General Information

Product

FOXP3 Antibody

Description

Forkhead box protein 3 Antibody

Verified Applications

ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Cat, Human, Monkey, Mouse, Rat

Predicted: Pig, Cynomolgus monkey, Rhesus monkey, Deer

Immunogen

Synthetic peptide corresponding to amino acids 229-250 of Mouse Forkhead Box Protein 3 (winged helix transcription factor 3 protein). The peptide was post synthetically modified to achieve desired antigenicity before coupling to a carrier protein.

Sequence:

AQCLLQREVVQSLEQQLELEKE

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.7 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyconal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:15,000-1:20,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9H5F2

Overview

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.

Molecular Function

Core promoter binding; Histone Acetyltransferase binding; Histone deacetylase binding; NFAT protein binding; NF-kappaB binding; Activator; Repressor

Subcellular Location

Nucleus; Cytoplasm

Expression

Down-regulated in regulatory T-cells (Treg) during inflammation.

Structure

Homodimer. Interacts with IKZF3. Isoform 1 (via LXXLL motif), but not isoform 2, interacts with isoform 4 of RORA (via AF-2 motif). Interacts with STUB1, HSPA8 and HSPA1A/B. Interacts with PPP1CA, PPP1CB and PPP1CG. Interacts with KAT5 and HDAC7. Interacts with HDAC9 in the absence of T-cell stimulation. Interacts with USP7. Interacts with isoform 2 of ZFP90 and can form a complex with TRIM28 in the presence of isoform 2 of ZFP90. Interacts with RUNX1. Interacts with RORC. Interacts with RELA and NFATC2. Interacts with RUNX2, RUNX3 and IKZF4.

Alternative Nomenclature

AIID antibody

DIETER antibody

Forkhead box P3 antibody

FOXP3delta7 antibody

Immune dysregulation polyendocrinopathy enteropathy X linked antibody

IPEX antibody

JM2 antibody

MGC141961 antibody

MGC141963 antibody

PIDX antibody

SCURFIN antibody

XPID antibody

General Information

Product

Phospho-AKT1 Antibody

Description

Affinity Purified Phosphorylated Rac protein kinase-alpha Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bird, Bovine, Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 430-480 on human Rac protein kinase-alpha protein.

Accession #

GenBank: AAA36539.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P31749

Overview

One of 3 serine/threonine-protein kinases (AKT1-AKT3) regulates many processes including metabolism, proliferation, cell survival, growth and angiogenesis.

Molecular Function

Developmental protein; Kinase; Serine/threonine-protein kinase; Transferase

Subcellular Location

Cytoplasm; Nucleus; Cell membrane

Expression

Expressed in prostate cancer and all human cell types.

Structure

Interacts with and phosphorylated by PDPK1.  Interacts with several proteins invovled in phosphorylation and ubiquitination

Alternative Nomenclature

AKT 1 antibody

C AKT antibody

MGC9965 antibody

MGC99656 antibody

Oncogene AKT1 antibody

PKB antibody

PKB-ALPHA antibody

PRKBA antibody

Protein Kinase B Alpha antibody

Protein kinase B antibody

Proto-oncogene c-Akt antibody

RAC Alpha antibody

RAC antibody

RAC PK Alpha antibody

RAC Serine/Threonine Protein Kinase antibody

RAC-PK-alpha antibody

vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody

General Information

Product

NEDD4 Antibody

Description

Affinity Purified E3 ubiquitin-protein ligase NEDD4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 840-880 on rat Nedd4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.6-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

Q62940

Overview

E3 ubiquitin-protein ligase;Involved in the ubiquitination of ebola virus VP40 protein and this ubiquitination plays a role in facilitating viral budding

Molecular Function

Ligase

Subcellular Location

Cell membrane, Cytoplasm, Membrane

Expression

Ubiquitously expressed. Expression is highest in lung, kidney and brain

Alternative Nomenclature

Cell proliferation-inducing gene 53 protein antibody

E3 ubiquitin protein ligase Nedd4 antibody

KIAA0093 antibody

NEDD 4 antibody

Neural precursor cell expressed developmentally down regulated 4 antibody

General Information

Product

VPRBP Antibody

Description

Affinity Purified VPR Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human Immunodeficiency Virus 1

Immunogen

Synthetic peptide taken within amino acid region 1-27 on human VPR protein.

Accession #

GenBank: AGV39791.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

T2D0A6

Overview

During virus entry, involved in the transport of the viral pre-integration (PIC) complex to the nucleus (PubMed:12417576). This function is crucial for viral infection of non-dividing macrophages (PubMed:12417576). May act directly at the nuclear pore complex, by binding nucleoporins phenylalanine-glycine (FG)-repeat regions. During virus replication, may deplete host UNG protein, and incude G2-M cell cycle arrest(PubMed:7666531, PubMed:7474100). Acts by targeting specific host proteins for degradation by the 26S proteasome, through association with the cellular CUL4A-DDB1 E3 ligase complex by direct interaction with host VPRPB/DCAF-1. Cell cycle arrest reportedly occurs within hours of infection and is not blocked by antiviral agents, suggesting that it is initiated by the Vpr carried into the virion. Additionally, Vpr induces apoptosis in a cell cycle dependent manner suggesting that these two effects are mechanistically linked. Detected in the serum and cerebrospinal fluid of AIDS patient, Vpr may also induce cell death to bystander cells (PubMed:11000244).

Molecular Function

Viral Life Cycle

Subcellular Location

Virion; Host nucleus; Host Extracellular Space

Structure

Homooligomer, may form homodimer (PubMed:7798208). Interacts with p6-gag region of the Pr55 Gag precursor protein through a (Leu-X-X)4 motif near the C terminus of the P6gag protein (PubMed:7474102). Interacts with host UNG (PubMed:9151883). May interact with host RAD23A/HHR23A (PubMed:9371639). Interacts with host VPRBP/DCAF1, leading to hijack the CUL4A-RBX1-DDB1-DCAF1/VPRBP complex, mediate ubiquitination of host proteins such as TERT and ZGPAT and arrest the cell cycle in G2 phase (PubMed:17314515, PubMed:17630831, PubMed:17314515).

Alternative Nomenclature

DCAF1 antibody

DDB1 and CUL4 associated factor 1 antibody

HIV-1 Vpr-binding protein antibody

KIAA0800 antibody

protein VPRBP antibody

RIP antibody

Vpr (HIV-1) binding protein antibody

VPR BP antibody

Vpr interacting protein antibody

Vpr-binding protein antibody

General Information

Product

PELO Antibody

Description

Affinity Purified PELOTA Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 330-380 on human PELOTA protein.

Accession #

GenBank: AAG22575.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q9BRX2

Overview

Is necessary in cell division for normal chromosome segregation and genomic stability.  It may recognize stalled ribosomes and trigger endonucleolytic cleavage of the mRNA, and may have ribonuclease activity.

Molecular Function

Endonuclease, Hydrolase, Nuclease

Subcellular Location

Nuclease, Cytoplasm

Expression

Expressed ubiquitously

Structure

The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity (Potential).  Belongs to the eukaryotic release factor 1 family. Pelota subfamily.

Alternative Nomenclature

CGI 17 antibody

pelo antibody

pelota (Drosophila) homolog antibody

pelota homolog antibody

PRO1770 antibody

Protein pelota homolog antibody

General Information

Product

UBR1 Antibody

Description

Affinity Purified E3 ubiquitin-protein ligase UBR1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1260-1500 on human UBR1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.68-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:4,000

ELISA

1:4,000

Immunohistochemistry

1:50-:150

Western Blot

1:500

Protein

Uniprot #

Q8IWV7

Overview

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. May be involved in pancreatic homeostasis. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth.

Molecular Function

Leucine binding; Ubiquitin-protein transferase activity; ligase activity; Zinc ion binding

Subcellular Location

Cytosol

Expression

Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).

Structure

The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.

The UBR-type zinc finger forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for Arginine in first position, has poor affinity for histidine, and doesn't bind acetylated peptides.

Alternative Nomenclature

E3 ubiquitin-protein ligase UBR1 antibody

JBS antibody

N-recognin-1 antibody

ubiquitin protein ligase E3 component n-recognin 1 antibody

Ubiquitin-protein ligase E3-alpha-1 antibody UBR1 antibody

General Information

Product

C15orf42 Antibody Affinity Purified

Description

Affinity Purified Treslin Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human Treslin protein.

Accession #

NCBI:  NP_689472.3

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q7Z2Z1

Overview

Regulates triggering of DNA replication onto replication origins, required for transition from pre-replication complex to pre-initiation complex. Required to prevent mitotic entry after treatment with ionizing radiation.

Molecular Function

Chromatin binding

Subcellular Location

Nucleus

Alternative Nomenclature

C15orf42 antibody

chromosome 15 open reading frame 42 antibody

replication-stimulating protein antibody

SLD3 antibody

SLD3 homolog antibody

SLD3, yeast , homolog of antibody

TICRR antibody

TopBP1-interacting checkpoint and replication regulator antibody

Treslin antibody

General Information

Product

Topoisomerase II alpha Antibody

Description

Affinity Purified DNA topoisomerase (ATP-hydrolysing) Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

Accession #

GenBank: CAA09762.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.54-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P11388

Overview

Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

Molecular Function

Isomerase, Topoisomerase, ATP binding

Subcellular Location

Cytoplasm, Nucleus

Structure

Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity.

Alternative Nomenclature

alpha isozyme antibody

ATP hydrolyzing DNA topoisomerase II alfa antibody

DNA gyrase antibody

DNA topoisomerase 2 alpha antibody

DNA topoisomerase II 170 kD antibody

DNA topoisomerase II alpha isozyme antibody

TOP2 antibody

TOP2A antibody

Topoisomerase DNA II alpha 170kDa antibody

TP2A antibody

Synthetic peptide taken within amino acid region 1220-1270 on human DNA topoisomerase (ATP-hydrolysing) protein.

General Information

Product

Cyclin D1 Antibody

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 1-50 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody

General Information

Product

Cyclin D1 Antibody Affinity Purified

Description

Affinity Purified G1/S-specific cyclin-D1 Antibody Mid-Region

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid regions 10-60 on human Cyclin D1 protein.

Accession #

GenBank: AAH23620.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65-0.70 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P24385

Overview

Regulatory component of the cyclin D1-CDK4 complex that phosphorylates and inhibits members of retinoblastoma protein family including RB1 and regulates the cell-cycle during G1/S transition.

Molecular Function

Cell cycle; Cell division; DNA damage; Transcription regulation

Subcellular Location

Cytoplasm; Membrane; Nucleus

Structure

Belongs to the Cyclin D subfamily.  Contains 1 cyclin N-terminal domain

Alternative Nomenclature

B cell CLL/lymphoma 1 antibody

CCND 1 antibody

Cyl 1 antibody

G1/S specific cyclin D1 antibody

Parathyroid adenomatosis 1 antibody

PRAD1 antibody

U21B31 antibody