General Information

Product

ITPR2 Antibody

Description

Affinity Purified Inositol 1,4,5-triphosphate receptor type 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-150 on rat Inosital 1,4,5-triphosphate receptor type 2 protein.

Accession #

NCBI:  NP_112308

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.66 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P29995

Overview

Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. This release is regulated by cAMP both dependently and independently of PKA.

Molecular Function

Calcium ion binding; inositol 1,4,5-triphosphate binding; cellular response to hypoxia; Transport

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein

Expression

Isoform Short is found in skeletal muscle and heart.

Structure

The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.

Alternative Nomenclature

4 antibody

5-trisphosphate receptor antibody

Inositol 1 4 5 trisphosphate receptor type 2 antibody

InsP3R2 antibody

IP3 R2 antibody

IP3 receptor antibody

IP3 receptor isoform 2 antibody

IP3R 2 antibody

ITPR 2 antibody

Type 2 inositol 1 4 5 trisphosphate receptor antibody

Type 2 inositol 1 antibody

Type 2 InsP3 receptor antibody

General Information

Product

E-Cadherin Antibody

Description

Affinity Purified E-cadherin 1 Antibody Multi-Epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Multiple synthetic peptides taken within amino acid regions 50-100, 350-400, and 650-700 on human E-cadherin 1 protein.

Accession #

GenBank: ADC53120.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Multi-Epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

D3XNU5

Overview

Cadherins are calcium dependent cell adhesion proteins.

Molecular Function

Calcium ion binding;

Subcellular Location

Membrane; Single-pass type I  membrane protein

Structure

Repeat; Transmembrane helix

Alternative Nomenclature

Arc 1 antibody

Cadherin 1 antibody

cadherin 1 type 1 E-cadherin antibody

CAM 120/80 antibody

CD 234 antibody

CD 324 antibody

CDH1 antibody

CDHE antibody

E-Cad/CTF3 antibody

E-cadherin antibody

ECAD antibody

Epithelial cadherin antibody

epithelial calcium dependant adhesion protein antibody

LCAM antibody

Liver cell adhesion molecule antibody

UVO antibody

Uvomorulin antibody

General Information

Product

DWORF Antibody

Description

Affinity Purified Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-30 on mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P0DN83

Overview

Enhances the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by displacing ATP2A1/SERCA1 inhibitors, thereby acting as a key regulator of skeletal muscle activity. Does not directly stimulates SERCA pump activity. Enhances sarcoplasmic reticulum Ca2+ uptake and myocyte contractility by displacing the SERCA inhibitory peptides sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN).

Subcellular Location

Sarcoplasmic reticulum membrane; Single-pass membrane protein

Expression

Highly expressed in heart (at protein level). Detected in heart and soleus, a postural muscle group of the hindlimb containing the highest enrichment of slow-twitch muscle fibers. Also expressed in diaphragm, which contains some slow-twitch fibers. Not detected in the quadriceps, a fast-twitch muscle group, or in cardiac atrial muscle. Not expressed in the prenatal heart but gradually increases in abundance postnatally.

Alternative Nomenclature

Dwarf open reading frame antibody

DWORF antibody

Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF

SERCA regulator DWORF antibody

General Information

Product

Myoregulin Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Predicted: Mouse

Immunogen

Synthetic peptide taken within amino acid region 1-50 on human Myoregulin protein.

Accession #

NCBI:  NP_001291661

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P0DMT0

Overview

Inhibits the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca2+, thereby acting as a key regulator of skeletal muscle activity. Its high expression in adult skeletal muscle, suggests that it constitutes the predominant regulator of ATP2A1/SERCA1 in adult skeletal muscle.

Molecular Function

Enzyme inhibitor activity

Subcellular Location

Sarcoplasmic reticulum membrane, Single-pass membrane protein

Structure

Transmembrane, Transmembrane helix

Alternative Nomenclature

Myoregulin antibody
Long Intergenic Non-Protein Coding RNA 948 antibody
Muscle Enriched RNA antibody
Muscle Specific antibody
MLN antibody
LINC00948 antibody
MUSER1 antibody
M1 antibody

General Information

Product

DWORF Antibody

Description

Affinity Purified Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synththetic peptide corresponding to full length Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator DWORF protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P0DN83

Overview

Enhances the activity of ATP2A1/SERCA1 ATPase in sarcoplasmic reticulum by displacing ATP2A1/SERCA1 inhibitors, thereby acting as a key regulator of skeletal muscle activity. Does not directly stimulates SERCA pump activity. Enhances sarcoplasmic reticulum Ca2+ uptake and myocyte contractility by displacing the SERCA inhibitory peptides sarcolipin (SLN), phospholamban (PLN) and myoregulin (MRLN).

Molecular Function

Sarcoplasmic reticulum membrane; Single-pass membrane protein

Alternative Nomenclature

Dwarf open reading frame antibody

DWORF antibody

Sarcoplasmic/endoplasmic reticulum calcium ATPase regulator

DWORFSERCA regulator DWORF antibody

General Information

Product

DAP Kinase 1 Antibody

Description

Affinity Purified Death-associated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1370-1420 on human Death-associated protein kinase 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P53355

Overview

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy.

Molecular Function

ATP binding

calmodulin binding

calmodulin-dependent protein kinase activity

GTP binding

identical protein binding

protein kinase activity

protein serine/threonine kinase activity

syntaxin-1 binding

Subcellular Location

Cytoskeleton

Expression

Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Structure

The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Alternative Nomenclature

DAK1 antibody

DAP K1 antibody

DAP kinase 1 antibody

DAPK antibody

DAPK1 antibody

Death Associated Protein Kinase 1 antibody