General Information

Product

PKC eta Antibody Affinity Purified

Description

Affinity Purified Protein kinase C eta type Antibody C-epitope

Verified Applications

ELISA, IMM, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 500-550 on human Protein Kinase C eta type protein.

Accession #

NCBI:  NP_006246

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

P24723

Overview

Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in the regulation of cell differentiation in keratinocytes and pre-B cell receptor, mediates regulation of epithelial tight junction integrity and foam cell formation, and is required for glioblastoma proliferation and apoptosis prevention in MCF-7 cells. In keratinocytes, binds and activates the tyrosine kinase FYN, which in turn blocks epidermal growth factor receptor (EGFR) signaling and leads to keratinocyte growth arrest and differentiation. Associates with the cyclin CCNE1-CDK2-CDKN1B complex and inhibits CDK2 kinase activity, leading to RB1 dephosphorylation and thereby G1 arrest in keratinocytes. In association with RALA activates actin depolymerization, which is necessary for keratinocyte differentiation. In the pre-B cell receptor signaling, functions downstream of BLNK by up-regulating IRF4, which in turn activates L chain gene rearrangement. Regulates epithelial tight junctions (TJs) by phosphorylating occludin (OCLN) on threonine residues, which is necessary for the assembly and maintenance of TJs. In association with PLD2 and via TLR4 signaling, is involved in lipopolysaccharide (LPS)-induced RGS2 down-regulation and foam cell formation. Upon PMA stimulation, mediates glioblastoma cell proliferation by activating the mTOR pathway, the PI3K/AKT pathway and the ERK1-dependent phosphorylation of ELK1. Involved in the protection of glioblastoma cells from irradiation-induced apoptosis by preventing caspase-9 activation. In camptothecin-treated MCF-7 cells, regulates NF-kappa-B upstream signaling by activating IKBKB, and confers protection against DNA damage-induced apoptosis.

Molecular Function

ATP binding; Metal ion binding; Protein kinase C activity

Subcellular Location

Cytoplasm

Expression

Most abundant in lung, less in heart and skin.

Structure

The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.  Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Alternative Nomenclature

KPCL_HUMAN antibody

MGC 5363 antibody

MGC26269 antibody

MGC5363 antibody

nPKC eta antibody

PKC h antibody

PKC L antibody

PRKCH antibody

PRKCL antibody

Protein kinase C eta antibody

General Information

Product

MAP3K4 Antibody

Description

Affinity Purified Mitogen-activated protein kinase kinase kinase 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1558-1608 on human MAP3K4 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.50 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q9Y6R4

Overview

Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm > perinuclear region

Expression

Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.

Structure

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Alternative Nomenclature

dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) antibody

FLJ42439 antibody

KIAA0213 antibody

MAP three kinase 1 antibody

MAP/ERK kinase kinase 4 antibody

MAP3K4 antibody

MAPKKK4 antibody

MEK kinase 4 antibody

MEKK4 antibody

Mitogen-activated protein kinase kinase kinase 4 antibody

MTK1 antibody

predicted protein of HQ0412 antibody

PRO0412 antibody

RP3-473J16.4 antibody

SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of antibody

General Information

Product

PINK1 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase PINK1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to unique amino acid sequence on human PINK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9BXM7

Overview

During cellular stress it serves to protect against mitochondrial dysfunction through the phosphorylation of mitochondrial proteins. Uses selective autophagy to clear mitochondria that has been damaged.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Mitochondrion outer membrane; Single-pass membrane protein, Cytoplasm

Expression

Expression is detected at high levels in the heart, skeletal muscles and testis.  Expression is detected at lower levels in the brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine.

Structure

Interacts with PARK2 and FBXO7

Alternative Nomenclature

BRPK antibody

FLJ27236 antibody

mitochondrial antibody

PARK 6 antibody

Phosphatase and Tensin Homolog antibody

PINK 1 antibody

Protein kinase BRPK antibody

PTEN induced putative kinase 1 antibody

Serine/threonine-protein kinase PINK1 antibody

General Information

Product

cGKI Antibody

Description

Affinity Purified cGMP-dependent protein kinase 1 Antibody

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 609-659 on human protein kinase G alpha subunit.

Accession #

NCBI:  NP_001091982.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.64-0.72 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:500

Protein

Uniprot #

Q13976

Overview

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm

Expression

Primarily expressed in lung and placenta

Structure

Isoform alpha: parallel homodimer or heterodimer and also heterotetramer. Interacts directly with PPP1R12A. Non-covalent dimer of dimer of PRKG1-PRKG1 and PPP1R12A-PPP1R12A.

Alternative Nomenclature

cGK 1 antibody

cGKI alpha antibody

CGKI antibody

cGKI beta antibody

cGMP-dependent protein kinase 1 antibody

DKFZp686K042 antibody

FLJ36117 antibody

KGPB antibody

MGC71944 antibody

pkg antibody

PRKG1 antibody

PRKGR1A antibody

PRKGR1B antibody

General Information

Product

RIPK4 Antibody

Description

Affinity Purified Receptor-interacting Serine/Threonine-protein 

kinase 4 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 250-300 on human RIPK4 protein.

Accession #

NCBI:  NP_065690.2

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55-0.75 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9H4D1

Overview

ATP binding; Protein Serine/Threonine kinase activity

Structure

Contains 10 ANK repeats

Alternative Nomenclature

ANKK2 antibody

ANKRD 3 antibody

Ankyrin repeat domain 3 antibody

DIK antibody

PKC delta interacting protein kinase antibody

PKK antibody

protein kinase C-associated kinase antibody

Receptor interacting serine/threonine protein kinase 4 antibody

Serine/threonine protein kinase ANKRD3 antibody

General Information

Product

ROCK1 Antibody

Description

Affinity Purified Rho-associated protein kinase 1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Monkey, Mouse, Pig, Rat

Immunogen

Synthetic peptide taken within amino acid region spanning 850-900 on human ROCK1 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q13464

Overview

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Golgi apparatus membrane; Peripheral membrane protein, Cell projection

Expression

Detected in blood platelets.

Structure

Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner.

Alternative Nomenclature

coiled-coil-containing protein kinase 1 antibody

NY REN 35 antigen antibody

p160 Rhoassociated coiled coil-forming protein kinase antibody

p160 ROCK1 antibody

PRO0435 antibody

Renal carcinoma antigen NY REN 35 antibody

Rho associated coiled coil containing protein kinase 1 antibody

Rho-alpha kinase antibody

Rho-associated protein kinase 1 antibody

Rok antibody

rokalpha antibody

General Information

Product

ROCK2 Antibody

Description

Affinity Purified Rho-associated protein kinase 2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 1300-1388 on human ROCK2 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DOT Blot

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

O75116

Overview

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4.

Molecular Function

Kinase, Serine/threonine-protein kinase, Transferase

Subcellular Location

Cytoplasm, Cell membrane; Peripheral membrane protein, Nucleus

Structure

Homodimer. Interacts with IRS1, RHOB and RHOC. Interacts with RHOA (activated by GTP), PPP1R12A, CHORDC1, SORL1, EP300 and BRCA2. Interacts with NPM1 and this interaction enhances its activity. Interacts with RAF1.

Alternative Nomenclature

coiled-coil-containing protein kinase 2 antibody

KIAA0619 antibody

p164 ROCK 2 antibody

Rho associated, coiled coil containing protein kinase II antibody

ROK alpha antibody

General Information

Product

NAK Antibody

Description

Affinity Purified Serine/Threonine-protein kinase TBK1 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within second intracecllular loop of human TBK1 protein.

Accession #

NCBI:  NP_037386

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.52-0.57 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

Q9UHD2

Overview

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C. Phosphorylates Borna disease virus (BDV) P protein.

Molecular Function

ATP binding; Nucleic acid binding; Protein serine/threonine kinase activity

Subcellular Location

Cytoplasm

Expression

Ubiquitous with higher expression in testis. Expressed in the ganglion cells, nerve fiber layer and microvasculature of the retina.

Structure

Homodimer

Alternative Nomenclature

NAK antibody

NF kappa B activating kinase antibody

Serine/threonine protein kinase TBK 1 antibody

T2K antibody

TANK antibody

TANK binding kinase 1 antibody

TBK 1 antibody

TRAF2 antibody

TRIF antibody

General Information

Product

ULK1 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase ULK1 Antibody

Verified Applications

ELISA, IF, IP, WB

Host

Rabbit

Species Cross Reactivity

Bovine, Human, Monkey, Mouse, Panda

Immunogen

Synthetic peptide taken within amino acid region 1000-1050 on mouse ULK1 protein.

Accession #

NCBI:  NP_003556.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.58 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:10,000

Immunoprecipitation

1:200

Western Blot

1:250

Protein

Uniprot #

O75385

Overview

Involved in autophagy in response to starvation. Plays a role early in neuronal differentiation and is required for granule cell axon formation.

Molecular Function

ATP Binding, protein serine/threonine kinase activity, Rab GTPase binding, protein complex binding

Subcellular Location

Cytoplasm, cytosol, preautphagosomal structure. (Note: under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome)

Expression

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Alternative Nomenclature

ATG1 autophagy related 1 homolog antibody

ATG1A antibody

Autophagy related protein 1 homolog antibody

hATG1 antibody

Serine/threonine-protein kinase ULK1 antibody

ULK1 antibody

Unc 51 like kinase 1 antibody

Unc-51 like kinase 1 (C. elegans) antibody

UNC51 antibody

General Information

Product

ULK2 Antibody

Description

Affinity Purified Serine/Threonine-protein kinase ULK2 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 550-600 on rat ULK2 protein.

Accession #

NCBI:  NP_001178574.1

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.55 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

DB

1:10,000

ELISA

1:10,000

Western Blot

1:500

Protein

Uniprot #

D3ZUD3

Overview

Regulation of actin dynamics for phagocytic cup formation.

Alternative Nomenclature

ATG1B antibody

KIAA0623 antibody

Serine/threonine protein kinase ULK2 antibody

ULK2 antibody

Unc 51 like autophagy activating kinase 2 antibody

Unc 51 like kinase 2 antibody