General Information

Product

RASD2 Antibody

Description

Affinity Purified Ras homolog enriched in striatum Antibody

Verified Applications

ELISA, IHC ,IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-317 on RASD2 protein.

Accession #

GenBank: AAH36988.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Dot Blot

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:500

Western Blot

1:500-1:1,000

Protein

Uniprot #

Q8JZW1

Molecular Function

GTP binding

Alternative Nomenclature

GTP binding protein Rhes antibody

MGC:4834 antibody

Ras homolog enriched in striatum antibody

RASD 2 antibody

Rhes antibody

TEM2 antibody

Tumor endothelial marker 2 antibody

General Information

Product

RPE65 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on mouse RPE65 protein.

Accession #

GenBank: AAL01119.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.51-0.63 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Western Blot

1:500

Protein

Uniprot #

Q91ZQ5

Overview

Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester.

Molecular Function

Hydrolase, Isomerase

Subcellular Location

Cytoplasm, Cell membrane; Lipid anchor, Microsome membrane

Expression

Retinal pigment epithelium  specific

Structure

Interacts with MYO7A; this mediates light-dependent intracellular transport of RPE65.

Alternative Nomenclature

All-trans-retinyl-palmitate hydrolase antibody

LCA 2 antibody

Leber congenital amaurosis antibody

p63 antibody

rd 12 antibody

Retinal pigment epithelium specific 61 kDa protein antibody

Retinal pigment epithelium specific 65 kDa protein antibody

retinitis pigmentosa 20 antibody

Retinoid isomerohydrolase antibody

RP20 antibody

General Information

Product

Synaptoagmin 1 Antibody Affinity Purified

Description

Affinity Purified SYT1 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic  peptide taken within amino acid region 1-60 on SYT1 protein.

Accession #

GenBank: AAH58917.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P21579

Overview

May have regulatory rol in membrane interactions during trafficking of synaptic vesicles at active zones of synapse. Binds acidic phospholipids, can bind to at least three additional proteins in a CA2+ independent manner.

Molecular Function

1-phosphatidylinositol binding, calcium dependent phospholipid binding, calcium ion binding, low-density lipoprotein particle receptor binding, phosphatidylserine binding, transporter activity

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, single-pass membrane protein (Note: Synaptic vesicles and chromaffin granules.)

Alternative Nomenclature

P65 antibody

SVP65 antibody

Synaptotagmin 2 antibody

Synaptotagmin 1 antibody

SYT antibody

SYT1 antibody

Syt2 antibody

SytI antibody

General Information

Product

Eprhin Receptor A5 Antibody Affinity Purified

Description

Affinity Purified Ephrin Type-1 Receptor isoform a Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 600-700 on human EphA R5 protein.

Accession #

NCBI:  NP_004430

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

P54756

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity).

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

Alternative Nomenclature

Brain-specific kinase antibody

bsk antibody

CEK 7 antibody

Eck like sequence 1 antibody

EHK 1 antibody

EK7 antibody

Els 1 antibody

Eph homology kinase 1 antibody

EPH-like kinase 7 antibody

EphA5 antibody

Ephrin type-A receptor 5 antibody

HEK 7 antibody

Rek 7 antibody

TYRO 4 antibody

Tyrosine protein kinase receptor EHK 1 antibody

General Information

Product

Eprhin Receptor A6 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-A receptor 6 isoform A Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 800-900 on human EphA R6 protein.

Accession #

NCBI:  NP_001073917

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

B3KS12

Overview

Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity).

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein

Expression

Expressed in brain and testis.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).

Alternative Nomenclature

Ehk 2 antibody

EPH homology kinase 2 antibody

Eph Receptor A6 antibody

ephrin receptor EphA6 antibody

Hek12 antibody

General Information

Product

Eprhin Receptor A8 Antibody Affinity Purified

Description

Affinity Purified Ephrin Type-A receptor 8 isoform 1 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequences on human EphA R8 protein.

Accession #

NCBI:  NP_065387

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane; Single-pass type I membrane protein; Early endosome membrane

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. May also form heterodimers with other ephrin receptors (By similarity).

Alternative Nomenclature

2.7.1.112 antibody

EEK antibody

EK3 antibody

EPH and ELK related kinase antibody

EPH like kinase 3 antibody

EphA8 antibody

Ephrin type-A receptor 8 antibody

HEK3 antibody

KIAA1459 antibody

Tyrosine-protein kinase receptor EEK antibody

Tyrosylprotein Kinase antibody

General Information

Product

Eprhin Receptor A4 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-A receptor 4 Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 550-650 on human EphA R4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q03137

Overview

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein

Expression

Highest expression in the adult brain and retina and also detectable in kidney, lung, skeletal muscle and thymus. Not detected in heart and liver.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts (via

Alternative Nomenclature

Cek 8 antibody

EK8 antibody

eph receptor a4 antibody

EPH-like kinase 8 antibody

EphA4 antibody

Ephrin receptor Eph A4 antibody

hEK8 antibody

Receptor protein tyrosine kinase HEK 8 antibody

Sek 1 antibody

SEK antibody

TYRO 1 protein tyrosine kinase antibody

Tyrosine-protein kinase receptor SEK antibody

General Information

Product

Eprhin Receptor B2 Antibody Affinity Purified

Description

Affinity Purified Ephrin type-B receptor 2 isoform 2 precursor Antibody

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-150 on mouse Ephrin BR2 protein.

Accession #

NCBI:  NP_034272

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58-0.61 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

A3KG01

Overview

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Membrane

Structure

Contains sterile alpha motif domain; Conains protein kinase domain

Alternative Nomenclature

Cek 6 antibody

EK6 antibody

ELK antibody

Elkh antibody

Eph tyrosine kinase 2 antibody

EPH-like kinase 6 antibody

EPH2 antibody

EphB1 antibody

Ephrin receptor Eph B1 precursor antibody

Ephrin type B receptor 1 antibody

EPHT2 antibody

HEK 6 antibody

NET antibody

Neuronally expressed EPH related tyrosine kinase antibody

Tyrosine-protein kinase receptor EPH-2 antibody

General Information

Product

Eprhin Receptor B1 Antibody

Description

Affinity Purified Ephrin type-B receptor 1 isoform 1 Antibody

Verified Applications

ELISA, IHC, IP ,WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 850-900 on mouse Eph BR1 protein.

Accession #

NCBI:  NP_775623

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:750

Protein

Uniprot #

Q8CBF3

Overview

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.

Molecular Function

ATP-binding, Nucleotide-binding

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Dendrite

Expression

Expressed in neural stem and progenitor cells in the dentate gyrus.

Structure

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1. Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion. Then ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1.

Alternative Nomenclature

Cek 6 antibody

EK6 antibody

ELK antibody

Elkh antibody

Eph tyrosine kinase 2 antibody

EPH-like kinase 6 antibody

EPH2 antibody

EphB1 antibody

Ephrin receptor Eph B1 precursor antibody

Ephrin type B receptor 1 antibody

EPHT2 antibody

HEK 6 antibody

NET antibody

Neuronally expressed EPH related tyrosine kinase antibody

Tyrosine-protein kinase receptor EPH-2 antibody

General Information

Product

GFAP Antibody

Description

Affinity Purified Glial fibrillary acidic protein Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 50-100 on human glial fibrillary acidic protein.

Accession #

PRF:  226236

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P14136

Overview

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Molecular Function

Structural Constituent of Cytoskeleton; Astrocyte development; Bergmann glial cell differentiation

Subcellular Location

Cytoplasm

Expression

Expressed in cells lacking fibronectin.

Structure

Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).

Alternative Nomenclature

Astrocyte antibody

FLJ42474 antibody

FLJ45472 antibody

GFAP antibody

Glial Fibrillary Acidic Protein antibody

Intermediate filament protein antibody