General Information

Product

FUBP1 Antibody

Description

Affinity Purified Far upstream-element binding protein 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 600-644 on human FUBP1 protein.

Accession #

GenBank: AAH17247.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.53-0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q96AE4

Overview

Regulates the expression of MYC via binding to a single stranded far-upstream element upstream of the MYC promoter.  May act as both an activator and repressor of transcription.

Molecular Function

poly(A) RNA binding, single stranded DNA binding

Subcellular Location

Nucleus

Structure

Present in complex with PU560 and far upstream element DNA segment.  Interacts with PU560 and JTV1.

Alternative Nomenclature

DNA helicase V antibody

far upstream element (FUSE) binding protein 1 antibody

Far upstream element (FUSE) binding protein 4 antibody

FBP antibody

FUBP antibody

Fubp4 antibody

FUSE-binding protein 1 antibody

HDH V antibody

General Information

Product

MeCP2 isoform 2 Antibody

Description

Affinity Purified Methyl-CpG-binding protein 2 isoform 2 Antibody

Verified Applications

ELISA, ICC/IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 436-486 on Methyl-CpG-binding protein 2 isoform 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P51608

Overview

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC) .

Molecular Function

Chromatin binding, DNA binding, mRNA binding, double-stranded methylated DNA binding, poly(A) RNA binding, siRNA binding

Subcellular Location

Nucleus (Note: Colocalized with methyl-CpG in the genome)

Expression

Present in all adult somatic tissues tested.

Alternative Nomenclature

AUTSX 3 antibody

Mbd5 antibody

MeCP2 antibody

Methyl CpG binding protein 2 (Rett syndrome) antibody

MRX 16 antibody

MRX 79 antibody

MRXS 13 antibody

MRXSL antibody

PPMX antibody

RS antibody

RTS antibody

RTT antibody

WBP10 antibody

General Information

Product

MeCP2 isoform 1 Antibody

Description

Affinity Purified Methyl-CpG-binding protein 2 isoform 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1-50 on mouse methyl-CpG-binding protein 2 isoform 1.

Accession #

NCBI:  NP_004983

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P51608

Overview

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

Molecular Function

Chromatin binding, DNA binding, mRNA binding, double-stranded methylated DNA binding, poly(A) RNA binding, siRNA binding

Subcellular Location

Nucleus (Note: Colocalized with methyl-CpG in the genome)

Structure

Interacts with FNBP3. Interacts with CDKL5 (PubMed:15917271). Interacts with ATRX; MECP2 recruits ATRX to pericentric heterochromatin in neuronal cells. Interacts with NCOR2.

Expression

Present in all adult somatic tissues tested.

Alternative Nomenclature

AUTSX 3 antibody

Mbd5 antibody

MeCP-2 protein antibody

MeCP2 antibody

Methyl CpG binding protein 2 (Rett syndrome) antibody

Methyl CpG binding protein 2 antibody

MRX 16 antibody

MRX 79 antibody

MRXS13 antibody

MRXSL antibody

PPMX antibody

RS antibody

RTS antibody

RTT antibody

WBP 10 antibody

General Information

Product

MEDEA Antibody Affinity Purified

Description

Affinity Purified Medea Antibody

Verified Applications

DB, ELISA, WB

Host

Rabbit

Species Cross Reactivity

Drosophila melanogaster

Immunogen

Synthetic peptide taken within amino acid region 150-250 on Drosophila melanogaster Medea protein.

Accession #

GenBank: AAC62005.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.73 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

Q7KPL1

Overview

Sequence-specific DNA binding transcription factor activity

Molecular Function

Transforming growth factor beta receptor, common-partner cytoplasmic mediator activity

Subcellular Location

Cytoplasm;Nucleus

Structure

Belongs to the dwarfin/SMAD family.

Alternative Nomenclature

(Small) Mothers Against Decapentaplegic antibody

Deleted in Pancreatic Carcinoma 4 antibody

DPC 4 antibody

hSMAD4 antibody

JIP antibody

MAD mothers against decapentaplegic homolog 4 antibody

MADH 4 antibody

Med antibody

Medea antibody

MYHRS antibody

Smad 4 antibody

General Information

Product

Phospho NT5C2 Antibody Affinity Purified

Description

Affinity Purified Phospho 5'-nucleotidase, cytosolic II Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on cNII protein with phosphorylated Serine 7.

Accession #

GenBank: CAI40080

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.85 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P49902

Overview

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides.

Molecular Function

Magnesium, Metal-binding, Nucleotide-binding

Subcellular Location

Cytoplasm

Structure

Homotetramer

Alternative Nomenclature

5' nucleotidase (purine) cytosolic type B antibody

cN II antibody

Cytosolic 5''-nucleotidase II antibody

Cytosolic IMP/GMP-specific 5'-nucleotidase antibody

GMP antibody

NT5B antibody

NT5CP antibody

NUCLEOTIDASE, 5-PRIME, TYPE B antibody

PNT5 antibody

PURINE 5-PRIME-NUCLEOTIDASE antibody

General Information

Product

U2AF59 Antibody

Description

Affinity Purified Splicing factor U2AF 59 kDa subunit Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Saccharomyces cerevisiae (yeast)

Immunogen

Synthetic peptide taken within amino acid region 480-517 on yeast U2AF59 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-1.13 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500-1:750

Protein

Uniprot #

P36629

Overview

Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.

Molecular Function

mRNA binding, snoRNA binding, nucleotide binding

Subcellular Location

Nucleus

Structure

Forms a heterodimer with the U2AF small subunit. Can also form a homodimer. U2AF large subunit (U2AF59), U2AF small subunit (U2AF23) and SF1 (bpb1) interact to form a complex required for complex A formation. Interacts with wat1/pop3.

Alternative Nomenclature

S. pombe splicing factor U2 59 kDa subunit

Schzosacharomyces pombe splicing factor U2 auxiliary factor

U2 auxiliary factor 59 kDa subunit

U2 snRNP auxiliary factor large subunit

mis11

prp2

General Information

Product

C6orf150 Antibody

Description

Affinity Purified Cyclic GMP-AMP synthase Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on mouse cGAS protein.

Accession #

NCBI: NP_775562

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q8C6L5

Overview

Catalyzes the formation of cyclic GMP-AMP from ATP and GTP.  Has antiviral activity through acting as a cystolic DNA senser.

Molecular Function

Nucleotidyltransferase; ATP binding; DNA binding

Subcellular Location

Cytoplasm; Cytosol

Expression

Is expressed in the monocytic cell line THP1 by type 1 Interferons

Structure

Monomer when in absence of DNA.  Homodimer when bound to DNA.

Alternative Nomenclature

C6orf150 antibody

cGAMP synthase antibody

cGAS antibody

Chromosome 6 open reading frame 150 antibody

Cyclic GMP-AMP synthase antibody

h-cGAS antibody

Hypothetical protein LOC115004 antibody

Mab 21 domain containing 1 antibody

MB21D1 antibody

protein MB21D1 antibody

Uncharacterized protein C6orf150 antibody

General Information

Product

PELO Antibody

Description

Affinity Purified PELOTA Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-380 on human PELOTA protein.

Accession #

GenBank: AAG22575.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9BRX2

Overview

Is necessary in cell division for normal chromosome segregation and genomic stability.  It may recognize stalled ribosomes and trigger endonucleolytic cleavage of the mRNA, and may have ribonuclease activity.

Molecular Function

Endonuclease, Hydrolase, Nuclease

Subcellular Location

Nuclease, Cytoplasm

Expression

Expressed ubiquitously

Structure

The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity (Potential).  Belongs to the eukaryotic release factor 1 family. Pelota subfamily.

Alternative Nomenclature

CGI 17 antibody

pelo antibody

pelota (Drosophila) homolog antibody

pelota homolog antibody

PRO1770 antibody

Protein pelota homolog antibody

General Information

Product

PERK Antibody

Description

Affinity Purified Eukaryotic translation initiation factor 2-alpha kinase 3 Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on human Eukaryotic translation initiation factor 2-alpha kinase 3 protein.

Accession #

NCBI:  NP_004827

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9NZJ5

Overview

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Expression

Ubiquitous. A high level expression is seen in secretory tissues.

Structure

Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells. Interacts with DNAJC3 and MFN2.

Alternative Nomenclature

DKFZp781H1925 antibody

E2AK3_HUMAN antibody

EC 2.7.11.1 antibody

Eif2ak3 antibody

Eukaryotic translation initiation factor 2 alpha kinase 3 antibody

Heme regulated EIF2 alpha kinase antibody

HRI antibody

HsPEK antibody

Pancreatic eIF2 alpha kinase antibody

PEK antibody

PRKR like endoplasmic reticulum kinase antibody

WRS antibody

General Information

Product

Timeless Antibody

Description

Affinity Purified Protein timeless homolog isoform 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide:

SGTPRVHRKKRFQIEDEDD

coresponding to amino acids 1178-1197 on Mouse Timeless protein.

Accession #

NCBI:  NP_035719.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.98-1.26 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9R1X4

Overview

Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication and in the regulation of the circadian clock. Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock.

Molecular Function

Developmental protein, protein heterodimerization activity, protein homodimerization activity

Subcellular Location

Nucleus

Expression

Predominantly and robustly expressed in proliferative organs (spleen, thymus, intestine and testis) compared to those more differentiated such as kidney and liver (at protein level). Expressed in all tissues examined including brain, heart, lung, liver, skeletal muscle, kidney, placenta, pancreas, spleen, thymus and testis.

Structure

Homodimer or homomultimer. 

Alternative Nomenclature

Protein timeless homolog antibody

TIM antibody

timeless circadian clock 1 antibody

TIMELESS1 antibody

Tof1 homolog antibody