General Information

Product

Lamin B1 antibody - Nuclear Envelope Marker Antibody

Description

Affinity Purified B1 antibody - Nuclear Envelope Marker Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Lamin-B1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P20700

Overview

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Molecular Function

phospholipase binding

structural molecule activity

Subcellular Location

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Expression

Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12.

Structure

Belongs to the intermediate filament family.

Alternative Nomenclature

ADLD antibody

lamin B1 antibody

LMN antibody

LMN2 antibody

LMNB antibody

Lmnb1 antibody

General Information

Product

DAP Kinase 1 Antibody

Description

Affinity Purified Death-associated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1370-1420 on human Death-associated protein kinase 1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P53355

Overview

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy.

Molecular Function

ATP binding

calmodulin binding

calmodulin-dependent protein kinase activity

GTP binding

identical protein binding

protein kinase activity

protein serine/threonine kinase activity

syntaxin-1 binding

Subcellular Location

Cytoskeleton

Expression

Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Structure

The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Alternative Nomenclature

DAK1 antibody

DAP K1 antibody

DAP kinase 1 antibody

DAPK antibody

DAPK1 antibody

Death Associated Protein Kinase 1 antibody

General Information

Product

DAPK2 Antibody

Description

Affinity Purified Death-associated protein kinase 2 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide take within amino acid region 300-350 on mouse Death-associated protein kinase 2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

Q8VDF3

Overview

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Capable of regulating both type I apoptotic and type II autophagic cell death signals.

Molecular Function

ATP binding

calmodulin binding

identical protein binding

protein serine/threonine kinase activity

Subcellular Location

Cytoplasm

autophagosome lumen

Expression

Expressed in peritubular interstitial cells of the renal cortex (PubMed:24906443). Isoform 1 is found in the adult brain while isoform 2 is expressed in brains of embryos and young mice (at protein level) (PubMed:21408167).

Structure

The autoinhibitory domain sterically blocks the substrate peptide-binding site by making both hydrophobic and electrostatic contacts with the kinase core.

Alternative Nomenclature

DAK2 antibody

DAP kinase 2 antibody

DAP kinase related protein 1 antibody

DAP kinase related protein 1 beta isoform antibody

DAP-kinase-related protein 1 antibody

DAPK2 antibody

Death associated protein kinase 2 antibody

DRP 1 antibody

General Information

Product

ZIP Kinase Antibody

Description

Affinity Purified Death-associated protein kinase 3 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region on 400-448 on mouse Death-associated protein kinase 3 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

O54784

Overview

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization.

Molecular Function

ATP binding

identical protein binding

kinase activity

leucine zipper domain binding

protein C-terminus binding

protein homodimerization activity

protein serine/threonine kinase activity

Rho GTPase binding

Subcellular Location

Cytoskeleton, Nucleus

Expression

Highly expressed in heart, brain, lung, skeletal muscle, kidney and testis. Lower levels in liver and spleen.

Structure

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.

Alternative Nomenclature

DAP kinase 3 antibody

DAP like kinase antibody

DAPK3 antibody

Death associated kinase 3 antibody

Death associated protein kinase 3 antibody

Dlk antibody

MYPT1 kinase antibody

ZIP antibody

ZIP kinase antibody

ZIP kinase isoform antibody

ZIPK antibody

zipper-interacting protein kinase antibody

General Information

Product

IFI6 Antibody

Description

Affinity Purified Interferon alpha-inducible protein 6 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide take within amino acid region 80-130 on human Interferon alpha-inducible protein 6.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P09912

Molecular Function

Up-regulated by type-I interferon (PubMed:14728724, PubMed:17823654). Up-regulated upon Dengue virus type 2/DENV2 infection (at protein level) (PubMed:26244642).

Subcellular Location

Mitochondrion

Expression

Up-regulated by type-I interferon (PubMed:14728724, PubMed:17823654). Up-regulated upon Dengue virus type 2/DENV2 infection (at protein level) (PubMed:26244642).

Structure

Belongs to the IFI6/IFI27 family.

Alternative Nomenclature

Ifi-6-16 antibody
IFI6 antibody
IFI6_HUMAN antibody
Interferon alpha-inducible protein 6 antibody
Interferon-induced protein 6-16 antibody

General Information

Product

MLKL Antibody

Description

Affinity Purified Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 400-450 on mouse Mixed lineage kinase domain-like protein MLKL.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q9D2Y4

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414). Within this complex, may play a role in the proper targeti

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody

General Information

Product

Phospho-MLKL Antibody

Description

Affinity Purified Phosphorylated Mixed lineage kinase domain-like protein MLKL Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 350-400 on human Mixed lineage kinase domain-like protein MLKL

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Modification

Phosphorylated

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q8NB16

Overview

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Does not have protein kinase activity (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:22265413, PubMed:22265414, PubMed:22421439, PubMed:24316671).

Molecular Function

ATP binding

identical protein binding

JUN kinase kinase kinase activity

protein-containing complex binding

protein kinase activity

protein kinase binding

Subcellular Location

Nucleus, Plasma membrane, cytoplasm

Structure

Homooligomer (PubMed:29883610). Homotrimer; forms homotrimers on necroptosis induction (PubMed:24316671). Upon TNF-induced necrosis, forms in complex with PGAM5, RIPK1 and RIPK3 (PubMed:22265414).

Alternative Nomenclature

9130019I15Rik antibody

FLJ34389 antibody

hMLKL antibody

Mixed lineage kinase domain like antibody

Mixed lineage kinase domain like pseudokinase antibody

Mixed lineage kinase domain-like protein antibody

Mlkl antibody

MLKL_HUMAN antibody

General Information

Product

Humanin Antibody

Verified Applications

ELISA, IP, WB

Host

Chicken

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to full length Humanin peptide sequence.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgY

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

Full length peptide

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q8IVG9

Overview

Plays a role as a neuroprotective factor. Protects against death induced by multiple different familial Alzheimer disease genes and beta amyloid proteins in Alzheimer disease. Suppresses apoptosis by binding to BAX and preventing the translocation of BAX from the cytosol to mitochondria. Binds to IGFBP3 and specifically blocks IGFBP3-induced cell death Induces chemotaxis of mononuclear phagocytes via FPR2. Reduces the aggregation and fibrillary formation by suppressing the effect of APP on mononuclear phagocytes and acts by competitively inhibiting the access of FPRL1 to APP.

Molecular Function

Receptor antagonist activity; Apoptosis; Leukocyte chemotaxis

Subcellular Location

Secreted; Cytoplasm

Expression

Expressed in the heart, skeletal muscles, kidney and liver. Lesser but significant expression is observed in the brain and the gastrointestinal tract. Expressed in the AD brain, where it is found in some of the large intact neurons of the occipital lobes and small and round reactive glial cells in the hippocampus.

Structure

Largely unstructured in aqueous solution.

Alternative Nomenclature

HN antibody

HUNIN Antibody

MT-RNR2 antibody

General Information

Product

Humanin Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide corresponding to full length Humanin peptide sequence.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

Full length peptide

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

Q8IVG9

Overview

Plays a role as a neuroprotective factor. Protects against death induced by multiple different familial Alzheimer disease genes and beta amyloid proteins in Alzheimer disease. Suppresses apoptosis by binding to BAX and preventing the translocation of BAX from the cytosol to mitochondria. Binds to IGFBP3 and specifically blocks IGFBP3-induced cell death Induces chemotaxis of mononuclear phagocytes via FPR2. Reduces the aggregation and fibrillary formation by suppressing the effect of APP on mononuclear phagocytes and acts by competitively inhibiting the access of FPRL1 to APP.

Molecular Function

Receptor antagonist activity; Apoptosis; Leukocyte chemotaxis

Subcellular Location

Secreted; Cytoplasm

Expression

Expressed in the heart, skeletal muscles, kidney and liver. Lesser but significant expression is observed in the brain and the gastrointestinal tract. Expressed in the AD brain, where it is found in some of the large intact neurons of the occipital lobes and small and round reactive glial cells in the hippocampus.

Structure

Largely unstructured in aqueous solution.

Alternative Nomenclature

HN antibody

HUNIN Antibody

MT-RNR2 antibody