General Information

Product

LIMK1 Antibody

Description

Affinity Purified LIM domain kinase 1 isoform 1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.

Accession #

NCBI:  NP_002305.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P53667

Overview

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.

Molecular Function

ATP binding, protein kinase activity, protein serine/threonine kinase activity, zinc ion binding, heat shock protein binding

Subcellular Location

Cytoplasm, nucleus

Expression

Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Alternative Nomenclature

EC 2.7.11.1 antibody

LIM domain kinase 1 antibody

LIM motif-containing protein kinase antibody

LIMK antibody

LIMK-1 antibody

General Information

Product

Pax2 Antibody

Description

Affinity Purified Pan Paired box protein Pax-2 Antibody Mid-region

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 255-305 on human PAX2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-region

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250-1:500

Protein

Uniprot #

Q02962

Overview

Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS.

Molecular Function

Developmental protein, core promoter proximal region sequence-specific DNA binding, DNA binding

Subcellular Location

Nucleus

Expression

Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.

Structure

Mainly in fetal kidney and juvenile nephrogenic rests.

Alternative Nomenclature

Paired box 2 antibody

Paired box gene 2 antibody

paired box protein 2 antibody

Paired box protein Pax 2 antibody

Pax 2 antibody

General Information

Product

PAX2 isoform d + e Antibody

Description

Affinity Purified Paired box protein Pax-2 isoform d and Pax-2 isoform e Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 367-417 on human PAX2 isoform d protein.

Specificity

This antibody will detect Pax-2 isoform d and Pax-2 isoform e. Will not detect other Pax-2 isoforms.

Accession #

NCBI:  NP_003980.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.75-0.95 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q02962

Overview

Probable transcription factor that may have a role in kidney cell differentiation. Has a critical role in the development of the urogenital tract, the eyes, and the CNS.

Molecular Function

Developmental protein, core promoter proximal region sequence-specific DNA binding, DNA binding

Subcellular Location

Nucleus

Expression

Expressed in primitive cells of the kidney, ureter, eye, ear and central nervous system.

Structure

Mainly in fetal kidney and juvenile nephrogenic rests.

Alternative Nomenclature

Paired box 2 antibody

Paired box gene 2 antibody

paired box protein 2 antibody

Paired box protein Pax 2 antibody

Pax 2 antibody

General Information

Product

Lamin B1 antibody - Nuclear Envelope Marker Antibody

Description

Affinity Purified B1 antibody - Nuclear Envelope Marker Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 450-500 on human Lamin-B1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P20700

Overview

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Molecular Function

phospholipase binding

structural molecule activity

Subcellular Location

Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side

Expression

Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12.

Structure

Belongs to the intermediate filament family.

Alternative Nomenclature

ADLD antibody

lamin B1 antibody

LMN antibody

LMN2 antibody

LMNB antibody

Lmnb1 antibody