General Information |
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Product |
Pan PDE4C Antibody FITC-Conjugated |
Description |
FITC-Conjugated cAMP-specific 3',5'-cyclic phosphodiesterase 4C Antibody |
Verified Applications |
CM, ELISA, ICC, IF, IHC, IP, WB |
Host |
Rabbit |
Species Cross Reactivity |
Human, Mouse, Rat |
Immunogen |
Synthetic cyclic carboxy-terminal peptide common to all PDE4C variants. |
Specificity | This antibody detects 3 isoforms of PDE4C subclasses: PDE4C1, PDE4C2, and PDE4C3. |
Accession # |
NCBI: NP_001074.2 |
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Quantity |
100 µg |
Volume |
200 µl |
Concentration |
0.65 µg/µl in antibody stabilization buffer |
Form |
FITC-Conjugated |
Clonality |
Polyclonal |
Storage |
-20⁰C for long term storage |
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|
Confocal Microscopy |
1:200 |
DB |
1:10,000 |
ELISA |
1:10,000 |
Immunocytochemistry |
1:200 |
Immunofluorescence |
1:200 |
Immunohistochemistry |
1:200 |
Immunoprecipitation |
1:200 |
Western Blot |
1:500-1:2,500 |
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Uniprot # |
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Overview |
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
Molecular Function |
Hydrolase, 3',5'-cyclic-AMP phosphodiesterase activity, cAMP binding |
Subcellular Location |
Cell projection > cillium |
Expression |
Expressed in various tissues but not in cells of the immune system. |
Structure |
Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2. |
Alternative Nomenclature |
5''-cyclic phosphodiesterase 4C antibody cAMP specific 3' 5' cyclic phosphodiesterase 4C antibody cAMP-specific 3'' antibody DPDE 1 antibody MGC126222 antibody PDE 21 antibody PDE 4C antibody Pde4c antibody Phosphodiesterase 4C antibody Phosphodiesterase E1 dunce homolog antibody Phosphodiesterase type 4C antibody |
Protein information supplied by UniProt
FabGennix PDE4C Antibody has been used in 15 Publications
Applications: Coimmunoprecipitation, Immunocytochemistry, Immunohistochemistry, and Western blot
Reactivity: Human, Mouse, and Rat
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Cheng, Hao, et al. "siRNA‑mediated silencing of phosphodiesterase 4B expression affects the production of cytokines in endotoxin‑stimulated primary cultured microglia." Experimental and Therapeutic Medicine 12.4 (2016).
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PMID: 27698721 Applications: Western Blot Reactivity: Rat |
Ji, Qing, et al. "Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation." Molecular medicine reports 13.2 (2016).
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PMCID: 26706904 Applications: Western Blot Reactivity: Rat |
Massimi, Mara, et al. "Increase of Intracellular Cyclic AMP by PDE4 Inhibitors Affects HepG2 Cell Cycle Progression and Survival." Journal of Cellular Biochemistry (2016).
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PMCID: 27859531 Applications: Western Blot Reactivity: Human |
Petersen, T. S., et al. "Distribution and function of 3', 5'-Cyclic-AMP phosphodiesterases in the human ovary." Molecular and cellular endocrinology (2015).
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PMID: 25578602 Applications: Immunohistochemistry Reactivity: Human |
Yougbaré, Issaka, et al. "NCS 613 Exhibits Anti-Inflammatory Effects On Pbmcs From Lupus Patients By Inhibiting P38 MAPK And NF-Κb Signalling Pathways While Reducing Proinflammatory Cytokine Production." Canadian Journal Of Physiology & Pharmacology 91.5 (2013).
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PMID: 23656347 Applications: Immunohistochemistry, Western Blot Reactivity: Human |
Haque, Mohammed Z., Paulo S. Caceres, and Pablo A. Ortiz. “β-Adrenergic Receptor Stimulation Increases Surface NKCC2 Expression in Rat Thick Ascending Limbs in a Process Inhibited by Phosphodiesterase 4.” American Journal of Physiology - Renal Physiology 303.9 (2012).
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PMCID: 3518189 Applications: Western Blot Reactivity: Rat |
Keravis, Thérèse et al. “Disease Progression in MRL/lpr Lupus-Prone Mice Is Reduced by NCS 613, a Specific Cyclic Nucleotide Phosphodiesterase Type 4 (PDE4) Inhibitor.” Ed. Pierre Bobé. PLoS ONE 7.1 (2012).
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PMCID: 3256138 Applications: Western Blot Reactivity: Mouse |
Abusnina, Abdurazzag, et al. "Down-regulation of cyclic nucleotide phosphodiesterase PDE1A is the key event of p73 and UHRF1 deregulation in thymoquinone-induced acute lymphoblastic leukemia cell apoptosis." Cellular signalling 23.1 (2011).
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PMID: 20807569 Applications: Western Blot Reactivity: Human |
Yougbare, Issaka, et al. "NCS 613, a potent and specific PDE4 inhibitor, displays anti-inflammatory effects on Human lung tissues." American Journal of Physiology-Lung Cellular and Molecular Physiology 301.4 (2011).
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PMID: 21784969 Applications: Immunocytochemistry, Western Blot Reactivity: Human |
Tahseldar-Roumieh, Rima et al. “PDEs1-5 Activity and Expression in Tissues of Cirrhotic Rats Reveal a Role for Aortic PDE3 in NO Desensitization.” International Journal of Experimental Pathology 90.6 (2009).
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PMID: 2803251 Applications: Western Blot Reactivity: Rat |
Whitaker, Christopher M., and Nigel G. F. Cooper. “The Novel Distribution of Phosphodiesterase-4 Subtypes Within the Rat Retina.” Neuroscience 163.4 (2009).
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PMCID: 2774823 Applications: Western Blot Reactivity: Rat |
Campos-Toimil, M et al. “Short-Term or Long-Term Treatments with a Phosphodiesterase-4 (PDE4) Inhibitor Result in Opposing Agonist-Induced Ca2+ Responses in Endothelial Cells.” British Journal of Pharmacology 154.1 (2008).
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PMCID: 2438981 Applications: Western Blot Reactivity: Human |
Sachs, Benjamin D. et al. “P75 Neurotrophin Receptor Regulates Tissue Fibrosis through Inhibition of Plasminogen Activation via a PDE4/cAMP/PKA Pathway.” The Journal of Cell Biology 177.6 (2007). |
PMCID: 2064370 Applications: Coimmunoprecipitation, Western Blot Reactivity: Mouse |
Murray, Fiona, et al. "Expression and activity of cAMP phosphodiesterase isoforms in pulmonary artery smooth muscle cells from patients with pulmonary hypertension: role for PDE1." American Journal of Physiology-Lung Cellular and Molecular Physiology 292.1 (2007).
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PMID: 16980375 Applications: Western Blot Reactivity: Human |
Gallagher, Helen C., et al. "Valproate activates phosphodiesterase-mediated cAMP degradation: relevance to C6 glioma G1 phase progression." Neurotoxicology and teratology 26.1 (2004).
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PMID: 15001216 Applications: Western Blot Reactivity: Human |
Publishing research using PD4C-301AP? Please let us know, we can cite the reference in this datasheet.
Product | Note | Status | Price | |
---|---|---|---|---|
|
PD4C-301AP | $320.00 * | ||
|
P-PD4C | $155.00 * | ||
|
PD4C-BIOTIN | $375.00 * | ||
|
PC-PD4C | $220.00 * | ||
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