General Information

Product

CDKN2A / p14ARF Antibody

Description

Affinity Purified partial tumor supressor p14ARF Antibody N-epitope

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Immunogen

N-epitope synthetic peptide corresponding to unique amino acid sequence on p14ARF protein

Accession #

GenBank: AAC6069.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

1.00-1.25 mg/ml µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q8N726

Overview

Functions as a tumor supressor and has the ability to induce cell cycle arrest in the G1 and G2 phases.  Serves to induce G2 arrest and apoptosis in a p53-independent manner via preventing the activation of cyclin B1/CDC2 complexes.

Molecular Function

DNA binding, RNA binding, p53 binding, MDM2/MDM4 family protein binding

Subcellular Location

Nucleus

Structure

No interaction with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6.  Interacts with TBRG1, COMMD1, CDKN2AIP and E4F1.  Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9.

Alternative Nomenclature

ARF antibody

CDK4 inhibitor p16 INK4 antibody

CDK4I antibody

CDKN 2A antibody

Cell cycle negative regulator beta antibody

CMM2 antibody

Cyclin dependent kinase 4 inhibitor A antibody

1/2/3 antibody

Cyclin dependent kinase inhibitor p16 antibody

INK4 antibody

Melanoma p16 inhibits CDK4 antibody

MLM antibody

MTS 1 antibody

p14 antibody

p16 antibody

p19 antibody

P19ARF antibody

TP16 antibody

General Information

Product

E2F1 Antibody Affinity Purified

Description

Affinity Purified E2F transcription factor 1 Antibody Mid-Region

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse Rat

Immunogen

Synthetic peptide taken within amino acid region 125-165 on E2F1 protein.

Accession #

NCBI:  NM_005225.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

Mid-Region

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:1,000

Protein

Uniprot #

Q01094

Overview

Transcription activator that binds DNA cooperatively with DP proteins through E2 recognition site

Molecular Function

DNA-binding; Apoptosis

Subcellular Location

Nucleus

Structure

Component of DRTF1/E2F transcription factor complex.  Forms heterodimers with DP family members.

Alternative Nomenclature

Dmel\CG6376 antibody

drosE2F1 antibody

E(Sev-CycE)3A antibody

E(var)3-93E antibody

Evar(3)164 antibody

l(3)07172 antibody

l(3)j3B1 antibody

l(3)j3C2 antibody

l(3)rM729 antibody

PBR 3 antibody

PRB binding protein E2F 1 antibody

RBAP 1 antibody

RBP 3 antibody

Retinoblastoma associated protein 1 antibody

Retinoblastoma binding protein 3 antibody

Transcription factor E2F1 antibody

General Information

Product

E2F1 Antibody Affinity Purified

Description

Affinity Purified E2F transcription factor 1 Antibody C-epitope

Verified Applications

ELISA, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse Rat

Immunogen

Synthetic peptide taken within amino acid region 380-430 on E2F1 protein.

Accession #

NCBI:  NM_005225.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:1,000

Protein

Uniprot #

Q01094

Overview

Transcription activator that binds DNA cooperatively with DP proteins through E2 recognition site

Molecular Function

DNA-binding; Apoptosis

Subcellular Location

Nucleus

Structure

Component of DRTF1/E2F transcription factor complex.  Forms heterodimers with DP family members.

Alternative Nomenclature

Dmel\CG6376 antibody

drosE2F1 antibody

E(Sev-CycE)3A antibody

E(var)3-93E antibody

Evar(3)164 antibody

l(3)07172 antibody

l(3)j3B1 antibody

l(3)j3C2 antibody

l(3)rM729 antibody

PBR 3 antibody

PRB binding protein E2F 1 antibody

RBAP 1 antibody

RBP 3 antibody

Retinoblastoma associated protein 1 antibody

Retinoblastoma binding protein 3 antibody

Transcription factor E2F1 antibody

General Information

Product

E2F1 Antibody

Description

Affinity Purified E2F transcription factor 1 Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse Rat

Immunogen

Synthetic peptide corresponding to amino acids 58-93 of Human E2F1.

Sequence:

PCDPDLLLFATPQAPRPTPSAPRPALGRPPVKRRLD

Accession #

NCBI: NM_005225.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.65 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:1,000

Protein

Uniprot #

Q01094

Overview

Transcription activator that binds DNA cooperatively with DP proteins through E2 recognition site.

Molecular Function

DNA-binding; Apoptosis

Subcellular Location

Nucleus

Structure

Component of DRTF1/E2F transcription factor complex.  Forms heterodimers with DP family members.

Alternative Nomenclature

Dmel\CG6376 antibody

drosE2F1 antibody

E(Sev-CycE)3A antibody

E(var)3-93E antibody

Evar(3)164 antibody

l(3)07172 antibody

l(3)j3B1 antibody

l(3)j3C2 antibody

l(3)rM729 antibody

PBR 3 antibody

PRB binding protein E2F 1 antibody

RBAP 1 antibody

RBP 3 antibody

Retinoblastoma associated protein 1 antibody

Retinoblastoma binding protein 3 antibody

Transcription factor E2F1 antibody

General Information

Product

ABL1 Antibody

Description

Affinity Purified Tyrosine-protein kinase ABL1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 990-1130 of human Isoform IB of Tyorsine-protein kinase ABL1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P00519

Overview

Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation); ANXA1 (involved in membrane anchoring); DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling); or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration.

Molecular Function

Kinase, Transferase, Tyrosine-protein kinase

Subcellular Location

Cytoplasm > cytoskeleton, Nucleus, Mitochondrion

Expression

Widely expressed.

Structure

Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily. Contains 1 proteins kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Alternative Nomenclature

Abelson murine leukemia viral oncogene homolog 1 antibody
Abelson tyrosine protein kinase 1 antibody
Abl 1 antibody
ABL antibody
ABL proto oncogene 1 non receptor tyrosine kinase antibody
ABL1 antibody
bcr/abl antibody
c abl oncogene 1 receptor tyrosine kinase antibodyJTK7 antibody
p150 antibody
Proto oncogene tyrosine protein kinase ABL1 antibody
Proto-oncogene c-Abl antibody
Tyrosine-protein kinase ABL1 antibody
v abl Abelson murine leukemia viral oncogene homolog 1 antibody
v abl antibody

General Information

Product

ATG16L1 Antibody

Description

Affinity Purified Autophagy-related protein 16-1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Predicted: Human

Immunogen

Synthetic peptide taken within amino acid region 560-610 on human Autophagy-related protein 16-1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q676U5

Overview

Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane.

Molecular Function

Identical protein binding, Ubiquitin-like protein transferase activity

Subcellular Location

Cytoplasm, Preautophagosomal structure membrane, Peripheral membrane protein

Structure

Homooligomer. Interacts with WIPI2, FIP200 and ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Interacts with RAB33B. Interacts with TMEM

Alternative Nomenclature

APG16 like 1 antibody

APG16L antibody

APG16L beta antibody

ATG16 autophagy related 16 like 1 antibody

ATG16A antibody

ATG16L antibody

Autophagy related protein 16 1 antibody

IBD10 antibody

WD repeat domain 30 antibody

WDR30 antibody

General Information

Product

Von Hippel Lindau Antibody

Description

Affinity Purified Von Hippel-Lindau disease tumor suppressor Antibody

Verified Applications

ELISA, IHC, IP, WB

Immunogen

Synthetic peptide taken within amino acid region 130-200 on human Von Hippel-Lindau disease tumor suppressor protein.

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Rat

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunocytochemistry

1:50-1:150

Immunohistochemistry

1:50-1:150

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

P40337

Overview

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.

Molecular Function

Enzyme binding, Transcription factor binding, Ubiquitin protein ligase activity,

Subcellular Location

Cytoplasm, Peripheral membrane protein, nucleus

Expression

Expressed in the adult and fetal brain and kidney.

Structure

Belongs to the VHL family.

Alternative Nomenclature

Elongin binding protein antibody

G7 protein antibody

HRCA 1 antibody

Protein G7 antibody

pVHL antibody

RCA 1 antibody

VHL 1 antibody

VHLH antibody

Von Hippel Lindau disease tumor suppressor antibody

von Hippel Lindau syndrome antibody

von Hippel Lindau tumor suppressor antibody

Von Hippel Lindau tumor suppressor, E3 ubiquitin protein ligase antibody

General Information

Product

Profilin Antibody

Description

Affinity Purified Profilin 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to amino acids within region 50-100 on human Profilin 1.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20?C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:500-1:5,000

Protein

Uniprot #

P07737

Overview

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androgen receptor (AR) and HTT aggregation and binding of G-actin is essential for its inhibition of AR.

Molecular Function

actin binding

actin monomer binding

adenyl-nucleotide exchange factor activity

cadherin binding

phosphatidylinositol-4,5-bisphosphate binding

phosphotyrosine residue binding

proline-rich region binding

RNA binding

Subcellular Location

cytoskeleton

Expression

Expressed in epididymis (at protein level).

Alternative Nomenclature

Actin binding protein antibody

ALS18 antibody

Epididymis tissue protein Li 184a antibody

OTTHUMP00000125244 antibody

PFN 1 antibody

Pfn antibody

PFN1 antibody

PROF1_HUMAN antibody

Profilin I antibody

Profilin1 antibody