody

Verified Applications

ELISA, CM, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Pig, Rat

Immunogen

Synthetic cyclic petide common to all PDE6 Beta variants

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Western Blot

1:500

Protein

Uniprot #

P35913

Overview

This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.

Molecular Function

Hydrolase, 3', 5' -cyclic-AMP phosphodiesterase activity

Subcellular Location

Membrane; lipid anchor

Structure

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Alternative Nomenclature

5''-cyclic phosphodiesterase subunit beta antibody

Congenital stationary night blindness 3 autosomal dominant antibody

CSNB 3 antibody

CSNBAD2 antibody

DPDE 4 antibody

GMP-PDE beta antibody

PDE 6 beta antibody

PDE 6B antibody

PDEB antibody

Phosphodiesterase 6B antibody

Rd 1 antibody

Rod cGMP specific 3' 5' cyclic phosphodiesterase beta subunit antibody

Rod cGMP-specific 3'' antibody

RP40 antibody

General Information

Product

PDE6 Gamma Antibody

Description

Affinity Purified Retinal cone Phosphodiesterase 6 gamma Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Synthetic cyclic peptide common to all PDE6 gamma variants.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Overview

Visual perception

Molecular Function

3',5'-cyclic-nucleotide phosphodiesterase activity, cGMP binding

Alternative Nomenclature

5''-cyclic phosphodiesterase subunit gamma antibody

GMP-PDE gamma antibody

PDE 6 antibody

PDEG antibody

Phosphodiesterase 6G cGMP Specific antibody

Retinal Rod Photoreceptor cGMP Phosphodiesterase gamma antibody

RP57 antibody

General Information

Product

Bestrophin 3 Antibody

Verified Applications

CM, ELISA, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within the last 50 amino acids from the C-terminus of rat Bestrophin-3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

Dot Blot

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q6H1V1

Overview

Forms calcium-sensitive chloride channels. Permeable to bicarbonate.

Molecular Function

Ion transport; Calcium, Chloride.

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Expression

Skeletal muscle and weakly in brain, spinal cord, bone marrow and retina.

Structure

Belongs to bestrophin Family

Alternative Nomenclature

BEST 3 antibody

Bestrophin3 antibody

MGC13168 antibody

MGC40411 antibody

Vitelliform macular dystrophy 2-like protein 3 antibody

VMD2L3 antibody

General Information

Product

Bestrophin 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey

Immunogen

Accession #

NCBI: NP_116124.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:200

Immunofluorescence

1:200

Immunohistochemistry

1:200

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q8N1M1

Overview

Forms calcium-sensitive chloride channels. Permeable to bicarbonate.

Molecular Function

Ion transport; Calcium, Chloride.

Subcellular Location

Cell membrane; Multi-pass membrane protein.

Expression

Skeletal muscle and weakly in brain, spinal cord, bone marrow and retina.

Structure

Belongs to bestrophin Family

Alternative Nomenclature

BEST 3 antibody

Bestrophin3 antibody

MGC13168 antibody

MGC40411 antibody

Vitelliform macular dystrophy 2-like protein 3 antibody

VMD2L3 antibody

General Information

Product

Bestrophin 4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 400-500 on Bestrophin 4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.20 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q8NFU0

Overview

Forms calcium-sensitive chloride channels. Permeable to bicarbonate.

Molecular Function

Chloride Channel

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Predominantly found in colon and the weakly in fetal brain, spinal cord, retina, lung, trachea, testis and placenta.

Structure

Belongs to bestrophin Family

Alternative Nomenclature

BEST 4 antibody

MGC126872 antibody

Vitelliform macular dystrophy 2 like 2 antibody

VMD2-like gene 2 antibody

VMD2L2 antibody

General Information

Product

Bestrophin 4 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, D. melanogaster

Immunogen

Synthetic peptide taken within amino acid region 400-500 on human Bestrophin 4 protein.

Accession #

NCBI:  NP_695006.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.20 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:2,000

ELISA

1:2,000

Immunoprecipitation

1:200

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q8NFU0

Overview

Forms calcium-sensitive chloride channels. Permeable to bicarbonate.

Molecular Function

Chloride Channel

Subcellular Location

Cell membrane; Multi-pass membrane protein

Expression

Predominantly found in colon and the weakly in fetal brain, spinal cord, retina, lung, trachea, testis and placenta.

Structure

Belongs to bestrophin Family

Alternative Nomenclature

BEST 4 antibody

MGC126872 antibody

Vitelliform macular dystrophy 2 like 2 antibody

VMD2-like gene 2 antibody

VMD2L2 antibody