General Information

Product

Eprhin Receptor B4 Antibody

Description

Affinity Purified Ephrin type-B receptor 4 precursor Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human

Immunogen

Synthetic peptide taken within amino acid region 900-984 on human Ephrin B4 receptor protein.

Accession #

NCBI:  NP_004435

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.62 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P54760

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell Membrane

Expression

Abundantly expressed in placenta but also detected in kidney, liver, lung, pancreas, skeletal muscle and heart. Expressed in primitive and myeloid, but not lymphoid, hematopoietic cells. Also observed in cell lines derived from liver, breast, colon, lung, melanocyte and cervix.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses.

Alternative Nomenclature

Ephb4 antibody

Ephrin type-B receptor 4 antibody

Hepatoma Transmembrane Kinase antibody

HTK antibody

MYK 1 antibody

MYK1 antibody

TYRO 11 antibody

Tyrosine-protein kinase receptor HTK antibody

Tyrosine-protein kinase TYRO11 antibody

General Information

Product

Eprhin Receptor B3 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-B receptor 3 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 100-200 on EphBR3 protein.

Accession #

NCBI:  NP_034273

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

P54754

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Dendrite

Expression

Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk. Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level).

Structure

Contains 1 Eph LBD domain; Contains 2 fibronectin type-III domains; Contains 1 protein kinase domain; Contains 1 SAM domain

Alternative Nomenclature

Cek10 antibody

EK2 antibody

Embryonic kinase 2 antibody

EPH Like Tyrosine Kinase 2 antibody

EPH receptor B3 antibody

EPH-like kinase 2 antibody

ephb3 antibody

Ephrin type-B receptor 3 antibody

ETK2 antibody

hEK2 antibody

Human Embryo Kinase 2 antibody

Mdk5 antibody

Sek4 antibody

TYRO6 antibody

Tyrosine protein kinase receptor HEK2 antibody

Tyrosine-protein kinase TYRO6 antibody

General Information

Product

Ephrin Receptor A3 Antibody

Description

Affinity Purified Ephrin Type-A Receptor 3 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 900-980 on human EPHA3 protein

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P29320

Overview

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.

Molecular Function

ATP binding; Nucleotide binding

Subcellular Location

Isoform 1:  Cell membrane; Single-pass type I membrane protein.  Isoform 2:  Secreted

Expression

Widely expressed. Highest level in placenta.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Forms a ternary EFNA5-EPHA3-ADAM10 complex mediating EFNA5 extracellular domain shedding.

Alternative Nomenclature

AW492086 antibody

Cek4 antibody

EC 2.7.10.1 antibody

EK4 antibody

End3 antibody

Eph receptor A3 antibody

EPH-like kinase 4 antibody

EPH-like tyrosine kinase 1 antibody

EphA3 antibody

Ephrin type-A receptor 3 antibody

ETK 1 antibody

ETK antibody

HEK 4 antibody

HEK antibody

Human embryo kinase 1 antibody

Mek4 antibody

MGC109882 antibody

Receptor tyrosine kinase HEK antibody

Tyro 4 antibody

Tyrosine-protein kinase TYRO4 antibody

General Information

Product

CRMP1 Antibody

Description

Affinity Purified Collapsin response mediated protein 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to 80-130 on CRMP1 protein.

Accession #

GenBank: EDL37524.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.53-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

UPI0001548EB3

Overview

Hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides

Molecular Function

Neuron Development; Pyrimidine nucleobase catabolic process

Subcellular Location

Cytoplasm; Dendrite; Neuronal cell body

Alternative Nomenclature

Collapsin response mediator protein 1 antibody

CRMP 1 antibody

Dihydropyrimidinase like 1 antibody

Dihydropyrimidinase related protein 1 antibody

DPYSL1 antibody

DRP 1 antibody

ULIP-3 antibody

Unc-33-like phosphoprotein 3 antibody

General Information

Product

DRP1 Antibody

Description

Dynamin-related Protein 1 Antibody

Verified Applications

ELISA, ICC, IF, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

D. Melanogaster, Human, Mouse, Rat

Immunogen

Synthetic peptide within 450-490 on human DRP1 protein.

Accession #

GenBank: BAC06576.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.68 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Clonality

Polyclonal

Storage

-20°C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

Western Blot

1:450

Protein

Uniprot #

Q8K1M6

Overview

Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism.

Molecular Function

GTP-binding, Lipid-binding, Nucleotide-binding

Subcellular Location

Golgi apparatus; Peripheral membrane protein; peroxisome; Mitochondrion outer membrane; Clathrin-coated pit; synaptic vesicle membrane

Expression

Expressed in the cerebellum and in several regions of the cerebrum and diencephalon

Structure

Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Oligomerizes in a GTP-dependent manner to form membrane-associated tubules with a spiral pattern. Can also oligomerize to form multimeric ring-like structures.

Alternative Nomenclature

DLP1 antibody

dnm1l antibody

Dnm1p/Vps1p-like protein antibody

dnml1 antibody

DRP1 antibody

DVLP antibody

Dymple antibody

Dynamin 1 like antibody

Dynamin family member proline-rich carboxyl-terminal domain less antibody

Dynamin like protein antibody

Dynamin-like protein 4 antibody

Dynamin-related protein 1 antibody

DYNIV 11 antibody

EMPF antibody

FLJ41912 antibody

HdynIV antibody

VPS1 antibody

General Information

Product

GIRK2 Antibody Affinity Purified

Description

Affinity Purified G protein-activated inward rectifier potassium channel 2 Antibody

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid 1-50 on human GIRK2 protein.

Accession #

GenBank: Q8QZW1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.60-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q8QZW1

Overview

G-protein activated inward rectifier potassium channel activity.

Molecular Function

Potassium

Subcellular Location

Membrane; Multi-pass membrane protein

Structure

Belongs to inward rectifier-type potassium channel family

Alternative Nomenclature

BIR1 antibody

G protein-activated inward rectifier potassium channel 2 antibody

hiGIRK2 antibody

Inward rectifier K(+) channel Kir3.2 antibody

Inward rectifier potassium channel KIR3.2 antibody

inwardly rectifying subfamily J member 6 antibody

KATP-2 antibody

Kcnj6 antibody

Kcnj7 antibody

KIR3.2 antibody

Potassium channel antibody

General Information

Product

LAMP1 Antibody

Description

Affinity Purified Lysosome-associated membrane glycoprotein 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey

Immunogen

Synthetic peptide taken within amino acid region 300-350 on human LAMP1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P11279

Overview

Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.

Molecular Function

Enzyme binding

Subcellular Location

Cell membrane, single-pass type I membrane protein, endosome membrane, lysosome membrane (Note:This protein shuttles between lysosomes, endosomes, and plasma membrane. Colocalizes with OSBPL1A at the late endosome)

Alternative Nomenclature

CD107 antigen like family member A antibody

LAMP 1 antibody

LAMPA antibody

LGP120 antibody

lgpA antibody

Lysosomal Associated Membrane Protein 1 antibody

General Information

Product

LIMK1 Antibody

Description

Affinity Purified LIM domain kinase 1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Chicken

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.

Accession #

NCBI:  NP_002305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53667

Overview

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.

Molecular Function

ATP binding, protein kinase activity, protein serine/threonine kinase activity, zinc ion binding, heat shock protein binding

Subcellular Location

Cytoplasm, nucleus

Expression

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Alternative Nomenclature

LIM domain kinase 1 antibody

LIM motif-containing protein kinase antibody

LIMK antibody

LIMK-1 antibody

General Information

Product

MSX1 Antibody 

Description

Affinity Purified MSX1 protein Antibody

Verified Applications

DB, ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide:

DEEGAKPKVSPSLLPFSVE

corresponding to amino acids 46-64 of Mouse Msx1 protein. The peptide was post synthetically modified and conjugated to KLH using hetero bifunctional cross linker for immunization of rabbits. 

Accession #

GenBank: AAH67353.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:20,000-1:50,000

ELISA

1:20,000-1:50,000

Western Blot

1:500-1:750

Protein

Uniprot #

P28360

Overview

Acts as a transcriptional repressor;play a role in limb-pattern formation,cranofacial development and odontogenesis

Molecular Function

Developmental protein, Repressor

Subcellular Location

Nucleus

Expression

Expressed in the developing nail bed mesenchyme.

Structure

Belongs to the Msh homeobox family

Alternative Nomenclature

AA675338 antibody

AI324650 antibody

Homeobox protein Hox-7 antibody

Homeobox protein MSX 1 antibody

HOX 7 antibody

HYD 1 antibody

msh (Drosophila) homeo box homolog 1

Msh antibody

MSX 1 antibody

OFC5 antibody

STHAG1 antibody

General Information

Product

Phospho Survivin Antibody Affinity Purified

Description

Affinity Purified Phosphorylated Baculoviral IAP repeat-containing protein 5 isoform 2 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Phosphorylated synthetic peptide corresponding to unique amino acid sequences on human Survivin protein.

Accession #

NCBI:  NP_001012270.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50-1.50 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O15392

Overview

Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.

Molecular Function

Protease inhibitor, Repressor, Thiol protease inhibitor

Subcellular Location

Cytoplasm, Nucleus, Chromsome, Centromere, Cytoskeleton, Spindle, Kinetochore, Midbody

Expression

Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.

Structure

Monomer or Homodimer.

Alternative Nomenclature

API4 antibody

Apoptosis Inhibitor 4 antibody

Apoptosis inhibitor survivin antibody

Baculoviral IAP repeat-containing protein 5 antibody

BIRC5 antibody

EPR 1 antibody

IAP4 antibody

Survivin variant 3 alpha antibody

SVV antibody

TIAP antibody